| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,184 | A→C | 38.9% | A295A (GCT→GCG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,184 | 0 | A | C | 38.9% | 46.9 / 148.3 | 121 | A295A (GCT→GCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (31/43); new base C (20/27); total (51/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
CAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCC > minE/1015116‑1015253 | cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGc < 1:44878/71‑1 (MQ=255) agGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGcgcg < 1:1667475/71‑1 (MQ=255) ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGcgcg > 1:5622372/1‑70 (MQ=255) ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGa < 1:4194267/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:550342/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:2178286/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:1877428/71‑1 (MQ=255) cGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAt > 1:515647/1‑71 (MQ=255) gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:2853639/71‑1 (MQ=255) cccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTgcgc < 1:2705980/62‑1 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac > 1:1605888/1‑71 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac > 1:3480242/1‑71 (MQ=255) atgccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc < 1:5183224/68‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:5133979/67‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:2331947/67‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:761644/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:4369366/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3721887/1‑71 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt > 1:3575939/1‑64 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:5179/67‑1 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTaccgc > 1:4794508/1‑68 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTaccgc > 1:3481542/1‑68 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:766822/71‑1 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:3918109/71‑1 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:4751028/71‑1 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3642065/1‑67 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca > 1:1116641/1‑70 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca > 1:4505366/1‑70 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:3716781/70‑1 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc > 1:5363327/1‑61 (MQ=255) cAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:868233/56‑1 (MQ=255) cAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTgcg > 1:1080147/1‑70 (MQ=255) aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:5593544/63‑1 (MQ=255) gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3962549/1‑71 (MQ=255) gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:4296266/71‑1 (MQ=255) gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:5543269/71‑1 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:1404639/1‑52 (MQ=255) tGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTa > 1:3893736/1‑52 (MQ=255) tGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGcc > 1:3596755/1‑69 (MQ=255) cgcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCaaa > 1:4584156/1‑58 (MQ=255) cgcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAg < 1:3368764/70‑1 (MQ=255) ccGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTa < 1:3373173/44‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2376671/71‑1 (MQ=255) gTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAc > 1:4623788/1‑68 (MQ=255) gTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGt < 1:3361534/41‑1 (MQ=19) tGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg < 1:2570077/56‑1 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAc < 1:502631/67‑1 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:4065708/1‑71 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGaa > 1:2317324/1‑36 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:1413253/1‑52 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt > 1:2604271/1‑71 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:5281815/71‑3 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:4418975/71‑3 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgt < 1:2940748/70‑2 (MQ=255) cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:4795824/49‑1 (MQ=255) cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:2038483/49‑1 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:2163767/71‑1 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:1041444/71‑1 (MQ=255) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTac > 1:5358333/1‑42 (MQ=19) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTa > 1:5089089/1‑41 (MQ=19) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:3620845/47‑1 (MQ=255) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:2529111/47‑1 (MQ=255) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:2535727/47‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGt < 1:3063156/35‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:404026/38‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAt < 1:653327/37‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:3543896/71‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2860717/71‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:2215510/40‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:2625634/40‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:1577095/40‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2447383/63‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2112087/63‑1 (MQ=255) cGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2560277/64‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:79475/1‑41 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:1013409/71‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:2654855/71‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAg > 1:5381085/1‑51 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1184460/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:4131335/70‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2829265/71‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2457002/71‑1 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAc > 1:2862767/1‑43 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:187400/1‑71 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:3904013/1‑71 (MQ=255) cgcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3186520/71‑1 (MQ=255) cgcgAATTTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttca > 1:4392976/1‑55 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:5358661/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:1347161/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:828371/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTggg > 1:3676836/1‑58 (MQ=255) gcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc > 1:2286505/1‑70 (MQ=255) aTGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTc < 1:2320369/71‑1 (MQ=255) aTGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt < 1:1610945/49‑1 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2910283/71‑1 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct > 1:199845/1‑47 (MQ=19) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:5068033/1‑68 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:2990150/1‑68 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3395182/59‑1 (MQ=255) aGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc < 1:89427/70‑1 (MQ=255) | CAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCC > minE/1015116‑1015253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |