| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,944 | G→A | 12.2% | intergenic (‑36/‑70) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,944 | 0 | G | A | 12.2% | 274.5 / 22.9 | 106 | intergenic (‑36/‑70) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (42/51); new base A (7/6); total (49/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
AAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAA > minE/217876‑218014 | aaGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCagag > 1:4435033/1‑69 (MQ=255)aaGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCagag > 1:3136085/1‑69 (MQ=255) aGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAg > 1:1915426/1‑71 (MQ=255) gAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGt > 1:1247148/1‑71 (MQ=255) aaaaTTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGtt < 1:636679/71‑1 (MQ=255) cAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc < 1:292028/71‑1 (MQ=255) aGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc > 1:818974/1‑70 (MQ=255) gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCTCAGAGAAGTTCGCcac < 1:4690056/66‑1 (MQ=255) gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:4370313/71‑1 (MQ=255) gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:264792/71‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATcc < 1:4332326/68‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATcc < 1:3681812/68‑1 (MQ=255) gCTCCGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:4354214/70‑1 (MQ=255) gAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAGGTTCGCCACAGCAATCCGAAACCa > 1:3018440/1‑70 (MQ=255) gAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc > 1:2903797/1‑71 (MQ=255) aaCTAATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa < 1:1460499/69‑1 (MQ=255) ataataAATTCTATGGTTCTGCTGATTTAACCGCAgaga > 1:5627028/1‑39 (MQ=255) ataataAATTCTATGGTTCTGCTGATTTAACCGCAGAGaa > 1:2025084/1‑40 (MQ=255) ataataAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:1599342/1‑48 (MQ=255) ataataAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:3649422/1‑48 (MQ=255) ataataAATTCTATAGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:1536852/1‑48 (MQ=255) taataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGt < 1:3306429/41‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg > 1:1605096/1‑71 (MQ=255) taaATTCTATGGTTCTGCTGATTTAACCGCAGAGaa > 1:4129252/1‑36 (MQ=255) taaATTCTATGGTTCTGCTGATTTAACCGCAGAGaa > 1:3261225/1‑36 (MQ=255) aaaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:1543275/1‑43 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTc > 1:5571926/1‑38 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTc > 1:4632689/1‑38 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACg < 1:4754874/66‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:3646884/52‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1485011/52‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:486304/52‑1 (MQ=255) tCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa > 1:1408437/1‑70 (MQ=255) tCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa > 1:5215661/1‑70 (MQ=255) tcagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGATAATAAg < 1:914249/68‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAg < 1:701778/68‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:1716862/4‑71 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2511962/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGa < 1:1747627/44‑1 (MQ=37) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGc > 1:1800812/3‑71 (MQ=255) agTTCTGCTGATTTAACCGCAGAGAAGATCGCCACAGCAATCCGAACCCACTGGCATGTCGAGAATAAGc > 1:2274820/2‑70 (MQ=255) gTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg < 1:2634944/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCt > 1:3081042/1‑70 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:1500413/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg > 1:5045809/1‑71 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2789026/49‑1 (MQ=255) tCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:2127757/1‑67 (MQ=255) ctgctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCAc > 1:4101966/1‑47 (MQ=25) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:116352/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:346636/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:1709404/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:3278884/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:2322516/70‑1 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:775220/1‑71 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:4592529/1‑71 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:3364865/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:2292927/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGCCATGTGGAGAATAAGCTg < 1:1386567/67‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1060539/39‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGaa < 1:888866/59‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg > 1:3438182/1‑47 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTga > 1:5483034/1‑46 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:2223114/68‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTAGCATGTGGAGAATAAGCTGCACTgg > 1:5356633/1‑71 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCg < 1:5199602/37‑1 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcactg < 1:3693486/70‑3 (MQ=255) tgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:2677556/37‑1 (MQ=255) gATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGcactg < 1:2472351/68‑3 (MQ=255) gATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCg > 1:134022/1‑71 (MQ=255) aTTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2922580/1‑59 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc > 1:1075564/1‑41 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:2494219/44‑1 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt > 1:4721210/1‑71 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:4158700/69‑1 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGt > 1:902225/1‑71 (MQ=255) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:456631/1‑71 (MQ=255) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:3839044/1‑62 (MQ=255) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt > 1:3662270/1‑69 (MQ=255) tAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt < 1:1294206/70‑1 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg > 1:2033247/1‑71 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:4052539/1‑57 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:3520978/1‑57 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:1009297/1‑71 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1689379/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3691445/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:2028959/70‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:4357549/71‑1 (MQ=255) cGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGgag < 1:2625925/46‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:4525289/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:1187589/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:320840/71‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2269167/3‑50 (MQ=37) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1464334/3‑71 (MQ=255) cAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcactg < 1:5480294/58‑3 (MQ=255) cAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:316088/1‑55 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:4280936/52‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg < 1:5525763/71‑1 (MQ=255) gagaAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2267324/71‑1 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:5014862/70‑1 (MQ=255) gaAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:2568315/1‑71 (MQ=255) | AAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAA > minE/217876‑218014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |