| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,037 | T→C | 26.1% | K344K (AAA→AAG) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,037 | 0 | T | C | 26.1% | 98.7 / 37.8 | 65 | K344K (AAA→AAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (26/22); new base C (7/10); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGT > minE/1014968‑1015107 | cATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatatat < 1:5345646/70‑1 (MQ=255) gcGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTg < 1:1535543/70‑1 (MQ=255) ggTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTg < 1:3973303/69‑1 (MQ=255) ttAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTgggt > 1:3086546/1‑68 (MQ=255) ttAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc > 1:994112/1‑64 (MQ=255) ttAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc > 1:4762380/1‑64 (MQ=255) ttAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGAt > 1:1242021/1‑70 (MQ=255) tAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGttg > 1:515249/1‑70 (MQ=255) tAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGttg > 1:4670912/1‑70 (MQ=255) tAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTa > 1:4273965/1‑71 (MQ=255) aaGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGttgt > 1:836348/1‑69 (MQ=255) aaGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTAt > 1:3408586/1‑71 (MQ=255) aaCGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTc > 1:4136100/1‑69 (MQ=255) gcgcACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGtt > 1:1228562/1‑71 (MQ=255) ttcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt > 1:3514730/3‑71 (MQ=255) tcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt < 1:3939654/69‑1 (MQ=255) tcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt < 1:2587755/69‑1 (MQ=255) gcACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGtttt < 1:1686862/71‑1 (MQ=255) cACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGtttt < 1:2858984/70‑1 (MQ=255) ccGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAttt < 1:3619840/46‑1 (MQ=255) cGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa > 1:2906151/1‑71 (MQ=255) cGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa > 1:5163860/1‑71 (MQ=255) cGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa > 1:189977/1‑71 (MQ=255) cGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa > 1:788805/1‑71 (MQ=255) gCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGAt > 1:5046768/1‑71 (MQ=255) gCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGAt < 1:3122600/71‑1 (MQ=255) cTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATc < 1:4133638/71‑1 (MQ=255) cTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATc < 1:3000213/71‑1 (MQ=255) cTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATc < 1:4118388/71‑1 (MQ=255) cGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCa < 1:290012/70‑1 (MQ=255) cGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCa < 1:1328439/70‑1 (MQ=255) gCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:5063043/70‑1 (MQ=255) gCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:2193112/70‑1 (MQ=255) gCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:2727331/70‑1 (MQ=255) gCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:3180425/70‑1 (MQ=255) ccTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt < 1:2532187/71‑1 (MQ=255) ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGa > 1:5271891/1‑43 (MQ=255) ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGAt < 1:1672559/44‑1 (MQ=255) ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTa > 1:3055530/1‑46 (MQ=255) ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTAGATTATGTTCCAGTTTTTCGATc < 1:796523/64‑1 (MQ=255) tctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATccc > 1:376745/1‑71 (MQ=255) ctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATcccc > 1:2551854/1‑71 (MQ=255) ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt < 1:323718/69‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATg < 1:5297305/40‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATg < 1:3716480/40‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg > 1:1005824/1‑71 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:139382/71‑1 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3582069/71‑1 (MQ=255) cTTTGCGCAGCTCTTCAGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg > 1:5533592/1‑71 (MQ=255) tttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt > 1:2850554/1‑60 (MQ=255) tggcgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGtt > 1:3669571/3‑71 (MQ=255) tGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:1342141/1‑70 (MQ=255) gcgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCa < 1:2989331/54‑1 (MQ=255) cgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGa > 1:2236303/1‑70 (MQ=255) cgcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:4711421/70‑1 (MQ=255) tctgCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:1433269/66‑1 (MQ=255) gcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGAtt < 1:458306/71‑2 (MQ=255) cAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCt < 1:2214529/71‑1 (MQ=255) ctctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCt > 1:748674/1‑68 (MQ=255) tctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc > 1:2771865/1‑71 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt < 1:4661426/54‑1 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:3193210/1‑71 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:1180815/1‑71 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGAt > 1:3977174/1‑40 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGAt > 1:5459567/1‑40 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATACCCTGACGGTTGATCTTCGCt < 1:5277373/68‑1 (MQ=255) cGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa < 1:190477/71‑1 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg > 1:1319784/1‑39 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:1889572/1‑49 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:263020/1‑49 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata < 1:4241631/68‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1601331/71‑1 (MQ=255) tatTTGCGTGGATTATGTTCCAGTTTTTCGATCCAGATcccc > 1:182441/1‑42 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCa > 1:3672/1‑35 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCa > 1:2948184/1‑35 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:1151885/36‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:713891/36‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAg < 1:3442904/36‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:685870/1‑66 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:3493630/1‑66 (MQ=255) atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt > 1:691836/1‑42 (MQ=255) atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt > 1:2851571/1‑42 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt < 1:562367/71‑1 (MQ=255) | CATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGT > minE/1014968‑1015107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |