| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,731 | A→G | 23.0% | S446S (TCT→TCC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,731 | 0 | A | G | 23.0% | 156.1 / 44.5 | 87 | S446S (TCT→TCC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (40/27); new base G (12/8); total (52/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGA > minE/1014664‑1014800 | gctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa > 1:3249287/1‑71 (MQ=255)gctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGag > 1:472056/1‑70 (MQ=255) ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGa < 1:3328560/69‑1 (MQ=255) ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCAACATAAGa < 1:2458362/69‑1 (MQ=255) gactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc > 1:2740101/3‑70 (MQ=255) ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccat > 1:919753/1‑71 (MQ=255) ctgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccat < 1:635382/71‑1 (MQ=255) tgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccata > 1:33357/1‑70 (MQ=255) gCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:4892227/1‑71 (MQ=255) gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca < 1:3370554/65‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtcc > 1:2943055/1‑71 (MQ=255) taccTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct > 1:2604965/3‑70 (MQ=255) tGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg > 1:2437893/1‑71 (MQ=255) gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg > 1:153873/1‑70 (MQ=255) gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgt < 1:4345819/67‑1 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:3270859/2‑69 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:5384115/2‑69 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:3405713/2‑69 (MQ=255) accTTTCGCCAGCATGATCATGCCGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct > 1:5142767/2‑69 (MQ=255) cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcggcg > 1:2273606/1‑71 (MQ=255) tttCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtcca < 1:4624087/59‑1 (MQ=255) ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:3373890/1‑71 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt < 1:1482870/70‑1 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:4617686/1‑71 (MQ=255) gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCggtggt < 1:208187/71‑1 (MQ=255) gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGtg < 1:2335407/69‑2 (MQ=255) gCCAGCATGATCATACTTCCGCCCTGCGCTAGCAGTTGATCGACATAAGaa > 1:179943/1‑51 (MQ=255) ccAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGtt < 1:5185377/71‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatc < 1:3114174/52‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccg < 1:5585939/58‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:3198675/70‑1 (MQ=255) aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg < 1:3163240/71‑1 (MQ=255) aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg > 1:1936462/1‑71 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:5507567/66‑1 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2295277/1‑71 (MQ=255) tgttCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCa < 1:3217070/68‑1 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1444583/1‑66 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc < 1:4603298/70‑1 (MQ=255) gATCATACTTCCGCCCTGTGCTTGCAGTTGATCGACATAAGaa < 1:1828306/43‑1 (MQ=255) gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt < 1:2871881/64‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:2968097/1‑49 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:3933305/1‑49 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:4334084/43‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:430467/43‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:3905659/43‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:520481/1‑68 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:1201293/39‑1 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:5372804/39‑1 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:1640576/39‑1 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg > 1:4616898/1‑60 (MQ=255) aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAagag > 1:5288592/1‑70 (MQ=255) aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:276670/1‑35 (MQ=255) aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:917123/1‑35 (MQ=255) ctgccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAc > 1:1768185/4‑70 (MQ=255) tCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:3303521/1‑70 (MQ=255) ccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAcctg < 1:2496049/70‑3 (MQ=255) ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCAGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:532879/1‑69 (MQ=255) cGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca > 1:1688855/1‑36 (MQ=255) cGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca > 1:4922593/1‑36 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:3919429/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:73698/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:3284340/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:5226654/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:2758279/1‑58 (MQ=255) ttGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:1779207/42‑1 (MQ=255) ttGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAcctg < 1:2145841/59‑3 (MQ=255) tGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc > 1:1543561/1‑71 (MQ=255) tGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAgtga < 1:5144256/53‑1 (MQ=255) tGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAccgg > 1:3406880/1‑68 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg < 1:3510167/39‑1 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg < 1:2092348/39‑1 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1098524/1‑40 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1125083/1‑40 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:61690/1‑40 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTcc > 1:2276558/1‑71 (MQ=255) ttGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa < 1:2636206/44‑1 (MQ=255) ttGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa < 1:3044223/44‑1 (MQ=255) agATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAccg > 1:1166034/2‑62 (MQ=255) agATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAcc > 1:4609802/2‑62 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1441623/1‑36 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa > 1:4746208/1‑42 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:5664825/1‑44 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAag > 1:63370/1‑43 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGt < 1:2935683/70‑1 (MQ=255) tCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGttt > 1:116620/1‑70 (MQ=255) aCATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGc < 1:2207995/71‑1 (MQ=255) aTAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCg > 1:1045288/1‑71 (MQ=255) aaGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGa > 1:3023545/1‑71 (MQ=255) | GCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGA > minE/1014664‑1014800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |