| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,079 | A→G | 35.2% | R330R (CGT→CGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,079 | 0 | A | G | 35.2% | 103.2 / 94.6 | 105 | R330R (CGT→CGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (40/28); new base G (14/23); total (54/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGC > minE/1015012‑1015147 | cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg > 1:1005824/1‑71 (MQ=255)cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3582069/71‑1 (MQ=255)cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:139382/71‑1 (MQ=255)cTTTGCGCAGCTCTTCAGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg > 1:5533592/1‑71 (MQ=255) tggcgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGtt > 1:3669571/3‑71 (MQ=255) tGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:1342141/1‑70 (MQ=255) cgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGa > 1:2236303/1‑70 (MQ=255) cgcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:4711421/70‑1 (MQ=255) tctgCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:1433269/66‑1 (MQ=255) gcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGAtt < 1:458306/71‑2 (MQ=255) cAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCt < 1:2214529/71‑1 (MQ=255) ctctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCt > 1:748674/1‑68 (MQ=255) tctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc > 1:2771865/1‑71 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:1180815/1‑71 (MQ=255) ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:3193210/1‑71 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATACCCTGACGGTTGATCTTCGCt < 1:5277373/68‑1 (MQ=255) cGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa < 1:190477/71‑1 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:263020/1‑49 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:1889572/1‑49 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata < 1:4241631/68‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1601331/71‑1 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:685870/1‑66 (MQ=255) tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:3493630/1‑66 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt < 1:562367/71‑1 (MQ=255) ttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt > 1:5620087/1‑70 (MQ=255) tggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGt > 1:3042698/3‑70 (MQ=255) tGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTc > 1:4645775/1‑53 (MQ=255) ggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTAGCTTTAATGTTACGGt > 1:5529572/2‑69 (MQ=255) gCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag < 1:493611/71‑1 (MQ=255) gCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:1544333/1‑71 (MQ=255) ccTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag < 1:2585167/70‑1 (MQ=255) cTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcagcg < 1:765686/71‑2 (MQ=255) tGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGtt < 1:2352472/42‑1 (MQ=16) ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2699696/1‑69 (MQ=255) ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:3118813/1‑69 (MQ=255) ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTA‑GGTcagcag > 1:2488653/1‑69 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:2269201/1‑54 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:4853656/1‑71 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:4837800/71‑1 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:4189154/1‑71 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:3866169/1‑71 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTAGCTTTGATATTACGGTCagcaga < 1:489632/70‑1 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:2264516/1‑53 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:5114168/1‑53 (MQ=255) ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:2001343/70‑1 (MQ=255) ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:943428/1‑69 (MQ=255) tgtGTTCCAGTTTTTCGATGCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagc < 1:4692495/67‑1 (MQ=255) gtGTTCCAGTTTTTCGATCCATATACCTTCGCGGTTGATTTTCGCTTTAATGTTACCGTCagcggagcag < 1:3984026/69‑1 (MQ=255) gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:812238/69‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:1212523/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:5354398/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:4598355/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3690239/71‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCtt < 1:2805948/46‑1 (MQ=25) tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCtt < 1:4131354/46‑1 (MQ=25) tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:19175/71‑1 (MQ=255) gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2179569/70‑1 (MQ=255) gTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:1146097/70‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:3603061/1‑71 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:1604107/1‑71 (MQ=255) ttCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga < 1:1525592/71‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATACCTTAGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga < 1:4348262/71‑1 (MQ=255) tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc > 1:3339198/1‑71 (MQ=255) ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:5284528/70‑1 (MQ=255) ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg > 1:55464/1‑71 (MQ=255) ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTa > 1:5324530/1‑50 (MQ=255) ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACg > 1:1805971/1‑71 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag < 1:425225/64‑1 (MQ=255) cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGGCAGCGGAGCAGGAGACGc < 1:4591599/71‑1 (MQ=255) aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagca > 1:2883009/1‑62 (MQ=255) aGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:5193442/41‑1 (MQ=19) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCtttaa > 1:3872506/1‑40 (MQ=16) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:988283/62‑1 (MQ=255) gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:1152191/62‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1178992/56‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1659209/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:939032/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:4554022/53‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3194806/63‑1 (MQ=255) ttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:610987/1‑41 (MQ=16) ttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:3770352/1‑41 (MQ=16) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:4828660/1‑39 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagca > 1:195876/1‑58 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga > 1:1768927/1‑63 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:5106137/1‑70 (MQ=255) cGATCCAGATACCTTCGGGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCTGGAGACGCCCATgcc < 1:959164/70‑1 (MQ=255) cGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgcc > 1:631225/1‑70 (MQ=255) gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccga < 1:2196846/71‑1 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1112260/1‑46 (MQ=255) ccAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg > 1:2889007/1‑71 (MQ=255) gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca < 1:4953286/43‑1 (MQ=255) ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg > 1:2236504/1‑70 (MQ=255) ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCACATACCGACCGGGCAg > 1:2294138/1‑70 (MQ=255) cccTCACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa < 1:3037613/71‑1 (MQ=255) cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGCCCGGGCAGGAt > 1:5521129/1‑70 (MQ=255) cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:3316979/1‑71 (MQ=255) cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:4567470/1‑71 (MQ=255) tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc > 1:2567914/1‑71 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgag > 1:5123485/1‑44 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc < 1:5279903/48‑1 (MQ=255) | CTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGC > minE/1015012‑1015147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |