| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,298 | T→C | 23.8% | P257P (CCA→CCG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,298 | 0 | T | C | 23.8% | 152.3 / 48.1 | 88 | P257P (CCA→CCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (36/31); new base C (8/13); total (44/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCAC > minE/1015232‑1015368 | ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:5049742/1‑69 (MQ=255)ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:5237853/1‑69 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1156936/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:841212/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:825667/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:5597289/1‑71 (MQ=255) ttccagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAg < 1:2850847/66‑1 (MQ=255) cagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtca < 1:3529388/70‑1 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGTCAGTtcat < 1:462331/70‑1 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat > 1:2260021/1‑70 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat > 1:1069328/1‑70 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat < 1:3075955/70‑1 (MQ=255) aGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCCTTGGCAGTtcat < 1:3081069/70‑1 (MQ=255) gCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcatcg < 1:752292/71‑2 (MQ=255) aaTTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca > 1:2610039/1‑69 (MQ=255) cTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAt > 1:750885/1‑71 (MQ=255) ttttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAtt > 1:3862745/1‑70 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTTATCGTAATagt < 1:17031/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTaa > 1:3190261/1‑63 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:2284907/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:5569910/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:4404743/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4515649/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4736138/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4292728/68‑3 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgc > 1:983451/1‑71 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAg > 1:4799669/1‑71 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAg > 1:3122701/1‑46 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca > 1:5571181/1‑53 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc > 1:404647/1‑70 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc > 1:3446307/1‑70 (MQ=255) aTCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAg > 1:4489761/1‑52 (MQ=255) aTCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt > 1:4543378/1‑71 (MQ=255) aTCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtc < 1:3609211/45‑1 (MQ=255) aTCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtc < 1:5339815/45‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat < 1:4574071/46‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:694299/71‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:3077542/71‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:1834510/71‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt > 1:4235186/1‑71 (MQ=255) cgcgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGtt > 1:524866/1‑42 (MQ=255) cgcgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGtt > 1:751471/1‑42 (MQ=255) cgcgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGtt > 1:4184905/1‑42 (MQ=255) cgcgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGtt > 1:476484/1‑42 (MQ=255) cgcgGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGt > 1:4101945/1‑41 (MQ=25) cgcgGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcatc > 1:2080178/1‑46 (MQ=255) cgcgGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcat > 1:5415063/1‑45 (MQ=255) gcgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGaa > 1:2008294/1‑61 (MQ=255) gcgGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTa > 1:5257797/1‑48 (MQ=255) gcgGAACGCCTGACCATGTTCGTTACCTTCCGTCGGc > 1:5326464/1‑37 (MQ=16) gcgGAACGCCCGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTa > 1:3393111/1‑48 (MQ=19) cgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCAc < 1:2302416/71‑1 (MQ=255) cgGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcat < 1:5137597/43‑1 (MQ=25) ggAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAg < 1:2423036/47‑1 (MQ=255) aaCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTa > 1:1589301/1‑47 (MQ=255) aaCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTa > 1:2587117/1‑47 (MQ=255) aaCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTa > 1:5109643/1‑47 (MQ=255) gCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:5389410/71‑1 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:4389633/70‑1 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt > 1:1930855/1‑70 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt > 1:2418531/1‑70 (MQ=255) aCCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGt < 1:3751543/71‑1 (MQ=255) cGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTc < 1:1029168/71‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:1056117/62‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:2469337/62‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:4152522/62‑1 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAt > 1:1864333/1‑35 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtt < 1:4769520/59‑1 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGtt > 1:2281909/1‑71 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGtt > 1:5600145/1‑71 (MQ=255) cATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGcc < 1:3693853/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGTAAGCCa < 1:739269/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCa < 1:1418580/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCa < 1:62990/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTc < 1:4961902/69‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTc < 1:898968/69‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCt > 1:147904/1‑70 (MQ=255) ttCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCa < 1:3998619/43‑1 (MQ=255) ttCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCt > 1:2425928/1‑69 (MQ=255) tCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTccg > 1:2212573/1‑67 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:3626555/70‑1 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:5404010/70‑1 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:1177108/70‑1 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:5056388/70‑1 (MQ=255) cTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:4485363/69‑1 (MQ=255) ttCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTc < 1:2943563/43‑1 (MQ=255) cGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTc > 1:3795441/1‑71 (MQ=255) cGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTc < 1:1543930/71‑1 (MQ=255) ttGTCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:3827362/46‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt < 1:4443503/36‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt < 1:3484738/36‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt < 1:963552/36‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:4578205/46‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTcca > 1:2912347/1‑71 (MQ=255) tGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTccac > 1:5304233/1‑71 (MQ=255) | TTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCAC > minE/1015232‑1015368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |