| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 199,631 | G→T | 7.6% | A41A (GCC→GCA) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 199,631 | 0 | G | T | 7.6% | 247.6 / 4.8 | 79 | A41A (GCC→GCA) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (48/24); new base T (4/2); total (52/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01 | |||||||||||
CCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCA > minE/199564‑199695 | ccAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcg > 1:4296210/1‑70 (MQ=255) cAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcgc < 1:4101298/71‑3 (MQ=255) agttCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGcc < 1:1546961/68‑1 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac > 1:3109691/1‑70 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac > 1:4920648/1‑70 (MQ=255) cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat > 1:661592/1‑68 (MQ=255) cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat > 1:2335649/1‑68 (MQ=255) aaCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac < 1:553301/69‑1 (MQ=255) aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTACTGCAACCTGCTGTTCGGCACCAACAAt < 1:2688514/71‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc < 1:3783933/71‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc > 1:3192328/1‑71 (MQ=255) cAGGCCTTATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGc > 1:732471/1‑71 (MQ=255) aGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGcc < 1:946684/71‑1 (MQ=255) ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCa > 1:2362217/1‑66 (MQ=255) ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACg > 1:4402586/1‑66 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg > 1:2581423/1‑51 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg > 1:2293087/1‑51 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt > 1:2912895/1‑71 (MQ=255) cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt > 1:4797009/1‑71 (MQ=255) atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGAc > 1:1435920/1‑69 (MQ=255) atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCAGCACCAACAATCACGCCGACtt < 1:5226942/71‑1 (MQ=255) tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt < 1:4599779/71‑1 (MQ=255) atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt < 1:4870568/70‑1 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGc > 1:3245063/1‑48 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCa > 1:3503704/1‑49 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCACCAATc > 1:1993699/1‑58 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTaa > 1:4701123/1‑71 (MQ=255) tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTa < 1:4414412/70‑1 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:5197455/1‑71 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:3959560/1‑71 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:4436240/1‑71 (MQ=255) ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt > 1:2851138/1‑71 (MQ=255) tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg > 1:3717946/1‑71 (MQ=255) tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg > 1:3724639/1‑71 (MQ=255) tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGa > 1:3354070/1‑61 (MQ=255) tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtgtg < 1:2101692/71‑1 (MQ=255) cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg > 1:2120852/1‑71 (MQ=255) tttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATc > 1:291920/1‑52 (MQ=255) tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg > 1:5155188/1‑68 (MQ=255) tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGtt < 1:345309/70‑1 (MQ=255) cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTg > 1:121920/1‑71 (MQ=255) cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac < 1:1999374/43‑1 (MQ=255) tttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtt > 1:1769013/1‑71 (MQ=255) tCTGCGCGCCTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt > 1:5476276/1‑71 (MQ=255) tCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACg > 1:1042255/1‑44 (MQ=255) tCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt > 1:5141071/1‑71 (MQ=255) cTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttt > 1:5647715/1‑71 (MQ=255) cTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt < 1:3818655/70‑1 (MQ=255) gcgcGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTg < 1:1765505/60‑1 (MQ=255) cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttt < 1:4991990/68‑1 (MQ=255) cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa < 1:5305873/70‑1 (MQ=255) cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa < 1:4383731/70‑1 (MQ=255) cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa < 1:4940091/70‑1 (MQ=255) gcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCg > 1:3226176/1‑42 (MQ=255) cgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATcc > 1:2961040/1‑71 (MQ=255) cgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATcc > 1:476340/1‑71 (MQ=255) gACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCt < 1:2163639/71‑1 (MQ=255) gACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCt > 1:2461757/1‑71 (MQ=255) gACTTCCGCGACATCTTGTTCTGCGCCATTTATAACGCCAACTTTAATGTGTTTTGCATcgct > 1:2968153/1‑60 (MQ=255) tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGAcc > 1:2699028/1‑71 (MQ=255) tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGAc > 1:2299905/1‑70 (MQ=255) cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg > 1:2364959/1‑71 (MQ=255) cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg > 1:5150116/1‑71 (MQ=255) tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg > 1:3483311/1‑70 (MQ=255) tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg > 1:450319/1‑70 (MQ=255) tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg > 1:697501/1‑70 (MQ=255) gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt < 1:1587460/56‑1 (MQ=255) gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt < 1:62238/56‑1 (MQ=255) gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt < 1:2766342/56‑1 (MQ=255) agccTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTc < 1:3873994/55‑1 (MQ=255) aCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTg > 1:1013324/1‑62 (MQ=255) ccTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCAt > 1:4334295/1‑57 (MQ=255) ctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTAcca < 1:4673989/71‑1 (MQ=255) ttCGGCACCAACAATCACGCCGACTTTAATGTGGt < 1:1829858/35‑1 (MQ=255) ttCGGCACCAACAATCACGCCGACTTTAATGTGGt < 1:1744081/35‑1 (MQ=255) cGGCACCAACAATCACGCCGACTTTAATGTGGTTTgg > 1:1157424/1‑37 (MQ=255) cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGa > 1:1412544/1‑38 (MQ=255) cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGa > 1:3885790/1‑38 (MQ=255) cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt > 1:218466/1‑43 (MQ=255) cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt > 1:5657249/1‑43 (MQ=255) cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt > 1:2723486/1‑43 (MQ=255) | CCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCA > minE/199564‑199695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |