| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,947 | G→A | 13.7% | intergenic (‑39/‑67) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,947 | 0 | G | A | 13.7% | 329.9 / 34.0 | 131 | intergenic (‑39/‑67) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (50/63); new base A (7/11); total (57/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
AGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATG > minE/217877‑218016 | aGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAg > 1:1915426/1‑71 (MQ=255) aaaaTTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGtt < 1:636679/71‑1 (MQ=255) aGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc > 1:818974/1‑70 (MQ=255) gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCTCAGAGAAGTTCGCcac < 1:4690056/66‑1 (MQ=255) gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:4370313/71‑1 (MQ=255) gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:264792/71‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATcc < 1:4332326/68‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATcc < 1:3681812/68‑1 (MQ=255) gCTCCGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:4354214/70‑1 (MQ=255) gAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc > 1:2903797/1‑71 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2669981/70‑1 (MQ=255) aaCTAATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa < 1:1460499/69‑1 (MQ=255) ataataAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:3649422/1‑48 (MQ=255) ataataAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:1599342/1‑48 (MQ=255) ataataAATTCTATAGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca > 1:1536852/1‑48 (MQ=255) taataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGt < 1:2218769/41‑1 (MQ=255) taataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGt < 1:3306429/41‑1 (MQ=255) taataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGc < 1:2927701/45‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg < 1:1512108/71‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg > 1:1605096/1‑71 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTc > 1:4632689/1‑38 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTc > 1:5571926/1‑38 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACg < 1:4754874/66‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:486304/52‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:3646884/52‑1 (MQ=255) tCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa > 1:5215661/1‑70 (MQ=255) tcagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGATAATAAg < 1:914249/68‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAg < 1:701778/68‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:1716862/4‑71 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2511962/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGa < 1:1747627/44‑1 (MQ=37) cagTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTg > 1:2283110/3‑54 (MQ=255) agTTCTGCTGATTTAACCGCAGAGAAGATCGCCACAGCAATCCGAACCCACTGGCATGTCGAGAATAAGc > 1:2274820/2‑70 (MQ=255) gTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg < 1:2634944/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCt > 1:3081042/1‑70 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:1500413/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATaa < 1:4777835/67‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg > 1:5045809/1‑71 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2789026/49‑1 (MQ=255) tCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:2127757/1‑67 (MQ=255) ctgctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCAc > 1:4101966/1‑47 (MQ=25) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:116352/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:1709404/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:2322516/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:3278884/70‑1 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:4592529/1‑71 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:775220/1‑71 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:2292927/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:3371385/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGCCATGTGGAGAATAAGCTg < 1:1386567/67‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1060539/39‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGaa < 1:888866/59‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa > 1:5249360/1‑43 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg > 1:3438182/1‑47 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTga > 1:5483034/1‑46 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:2223114/68‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTAGCATGTGGAGAATAAGCTGCACTgg > 1:5356633/1‑71 (MQ=255) gATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGcactg < 1:2472351/68‑3 (MQ=255) gATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCa < 1:4359350/47‑1 (MQ=255) gATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCg > 1:134022/1‑71 (MQ=255) aTTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2922580/1‑59 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGGAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:2536454/69‑1 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:4158700/69‑1 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGt > 1:902225/1‑71 (MQ=255) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACg > 1:1863310/1‑46 (MQ=25) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:3839044/1‑62 (MQ=255) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt > 1:3662270/1‑69 (MQ=255) tAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt < 1:1294206/70‑1 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:3520978/1‑57 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:1009297/1‑71 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1689379/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:2028959/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3691445/70‑1 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1220340/69‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:789490/59‑1 (MQ=255) cGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGgag < 1:3727569/46‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:1187589/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:320840/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:4525289/71‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1464334/3‑71 (MQ=255) cAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcactg < 1:5480294/58‑3 (MQ=255) cAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:316088/1‑55 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg < 1:5525763/71‑1 (MQ=255) gagaAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2267324/71‑1 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1425037/68‑1 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:4600385/70‑1 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:5014862/70‑1 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATaa < 1:3403752/44‑1 (MQ=255) gaAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:2568315/1‑71 (MQ=255) gaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa < 1:4244904/71‑1 (MQ=255) aaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:4014494/2‑46 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1060063/60‑1 (MQ=38) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:2818678/64‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:2452531/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:3358243/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:296888/1‑70 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:3090713/1‑46 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:1007590/1‑46 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:4839950/1‑70 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4325223/70‑1 (MQ=255) | AGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATG > minE/217877‑218016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |