| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,256 | T→C | 21.5% | E271E (GAA→GAG) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,256 | 0 | T | C | 21.5% | 182.7 / 44.5 | 93 | E271E (GAA→GAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (47/26); new base C (10/10); total (57/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01 | |||||||||||
GTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAG > minE/1015190‑1015326 | gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:5691118/1‑71 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:5335744/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:2062810/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:170607/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:4015888/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac > 1:2587251/1‑70 (MQ=255) tACCACCAAACTGCGCCCCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4337732/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:5206192/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4518929/1‑71 (MQ=255) caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCgg < 1:4782366/71‑1 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:4364390/1‑71 (MQ=255) caAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAAc > 1:2584735/1‑71 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg > 1:3177950/1‑71 (MQ=255) aaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGc > 1:5115266/1‑71 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGtt > 1:2185789/1‑55 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGc > 1:3749115/1‑71 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:1751104/57‑1 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGcc > 1:310655/1‑71 (MQ=255) cgcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:1220813/69‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:1358784/1‑61 (MQ=255) cccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:2871839/49‑1 (MQ=255) cccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:4259719/49‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:4973735/48‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:4095523/1‑71 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:5649973/1‑71 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt < 1:5475389/71‑1 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:4954005/1‑71 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1888053/1‑46 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1661203/1‑46 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1319329/1‑46 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:5513014/1‑70 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:3869904/1‑70 (MQ=255) gACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa < 1:2054361/71‑1 (MQ=255) ccAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt > 1:1683929/1‑46 (MQ=255) ccAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAtt < 1:3362463/71‑1 (MQ=255) ccAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAtt > 1:7295/1‑71 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTc > 1:1532803/1‑71 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTc > 1:4611896/1‑71 (MQ=255) cAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCAAGTTCCACATcg > 1:4392533/1‑47 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgcg > 1:4825571/1‑46 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgc > 1:2410770/1‑45 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCt > 1:4712877/1‑71 (MQ=255) ttttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTc < 1:1593021/71‑1 (MQ=255) tttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc > 1:3523973/1‑71 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcgcg > 1:4311236/1‑42 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc > 1:5560640/1‑53 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc > 1:3626270/1‑53 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc > 1:2753235/1‑53 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:4880941/70‑1 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc > 1:2950151/1‑70 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg > 1:1366151/1‑71 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa < 1:538864/57‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCTTGCTCAt < 1:3681184/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt < 1:1386262/53‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAt < 1:3321426/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAt < 1:4761850/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAt < 1:1211112/56‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:5049742/1‑69 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:5237853/1‑69 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1156936/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:5597289/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:841212/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:825667/1‑71 (MQ=255) ttccagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAg < 1:2850847/66‑1 (MQ=255) aTCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGa > 1:3672237/1‑42 (MQ=255) aTCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGa > 1:3801326/1‑42 (MQ=255) ccagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTc > 1:659490/2‑49 (MQ=255) cagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtca < 1:3529388/70‑1 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGTCAGTtcat < 1:462331/70‑1 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat > 1:1069328/1‑70 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat > 1:2260021/1‑70 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat < 1:3075955/70‑1 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCCTTGGCAGTtcat < 1:3081069/70‑1 (MQ=255) gCAGTTCCTGTTCCAGCTGGATATCGCGGAACGCCTGACCATGTTCGTTCCCtt > 1:4227379/1‑54 (MQ=12) gCAGTTCCTGTTCCAGCTGGATATCGCGGAACGCCTGACCATGTTCGTTCCCtt > 1:1138379/1‑54 (MQ=12) gCAGTTCCTGTTCCAGCTGGATATCGCGGAACGCCTGACCATGTTCGTTCCCtt > 1:890307/1‑54 (MQ=12) gCAGTTCCTGTTCCAGCTGGATATCGCGGAACGCCTGACCATGTTCGTTCCCtt > 1:5273397/1‑54 (MQ=12) gCAGTTCCTGTTCCAGCTGGATATCGCGGAACGCCTGACCATGTTCGTTCCCtt > 1:4980568/1‑54 (MQ=12) gCAGTTCCTGTTCCAGCTGGATATCGCGGAACGCCTGACCATGTTCGTTCCCtt > 1:31893/1‑54 (MQ=12) gCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcatcg < 1:752292/71‑2 (MQ=255) aaTTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca > 1:2610039/1‑69 (MQ=255) ttCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCtt < 1:5444176/50‑1 (MQ=255) ttCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCtt < 1:3602112/50‑1 (MQ=255) cTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAt > 1:750885/1‑71 (MQ=255) ttttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAtt > 1:3862745/1‑70 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTTATCGTAATagt < 1:17031/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTaa > 1:3190261/1‑63 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:5569910/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:4404743/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:2284907/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4736138/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4515649/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4292728/68‑3 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgc > 1:983451/1‑71 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:4043971/40‑1 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:34462/40‑1 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:3129600/40‑1 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:1152013/40‑1 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAg > 1:4799669/1‑71 (MQ=255) | GTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAG > minE/1015190‑1015326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |