| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,959 | A→G | 20.6% | intergenic (‑51/‑55) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,959 | 0 | A | G | 20.6% | 313.6 / 75.7 | 160 | intergenic (‑51/‑55) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (49/78); new base G (15/18); total (64/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 163 total aligned reads displayed.
GATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGA > minE/217890‑218027 | gATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:4370313/71‑1 (MQ=255) gCTCCGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:4354214/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2669981/70‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg > 1:1605096/1‑71 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg < 1:1512108/71‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:486304/52‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1485011/52‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGATAATAAg < 1:914249/68‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAg < 1:701778/68‑1 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2511962/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGa < 1:1747627/44‑1 (MQ=37) cagTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTg > 1:2283110/3‑54 (MQ=255) gTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg < 1:2634944/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCt > 1:3081042/1‑70 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATaa < 1:4777835/67‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg > 1:5045809/1‑71 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2789026/49‑1 (MQ=255) ctgctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCAc > 1:4101966/1‑47 (MQ=25) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:346636/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:1709404/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:2322516/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:3278884/70‑1 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:4592529/1‑71 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:775220/1‑71 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:3371385/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:2292927/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGCCATGTGGAGAATAAGCTg < 1:1386567/67‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1060539/39‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGaa < 1:888866/59‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa > 1:5249360/1‑43 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg > 1:3438182/1‑47 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTga > 1:5483034/1‑46 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:2223114/68‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTAGCATGTGGAGAATAAGCTGCACTgg > 1:5356633/1‑71 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCg < 1:5199602/37‑1 (MQ=255) aTTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2922580/1‑59 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc > 1:1075564/1‑41 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:2494219/44‑1 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt > 1:4721210/1‑71 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:4158700/69‑1 (MQ=255) tttAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTc > 1:3650968/1‑71 (MQ=255) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACg > 1:1863310/1‑46 (MQ=25) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:456631/1‑71 (MQ=255) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt > 1:3662270/1‑69 (MQ=255) tAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt < 1:1294206/70‑1 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:4052539/1‑57 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:1009297/1‑71 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:2028959/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1689379/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3691445/70‑1 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1220340/69‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:789490/59‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:4357549/71‑1 (MQ=255) cGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGgag < 1:2625925/46‑1 (MQ=255) cGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGgag < 1:3727569/46‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2269167/3‑50 (MQ=37) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1464334/3‑71 (MQ=255) cAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcactg < 1:5480294/58‑3 (MQ=255) cAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:316088/1‑55 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg < 1:318990/41‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg < 1:1701365/41‑1 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1425037/68‑1 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:5014862/70‑1 (MQ=255) gaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:5564911/1‑71 (MQ=255) gaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa < 1:4244904/71‑1 (MQ=255) aaattCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:2987081/61‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4857679/71‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:3358243/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:2452531/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:1007590/1‑46 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:3090713/1‑46 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4325223/70‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:844892/71‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:231813/71‑1 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg > 1:3880512/2‑70 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:3931760/1‑47 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:5407114/1‑47 (MQ=255) cGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:4831018/1‑70 (MQ=255) cGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:3005613/1‑70 (MQ=255) gCCACAGCAATCCGAAACCTCTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga < 1:4296141/71‑1 (MQ=255) ccACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:3373769/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:2614983/71‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:479102/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:949904/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:2137598/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg > 1:258264/1‑56 (MQ=38) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:2563555/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3630250/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3734292/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:211418/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg < 1:1440846/52‑1 (MQ=255) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:1180948/1‑71 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2921521/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1755055/71‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTc < 1:3297961/47‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacg < 1:2897789/71‑1 (MQ=255) gCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:2762749/1‑71 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:93979/61‑1 (MQ=38) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:1723496/65‑1 (MQ=39) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:153989/65‑1 (MQ=39) | GATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGA > minE/217890‑218027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |