| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,996 | A→T | 79.8% | intergenic (‑88/‑18) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,996 | 0 | A | T | 79.8% | 460.4 / 105.1 | 207 | intergenic (‑88/‑18) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (18/24); new base T (89/76); total (107/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 211 total aligned reads displayed.
CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217928‑218065 | gATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCg > 1:134022/1‑71 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt > 1:4721210/1‑71 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGGAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:2536454/69‑1 (MQ=255) aTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:4158700/69‑1 (MQ=255) tAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt < 1:1294206/70‑1 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:2028959/70‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:4357549/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:1187589/71‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1464334/3‑71 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1425037/68‑1 (MQ=255) agaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:4600385/70‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:2818678/64‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4857679/71‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:3358243/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat > 1:2452531/1‑71 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:296888/1‑70 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:4839950/1‑70 (MQ=255) ttCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCTCAGGCGTCTGGACGTGGTaatg < 1:2313760/69‑1 (MQ=255) cGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:3005613/1‑70 (MQ=255) gCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg > 1:4191845/1‑70 (MQ=39) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1666448/66‑1 (MQ=255) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1526480/66‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:2614983/71‑1 (MQ=39) ccACAGCAATCCGAAACCACTGGCACGTGGATAATAAGCTGCACAGGCGTCTGGACGt < 1:1691448/58‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:479102/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3630250/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:2563555/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3734292/66‑1 (MQ=39) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTgt < 1:2411341/51‑2 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac > 1:2921521/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1755055/71‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTc < 1:3297961/47‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacg < 1:2897789/71‑1 (MQ=255) aGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga > 1:5182723/1‑66 (MQ=39) gCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:5384363/1‑71 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:153989/65‑1 (MQ=39) cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:4896869/70‑1 (MQ=255) aTCCGAAATCTCTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGa < 1:2041284/47‑1 (MQ=255) aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:3663167/1‑70 (MQ=255) aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:5408078/66‑1 (MQ=255) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt < 1:5121478/41‑1 (MQ=25) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:3170285/1‑69 (MQ=39) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:3384762/65‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:4846693/55‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:4957015/61‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:5526294/61‑1 (MQ=255) cGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:2216808/60‑1 (MQ=255) cGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:349716/60‑1 (MQ=255) cGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:3812282/1‑70 (MQ=38) cGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:916147/1‑70 (MQ=38) cGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:3648158/1‑70 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCt > 1:1119557/1‑41 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:85915/1‑64 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:5029932/1‑64 (MQ=255) aaaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:1000416/1‑71 (MQ=255) atcACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAAt > 1:4553976/3‑70 (MQ=255) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:716916/1‑69 (MQ=39) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAAt > 1:4678397/1‑70 (MQ=255) aCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATa < 1:1218100/71‑1 (MQ=255) cACTGCCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgtt < 1:3148034/44‑3 (MQ=37) aCTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:1612584/1‑70 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:1143779/1‑70 (MQ=255) cTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatga > 1:3975418/1‑47 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:68454/54‑1 (MQ=255) cGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATa < 1:3341300/61‑1 (MQ=255) gTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaa > 1:5445949/1‑71 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:2480457/38‑1 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1860410/38‑1 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:394128/37‑1 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGa < 1:4260102/68‑1 (MQ=255) ggAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4313151/36‑1 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:2136022/1‑68 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:2787229/1‑68 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:2582552/1‑68 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACg > 1:441543/1‑70 (MQ=255) gagaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg < 1:890734/70‑1 (MQ=255) agaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:2707129/1‑46 (MQ=255) aaTAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:2337351/37‑1 (MQ=255) aaTAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg < 1:585044/37‑1 (MQ=255) aaTAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:3238460/39‑1 (MQ=255) aTAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:3883483/1‑43 (MQ=255) aTAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:3821537/1‑43 (MQ=255) aTAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAga > 1:5509808/1‑40 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa > 1:832923/1‑37 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:1812377/1‑42 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAga > 1:4487658/1‑39 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaa > 1:3849266/1‑53 (MQ=255) tAAGCTGCACAGTCGTCTGGACGTGGTAATGAATGAAgac < 1:942508/40‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:2228353/1‑39 (MQ=255) tAAGCAGCACTGGAGTCTGGACGTGGTAATGAATGAAga > 1:4099393/1‑39 (MQ=37) aGCTGCACTGGCGTCTGGGCGTGGTAATGAATGAAGACGACTGCAAAAt > 1:3687921/1‑49 (MQ=38) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:3080510/1‑40 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:5621552/1‑52 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:3116917/1‑52 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaa > 1:2125525/1‑51 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaa > 1:2092121/1‑51 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:1722551/1‑61 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:103484/1‑61 (MQ=255) aGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaaa > 1:2262793/1‑61 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATa < 1:5343441/42‑1 (MQ=255) | CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217928‑218065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |