| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,466 | C→G | 15.6% | A201A (GCG→GCC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,466 | 0 | C | G | 15.6% | 230.3 / 31.4 | 96 | A201A (GCG→GCC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (39/42); new base G (6/9); total (45/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
CAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGT > minE/1015398‑1015536 | cAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGc > 1:4211564/1‑71 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt > 1:5261989/1‑71 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt < 1:3999617/71‑1 (MQ=255) gTACCGAGGGTACACATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGATTTGGTTTc > 1:5678796/1‑71 (MQ=255) gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:3458471/1‑71 (MQ=255) gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:3451956/1‑71 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:2520491/1‑70 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt < 1:2068252/71‑1 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt < 1:2061669/71‑1 (MQ=255) aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:1410421/1‑69 (MQ=255) gTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag < 1:3478245/71‑1 (MQ=255) gTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa > 1:1316827/1‑68 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTgg < 1:2885404/57‑1 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:1084939/71‑1 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:3181154/71‑1 (MQ=255) cgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGAt < 1:4789657/70‑1 (MQ=255) cgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa > 1:5430970/1‑71 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc < 1:2189701/70‑1 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:377443/1‑71 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:5617337/1‑71 (MQ=255) ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTg < 1:3261203/48‑1 (MQ=255) ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGt < 1:4397292/50‑1 (MQ=255) ttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtttt > 1:399374/1‑71 (MQ=255) ttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTAGTAGAGATACGttt > 1:3581867/1‑68 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:5182584/1‑57 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:466936/1‑57 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:5276588/1‑57 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt > 1:3010719/1‑70 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCATGGCTTTGGTTTCCTGGTAGAGAt < 1:4334658/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:460578/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:2530501/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:2717369/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:5227161/63‑1 (MQ=255) tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc < 1:5426159/65‑1 (MQ=255) tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc < 1:4694561/65‑1 (MQ=255) ctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt < 1:4258569/68‑1 (MQ=255) tcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag > 1:4370668/1‑59 (MQ=255) cAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:105406/1‑43 (MQ=255) aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:3086951/68‑1 (MQ=255) aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:3157312/68‑1 (MQ=255) aaCCAGGCAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:5590001/68‑1 (MQ=255) ggTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa < 1:1424968/47‑1 (MQ=255) gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAac < 1:1417246/71‑1 (MQ=255) gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAac < 1:4787093/71‑1 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCt > 1:2897564/1‑42 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCt > 1:2734046/1‑42 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGcc > 1:625148/1‑65 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAacca > 1:3940664/1‑71 (MQ=255) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg < 1:4766448/58‑1 (MQ=255) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac < 1:3980521/71‑1 (MQ=255) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac < 1:4047212/71‑1 (MQ=255) tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa > 1:1575859/1‑50 (MQ=255) tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa > 1:5466074/1‑50 (MQ=255) tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa > 1:3440364/1‑50 (MQ=255) ttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATAAGTTTTGttgg > 1:2718277/1‑58 (MQ=255) ttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccacc < 1:3273283/70‑1 (MQ=255) tttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccgc > 1:5472185/1‑69 (MQ=255) tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:4008520/52‑1 (MQ=255) tCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGca > 1:2195172/1‑58 (MQ=255) tCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGc < 1:4831137/58‑1 (MQ=255) cAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt < 1:3567305/70‑1 (MQ=255) aGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCtt < 1:1191864/70‑1 (MQ=255) aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACg < 1:3921417/46‑1 (MQ=255) aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg > 1:4799489/1‑58 (MQ=255) aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg > 1:3458132/1‑58 (MQ=255) gTTTCCCCAGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTg > 1:1338241/1‑71 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGa < 1:5494009/55‑1 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc < 1:3885495/70‑1 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:1573830/1‑70 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc < 1:3608082/70‑1 (MQ=255) cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:862705/70‑1 (MQ=255) cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:1886545/70‑1 (MQ=255) ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa < 1:668128/71‑1 (MQ=255) gCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg > 1:3268327/1‑48 (MQ=255) gCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg > 1:1130160/1‑48 (MQ=255) gTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:2286384/1‑38 (MQ=255) tCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg < 1:5152596/45‑1 (MQ=255) tCAGTAACGCTTTGGTTTCCTAATAGAGATACGTCTTg < 1:594511/38‑1 (MQ=255) cAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg < 1:1611392/44‑1 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTgg > 1:247607/1‑58 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:1553698/1‑71 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:3129290/1‑71 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:5405961/1‑71 (MQ=255) gTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGttt < 1:2255237/38‑1 (MQ=255) gTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAt < 1:3288018/71‑1 (MQ=255) tAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg < 1:1165737/41‑1 (MQ=255) tAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:1364505/50‑1 (MQ=255) tAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:629610/50‑1 (MQ=255) tAACGCTTTGGGTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:1187300/50‑1 (MQ=255) aaCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGaa > 1:5173167/1‑42 (MQ=255) aaCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:1484955/49‑1 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTgg > 1:2317734/1‑54 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa > 1:4311400/1‑57 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg > 1:2861294/1‑71 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:2210864/71‑1 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg > 1:5656091/1‑71 (MQ=255) cGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt < 1:523433/71‑1 (MQ=255) | CAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGT > minE/1015398‑1015536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |