| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,232 | T→C | 7.3% | E279E (GAA→GAG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,232 | 0 | T | C | 7.3% | 274.0 / 8.7 | 96 | E279E (GAA→GAG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (51/38); new base C (5/2); total (56/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGG > minE/1015165‑1015300 | gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:1013409/71‑1 (MQ=255)gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:2654855/71‑1 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1184460/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:4131335/70‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2829265/71‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2457002/71‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:3377907/71‑1 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:187400/1‑71 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:3904013/1‑71 (MQ=255) cgcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3186520/71‑1 (MQ=255) gcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc > 1:2286505/1‑70 (MQ=255) ggATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAAt > 1:2359578/1‑71 (MQ=255) aTGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTc < 1:2320369/71‑1 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2910283/71‑1 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:5068033/1‑68 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:2990150/1‑68 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:287885/59‑1 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3395182/59‑1 (MQ=255) gCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg < 1:5613530/71‑1 (MQ=255) cGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgc > 1:2203378/1‑70 (MQ=255) aGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgctg < 1:4441711/70‑4 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt > 1:4026848/1‑62 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt > 1:2533073/1‑62 (MQ=255) gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:5691118/1‑71 (MQ=255) tATTTATCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:1991901/51‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3916370/51‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:5644418/51‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:5335744/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:170607/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:2062810/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:4015888/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac > 1:2587251/1‑70 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3136481/45‑1 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:548298/45‑1 (MQ=255) tACCACCAAACTGCGCCCCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4337732/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:791727/43‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:2772074/43‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt > 1:2086504/1‑55 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg > 1:4687202/1‑61 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:5206192/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4518929/1‑71 (MQ=255) accaccAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCa > 1:3226614/1‑47 (MQ=255) accaccAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCa > 1:1342216/1‑47 (MQ=255) caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3732206/40‑1 (MQ=255) caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCgg < 1:4782366/71‑1 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:4364390/1‑71 (MQ=255) caAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAAc > 1:2584735/1‑71 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg > 1:3177950/1‑71 (MQ=255) aaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGc > 1:5115266/1‑71 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt < 1:3385621/48‑1 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGtt > 1:2185789/1‑55 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGc > 1:3749115/1‑71 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc < 1:2063972/49‑1 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:1751104/57‑1 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGcc > 1:310655/1‑71 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc > 1:4284153/1‑48 (MQ=255) cgcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:1220813/69‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:1358784/1‑61 (MQ=255) cccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:2871839/49‑1 (MQ=255) cccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:4259719/49‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:4973735/48‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:5649973/1‑71 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:4095523/1‑71 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt < 1:5475389/71‑1 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:4954005/1‑71 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1661203/1‑46 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1888053/1‑46 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1319329/1‑46 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:3869904/1‑70 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:5513014/1‑70 (MQ=255) gACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa < 1:2054361/71‑1 (MQ=255) ccAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt > 1:1683929/1‑46 (MQ=255) ccAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAtt > 1:7295/1‑71 (MQ=255) ccAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAtt < 1:3362463/71‑1 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc > 1:4994845/1‑35 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc > 1:3391593/1‑35 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc > 1:389368/1‑35 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTc > 1:4611896/1‑71 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTc > 1:1532803/1‑71 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCAAGTTCCACATcg > 1:4392533/1‑47 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgcg > 1:4825571/1‑46 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgc > 1:2410770/1‑45 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCt > 1:4712877/1‑71 (MQ=255) ttttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTc < 1:1593021/71‑1 (MQ=255) tttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc > 1:3523973/1‑71 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgcg > 1:4311236/1‑42 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc > 1:5560640/1‑53 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc > 1:3626270/1‑53 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc > 1:2753235/1‑53 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:4880941/70‑1 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc > 1:2950151/1‑70 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg > 1:1366151/1‑71 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa < 1:538864/57‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCTTGCTCAt < 1:3681184/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt < 1:1386262/53‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAt < 1:4761850/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAt < 1:3321426/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAt < 1:1211112/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:5049742/1‑69 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:5237853/1‑69 (MQ=255) | GAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGG > minE/1015165‑1015300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |