| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,014,912 | T→G | 7.4% | N386T (AAC→ACC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,014,912 | 0 | T | G | 7.4% | 305.1 / 17.4 | 121 | N386T (AAC→ACC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (53/59); new base G (2/7); total (55/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
CTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAG > minE/1014844‑1014982 | cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:1772105/1‑70 (MQ=255)cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:5052641/1‑70 (MQ=255)cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5256898/71‑1 (MQ=255)cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:348849/71‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5148124/69‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:4027284/1‑71 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCAACGATAATCGTGCCGTTAAg > 1:174298/1‑71 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:4912231/1‑71 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCg < 1:2110683/71‑1 (MQ=255) gTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGAt > 1:2827710/1‑70 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATaa < 1:4427120/71‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:1676986/68‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:792600/68‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:2951692/68‑1 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAg > 1:1428937/1‑70 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:2569817/71‑1 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:3308024/71‑1 (MQ=255) gCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaa < 1:3616907/71‑1 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa > 1:3602321/1‑71 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa < 1:4951145/71‑1 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc < 1:4071831/69‑1 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc < 1:4596425/69‑1 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa > 1:3061272/1‑52 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa > 1:591413/1‑52 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa > 1:5499171/1‑52 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:2489260/64‑1 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:1602650/1‑71 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:1814396/1‑70 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:3911465/70‑1 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:4176942/70‑1 (MQ=255) aGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa > 1:244145/1‑64 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:1345450/71‑1 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:5365638/71‑1 (MQ=255) tGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:528083/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:5280783/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:2852516/1‑71 (MQ=255) gTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc > 1:5142449/1‑70 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:102225/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:1396740/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:501252/1‑71 (MQ=255) cAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTa > 1:3764832/1‑47 (MQ=255) cAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTa > 1:4638246/1‑47 (MQ=255) cAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTa > 1:5048612/1‑47 (MQ=255) atatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1835641/63‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATa < 1:4929802/36‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGc > 1:5199266/1‑70 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCg < 1:2578893/71‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg < 1:4250037/40‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg < 1:3734374/40‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc > 1:3514468/1‑47 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc > 1:743325/1‑47 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:3412687/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1075172/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4159673/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3647118/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:5663141/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3168626/1‑71 (MQ=255) cgaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt < 1:3468341/71‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:3293636/40‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:2712482/71‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:3212204/71‑1 (MQ=255) ccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:4707559/45‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGaa < 1:1061250/38‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc > 1:3748881/1‑68 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:2169117/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:703900/71‑1 (MQ=255) ccacgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATctct > 1:5406507/3‑71 (MQ=255) gacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCtt < 1:5125769/71‑1 (MQ=255) cgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTTCGAAAGCTGGGCGAGGATCTCTTTc < 1:1733960/71‑1 (MQ=255) cgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCttt > 1:4447970/1‑70 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1081887/49‑1 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:752029/49‑1 (MQ=255) aTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACggg < 1:3532737/38‑1 (MQ=255) aTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCAt < 1:2848773/71‑1 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1669128/1‑50 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4535664/1‑50 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:149437/1‑37 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:3903844/1‑37 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGAt > 1:2736351/1‑38 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:5418795/1‑43 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCg > 1:2919060/1‑71 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCg > 1:419350/1‑71 (MQ=255) aTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCg < 1:2195968/70‑1 (MQ=255) tggTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCttt < 1:4478725/62‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGa > 1:50036/1‑54 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:2716435/1‑55 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:2490004/1‑55 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:2305385/59‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGa > 1:4508695/1‑71 (MQ=255) tGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCg > 1:4963361/1‑70 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3337592/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3604627/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:876619/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGttt < 1:30886/71‑2 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:1923949/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:3792735/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:5584027/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:1318515/71‑1 (MQ=255) ttAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1960752/1‑50 (MQ=255) ttAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:932552/1‑71 (MQ=255) | CTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAG > minE/1014844‑1014982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |