| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,508 | A→G | 26.9% | G187G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,508 | 0 | A | G | 26.9% | 167.6 / 69.6 | 110 | G187G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (37/43); new base G (13/16); total (50/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 121 total aligned reads displayed.
AATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGG > minE/1015438‑1015576 | aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAacca > 1:3940664/1‑71 (MQ=255) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac < 1:4047212/71‑1 (MQ=255) aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac < 1:3980521/71‑1 (MQ=255) ttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccacc < 1:3273283/70‑1 (MQ=255) tttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccgc > 1:5472185/1‑69 (MQ=255) cAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt < 1:3567305/70‑1 (MQ=255) aGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCtt < 1:1191864/70‑1 (MQ=255) gTTTCCCCAGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTg > 1:1338241/1‑71 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc < 1:3885495/70‑1 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:1573830/1‑70 (MQ=255) cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:862705/70‑1 (MQ=255) cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:1886545/70‑1 (MQ=255) ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa < 1:668128/71‑1 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTgg > 1:247607/1‑58 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:5405961/1‑71 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:3129290/1‑71 (MQ=255) gTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAt < 1:3288018/71‑1 (MQ=255) tAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:629610/50‑1 (MQ=255) tAACGCTTTGGGTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:1187300/50‑1 (MQ=255) aaCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac < 1:1484955/49‑1 (MQ=255) gggCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg > 1:3761804/3‑71 (MQ=255) gggCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg > 1:4443382/3‑71 (MQ=255) gggCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg < 1:289407/69‑1 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTgg > 1:2317734/1‑54 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa > 1:4311400/1‑57 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:2210864/71‑1 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg > 1:2861294/1‑71 (MQ=255) cGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt < 1:523433/71‑1 (MQ=255) gCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTa < 1:1073069/71‑1 (MQ=255) gCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTa < 1:2198266/71‑1 (MQ=255) cTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCttt > 1:1305058/1‑49 (MQ=255) cTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTac > 1:279029/1‑70 (MQ=255) cTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTac > 1:4437263/1‑70 (MQ=255) cTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTAt < 1:2561958/71‑1 (MQ=255) tttGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg < 1:2037477/67‑1 (MQ=255) tGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtc < 1:4594302/70‑1 (MQ=255) tGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtc < 1:1782442/70‑1 (MQ=255) tGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtc < 1:1224949/70‑1 (MQ=255) tGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCtt < 1:5695635/45‑1 (MQ=255) tGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtc < 1:1586922/70‑1 (MQ=255) tGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGAGATACAGAGGAATTTGTATtc < 1:1905209/70‑1 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc < 1:288495/48‑1 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc < 1:4533583/48‑1 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:422688/1‑71 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:3400818/1‑71 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcg > 1:1547230/1‑70 (MQ=255) gTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt < 1:4363897/70‑1 (MQ=255) tttCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGAt < 1:4619618/49‑1 (MQ=255) tttCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGAt < 1:5346723/49‑1 (MQ=255) tttCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:5251844/1‑58 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGc < 1:3300920/44‑1 (MQ=9) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc > 1:2245741/1‑70 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc < 1:3758264/70‑1 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCC‑CCGCCTTTCGCAACGCAAAGGaa < 1:1819799/55‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa > 1:921680/1‑46 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa > 1:5035740/1‑46 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATAc > 1:3922263/1‑49 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgtc < 1:3839973/68‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGc > 1:2995995/1‑70 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGc < 1:4641737/70‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTAGCCGAACCACCACCTTTGGCGa > 1:638196/1‑46 (MQ=255) ccTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCa > 1:3948516/1‑71 (MQ=255) gATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:1502902/56‑1 (MQ=255) gATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:1344612/56‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCTATc < 1:2453718/68‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc < 1:184854/68‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATc < 1:4116209/69‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATc < 1:1020268/69‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa < 1:3230301/71‑1 (MQ=255) tAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:1283257/1‑67 (MQ=255) tAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAAc > 1:1370218/1‑71 (MQ=255) aTACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCa > 1:2072584/1‑70 (MQ=255) tACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTa < 1:896968/50‑1 (MQ=255) tACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCAATCAACGGCAt > 1:2007269/1‑70 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatcg > 1:791211/1‑56 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatcg > 1:2748907/1‑56 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATcta > 1:2418078/1‑61 (MQ=255) aCGTCTTGTTTGCCGAACCACCACCTTTGGCGATACaga > 1:1900631/1‑39 (MQ=255) cGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTaca < 1:4390952/71‑3 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:5538074/45‑1 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:1648357/45‑1 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:5427927/45‑1 (MQ=255) cTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAt < 1:1160647/40‑1 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTAt < 1:1750528/45‑1 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa > 1:5291368/1‑65 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:2019427/40‑1 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa > 1:4382572/1‑56 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa > 1:4162793/1‑56 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:3977140/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:1592012/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:1429455/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:4485693/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgat > 1:4978529/1‑71 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgat > 1:360859/1‑71 (MQ=255) tGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt > 1:2529448/1‑38 (MQ=255) tGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt > 1:4518594/1‑38 (MQ=255) cGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcg < 1:4692434/71‑1 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGAATTTGTATtcg > 1:3157211/1‑38 (MQ=255) accaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTg < 1:725818/71‑1 (MQ=255) ccaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTgg < 1:4156516/71‑1 (MQ=255) | AATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGG > minE/1015438‑1015576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |