| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,968 | C→T | 21.7% | intergenic (‑60/‑46) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,968 | 0 | C | T | 21.7% | 369.8 / 92.1 | 189 | intergenic (‑60/‑46) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (64/84); new base T (19/22); total (83/106) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 190 total aligned reads displayed.
GAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATA > minE/217901‑218037 | gAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAGGTTCGCCACAGCAATCCGAAACCa > 1:3018440/1‑70 (MQ=255) aaCTAATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa < 1:1460499/69‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg > 1:1605096/1‑71 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTg < 1:1512108/71‑1 (MQ=255) aaTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACg < 1:4754874/66‑1 (MQ=255) tCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa > 1:5215661/1‑70 (MQ=255) tcagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGATAATAAg < 1:914249/68‑1 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2511962/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGc > 1:1800812/3‑71 (MQ=255) agTTCTGCTGATTTAACCGCAGAGAAGATCGCCACAGCAATCCGAACCCACTGGCATGTCGAGAATAAGc > 1:2274820/2‑70 (MQ=255) gTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg < 1:2634944/71‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2789026/49‑1 (MQ=255) tCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:2127757/1‑67 (MQ=255) ctgctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCAc > 1:4101966/1‑47 (MQ=25) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:3278884/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:1709404/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:2322516/70‑1 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:775220/1‑71 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:3371385/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:2292927/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGCCATGTGGAGAATAAGCTg < 1:1386567/67‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGaa < 1:888866/59‑1 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg > 1:3438182/1‑47 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTga > 1:5483034/1‑46 (MQ=255) ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTAGCATGTGGAGAATAAGCTGCACTgg > 1:5356633/1‑71 (MQ=255) aTTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2922580/1‑59 (MQ=255) tttAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTc > 1:3650968/1‑71 (MQ=255) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACg > 1:1863310/1‑46 (MQ=25) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:456631/1‑71 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg > 1:2033247/1‑71 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:4052539/1‑57 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3691445/70‑1 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1220340/69‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:789490/59‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:4357549/71‑1 (MQ=255) cGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGgag < 1:3727569/46‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:320840/71‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1464334/3‑71 (MQ=255) cAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:316088/1‑55 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:4280936/52‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg < 1:5525763/71‑1 (MQ=255) gagaAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:2267324/71‑1 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1425037/68‑1 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:4600385/70‑1 (MQ=255) gaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:5564911/1‑71 (MQ=255) aaattCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:2987081/61‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1060063/60‑1 (MQ=38) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:2452531/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:3358243/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:1007590/1‑46 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4325223/70‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:231813/71‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:5414985/71‑1 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATa < 1:2619449/40‑1 (MQ=38) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg > 1:3880512/2‑70 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:5407114/1‑47 (MQ=255) cGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:4831018/1‑70 (MQ=255) gCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg > 1:4191845/1‑70 (MQ=39) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1666448/66‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGATAATAAGCTGCACAGGCGTCTGGACGt < 1:1691448/58‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:479102/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:949904/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg > 1:258264/1‑56 (MQ=38) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3630250/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:2563555/66‑1 (MQ=39) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:1180948/1‑71 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTgt < 1:2411341/51‑2 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1755055/71‑1 (MQ=255) caGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3084127/50‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacg < 1:2897789/71‑1 (MQ=255) gCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:2762749/1‑71 (MQ=255) cgaTCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacgac > 1:4682176/3‑71 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:93979/61‑1 (MQ=38) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:153989/65‑1 (MQ=39) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:1723496/65‑1 (MQ=39) cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:4896869/70‑1 (MQ=255) aTCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg < 1:2915520/52‑1 (MQ=255) aTCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:2484164/1‑70 (MQ=255) aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:3663167/1‑70 (MQ=255) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt < 1:4398494/41‑1 (MQ=25) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt < 1:5121478/41‑1 (MQ=25) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:3170285/1‑69 (MQ=39) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:3384762/65‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1304847/65‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt < 1:1212738/41‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt < 1:629880/41‑1 (MQ=255) ccGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa > 1:2045651/1‑61 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:4846693/55‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:4957015/61‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc > 1:3331895/1‑70 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc > 1:3945071/1‑70 (MQ=255) cGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:349716/60‑1 (MQ=255) cGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:2216808/60‑1 (MQ=255) cGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:3812282/1‑70 (MQ=38) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt > 1:1119557/1‑41 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:5029932/1‑64 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:85915/1‑64 (MQ=255) gAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:1950028/59‑1 (MQ=255) aaaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:1000416/1‑71 (MQ=255) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:716916/1‑69 (MQ=39) | GAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATA > minE/217901‑218037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |