| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,843 | C→T | 18.9% | G409E (GGG→GAG) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,843 | 0 | C | T | 18.9% | 230.0 / 41.5 | 107 | G409E (GGG→GAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (46/40); new base T (8/12); total (54/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
ACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCG > minE/1014779‑1014911 | aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa > 1:5414016/1‑70 (MQ=255)aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa > 1:3814459/1‑70 (MQ=255)aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAc > 1:101591/1‑71 (MQ=255) ccTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAcc > 1:4527079/1‑71 (MQ=255) tCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAcc > 1:2011655/1‑68 (MQ=255) tCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt > 1:3026398/1‑70 (MQ=255) cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGccc < 1:2412323/60‑1 (MQ=255) ttttGGCCGGACGCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatccatc > 1:4626157/1‑71 (MQ=255) ttttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:1013865/63‑1 (MQ=255) ttttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatccatc > 1:3414559/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcca > 1:4590974/1‑67 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3490139/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1345685/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2099960/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1742759/1‑71 (MQ=255) ggCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGc < 1:5612886/70‑1 (MQ=255) ccGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:4025183/71‑1 (MQ=255) ccGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:3958427/71‑1 (MQ=255) cGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt < 1:386899/71‑1 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:5068988/71‑1 (MQ=255) ggACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCttt < 1:4819662/71‑1 (MQ=255) cccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4683493/1‑71 (MQ=255) gcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:4713331/71‑1 (MQ=255) tagtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:5550914/63‑1 (MQ=255) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCggc > 1:1577843/1‑43 (MQ=12) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCggc > 1:1971180/1‑43 (MQ=12) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCggc > 1:2740048/1‑43 (MQ=12) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGt > 1:591435/1‑71 (MQ=255) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGt < 1:159008/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:2701980/58‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:669181/58‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4561844/1‑63 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:5614760/70‑1 (MQ=255) aaaTCGGATGATCTTTGATGTACTGCGGCAGCCCt > 1:3967715/1‑35 (MQ=255) aaaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCAc < 1:1106746/39‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt > 1:4673677/1‑41 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt > 1:2718549/1‑41 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2767455/1‑68 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:5363902/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:4459167/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:3063084/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg < 1:3039072/71‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg < 1:2528025/71‑1 (MQ=255) aTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg < 1:1891405/64‑1 (MQ=255) aTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg < 1:571955/64‑1 (MQ=255) aTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCg > 1:2889820/1‑39 (MQ=255) aTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCg > 1:2794645/1‑39 (MQ=255) tCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCa < 1:2257829/71‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:5657746/54‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTgg < 1:5341876/63‑1 (MQ=255) cGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa > 1:76916/1‑71 (MQ=255) gggtgatCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAat < 1:333256/68‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4045622/1‑56 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:3956383/1‑56 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:2865223/70‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:1704753/57‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:584630/71‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:401998/71‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:845235/57‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:793295/57‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATc < 1:3127483/71‑1 (MQ=255) ggatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:5177737/2‑69 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATc > 1:2108383/1‑70 (MQ=255) atCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:1608258/47‑1 (MQ=255) atCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAatat < 1:1186710/68‑1 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:1742229/1‑53 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:1096361/1‑53 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:5089026/65‑1 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:4376413/1‑71 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:3013531/1‑71 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1976161/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1768194/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1393894/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:3973381/1‑47 (MQ=255) ttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:1243314/1‑71 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:1014956/67‑1 (MQ=255) gATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccc < 1:3442358/70‑2 (MQ=255) aTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccca < 1:2628690/70‑3 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACcgacg > 1:2855539/1‑69 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:1044178/71‑1 (MQ=255) tACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt < 1:2416040/71‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:2640018/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:4970889/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:3486280/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:1117566/53‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:3857005/53‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcga > 1:1522583/1‑61 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATaa > 1:4373141/1‑71 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa < 1:2024512/70‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa > 1:4221716/1‑70 (MQ=255) gCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGAGTGAGCAat > 1:5339659/1‑52 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCg > 1:4918468/1‑71 (MQ=255) ggCAGCGCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3237745/36‑1 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3230830/36‑1 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCg < 1:3571505/70‑1 (MQ=255) cAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:5531401/1‑56 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:2042683/61‑1 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:359039/1‑71 (MQ=255) gCCCTTCACCGTAATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:5290922/61‑1 (MQ=255) cccTTCACCGTTATCCATCCGCTCTTTCAGTTTgg < 1:1365618/35‑1 (MQ=255) | ACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCG > minE/1014779‑1014911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |