| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,852 | T→G | 14.5% | N406T (AAC→ACC) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,852 | 0 | T | G | 14.5% | 242.3 / 40.7 | 117 | N406T (AAC→ACC) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (51/49); new base G (5/12); total (56/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
TCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAA > minE/1014783‑1014922 | tCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt > 1:3026398/1‑70 (MQ=255) ttttGGCCGGACGCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatccatc > 1:4626157/1‑71 (MQ=255) ttttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt < 1:1013865/63‑1 (MQ=255) ttttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatccatc > 1:3414559/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTatcca > 1:4590974/1‑67 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3490139/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1345685/1‑71 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2099960/1‑71 (MQ=255) ggCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGc < 1:5612886/70‑1 (MQ=255) ccGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:3958427/71‑1 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:5068988/71‑1 (MQ=255) ggACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCttt < 1:4819662/71‑1 (MQ=255) cccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg < 1:2653573/71‑1 (MQ=255) cccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4683493/1‑71 (MQ=255) gcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:4713331/71‑1 (MQ=255) tagtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:855344/63‑1 (MQ=255) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGt > 1:591435/1‑71 (MQ=255) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGt < 1:159008/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:2701980/58‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:669181/58‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4561844/1‑63 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:5614760/70‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt > 1:2009132/1‑41 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt > 1:2718549/1‑41 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2767455/1‑68 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:4459167/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg < 1:3039072/71‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:5363902/1‑71 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg < 1:2528025/71‑1 (MQ=255) aTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg < 1:1891405/64‑1 (MQ=255) aTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg < 1:571955/64‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:5657746/54‑1 (MQ=255) cGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa > 1:76916/1‑71 (MQ=255) gggtgatCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAat < 1:333256/68‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4045622/1‑56 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:3956383/1‑56 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:2865223/70‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:5541178/71‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:1704753/57‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:401998/71‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:845235/57‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATc < 1:3127483/71‑1 (MQ=255) ggatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:5177737/2‑69 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATc > 1:2108383/1‑70 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCa > 1:304630/1‑71 (MQ=255) atCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:3620500/47‑1 (MQ=255) atCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAatat < 1:1186710/68‑1 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:1742229/1‑53 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:1096361/1‑53 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:5089026/65‑1 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:4376413/1‑71 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1393894/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1768194/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1976161/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:3973381/1‑47 (MQ=255) ttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:1243314/1‑71 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:1014956/67‑1 (MQ=255) gATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccc < 1:3442358/70‑2 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACcgacg > 1:2855539/1‑69 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:1044178/71‑1 (MQ=255) tACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt < 1:2416040/71‑1 (MQ=255) tACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt < 1:4260998/71‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:2640018/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:4970889/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:3857005/53‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcga > 1:1522583/1‑61 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATaa > 1:4373141/1‑71 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa > 1:4221716/1‑70 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa < 1:2024512/70‑1 (MQ=255) gCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGAGTGAGCAat > 1:5339659/1‑52 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCg > 1:4918468/1‑71 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3230830/36‑1 (MQ=255) cAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:5531401/1‑56 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:2042683/61‑1 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:1479363/1‑71 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:359039/1‑71 (MQ=255) gCCCTTCACCGTAATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:5290922/61‑1 (MQ=255) cccTTCACCGTTATCCATCCGCTCTTTCAGTTTgg < 1:1365618/35‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:3192557/1‑66 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:1772105/1‑70 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:348849/71‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5256898/71‑1 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc < 1:16583/51‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5148124/69‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:4027284/1‑71 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCAACGATAATCGTGCCGTTAAg > 1:174298/1‑71 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCTTGAGCAATATCAcgaga > 1:3351006/1‑47 (MQ=39) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgaccga > 1:511593/1‑51 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2372162/1‑45 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg < 1:321464/70‑1 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:4912231/1‑71 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCg < 1:2110683/71‑1 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:781696/1‑38 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:883362/1‑38 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:4881699/1‑38 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:3509224/43‑1 (MQ=255) gTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGAt > 1:2827710/1‑70 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:4098501/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3658776/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATaa < 1:2487399/71‑1 (MQ=255) | TCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAA > minE/1014783‑1014922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |