| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,520 | G→A | 22.6% | I183I (ATC→ATT) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,520 | 0 | G | A | 22.6% | 216.5 / 58.1 | 115 | I183I (ATC→ATT) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (44/45); new base A (12/14); total (56/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGT > minE/1015452‑1015585 | cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:1886545/70‑1 (MQ=255)cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:862705/70‑1 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:3129290/1‑71 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:5405961/1‑71 (MQ=255) gTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAt < 1:3288018/71‑1 (MQ=255) gggCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg > 1:3761804/3‑71 (MQ=255) gggCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg < 1:289407/69‑1 (MQ=255) gggCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg > 1:4443382/3‑71 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa > 1:4311400/1‑57 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg > 1:2861294/1‑71 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:2210864/71‑1 (MQ=255) aCGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg > 1:5656091/1‑71 (MQ=255) cGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt < 1:523433/71‑1 (MQ=255) gCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTa < 1:2198266/71‑1 (MQ=255) gCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTa < 1:1073069/71‑1 (MQ=255) cTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTac > 1:4437263/1‑70 (MQ=255) cTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTac > 1:279029/1‑70 (MQ=255) cTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTAt < 1:2561958/71‑1 (MQ=255) tttGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg < 1:2037477/67‑1 (MQ=255) tttGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTg < 1:4939290/67‑1 (MQ=255) tGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtc < 1:4594302/70‑1 (MQ=255) tGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtc < 1:1224949/70‑1 (MQ=255) tGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtc < 1:1586922/70‑1 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:3400818/1‑71 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:422688/1‑71 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:5297930/1‑71 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcg > 1:1547230/1‑70 (MQ=255) gTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt < 1:4363897/70‑1 (MQ=255) tttCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:5251844/1‑58 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc > 1:2245741/1‑70 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCC‑CCGCCTTTCGCAACGCAAAGGaa < 1:1819799/55‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATAc > 1:3922263/1‑49 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgtc < 1:3839973/68‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGc < 1:4641737/70‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGc > 1:2995995/1‑70 (MQ=255) tCCTGATAGAGATACGTCTTGTTAGCCGAACCACCACCTTTGGCGa > 1:638196/1‑46 (MQ=255) cTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGt < 1:262458/59‑1 (MQ=255) cTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGt < 1:4192742/59‑1 (MQ=255) tggtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:3978994/4‑70 (MQ=255) tggtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:1893027/4‑70 (MQ=255) gATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:1502902/56‑1 (MQ=255) gATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:1344612/56‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCTATc < 1:2453718/68‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc < 1:1750586/68‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc < 1:184854/68‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATc < 1:4116209/69‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATc < 1:1020268/69‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTAGTCGCCATc < 1:4432689/69‑1 (MQ=255) tAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:1283257/1‑67 (MQ=255) tAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAAc > 1:1370218/1‑71 (MQ=255) tACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTa < 1:896968/50‑1 (MQ=255) tACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCAATCAACGGCAt > 1:2007269/1‑70 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatcg > 1:791211/1‑56 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatcg > 1:2748907/1‑56 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATcta > 1:2418078/1‑61 (MQ=255) aCGTCTTGTTTGCCGAACCACCACCTTTGGCGATACaga > 1:2055945/1‑39 (MQ=255) aCGTCTTGTTTGCCGAACCACCACCTTTGGCGATACaga > 1:1900631/1‑39 (MQ=255) cGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTaca < 1:4390952/71‑3 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:1648357/45‑1 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:5427927/45‑1 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:5538074/45‑1 (MQ=255) cTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAt < 1:1160647/40‑1 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTAt < 1:1750528/45‑1 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa > 1:5291368/1‑65 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAga < 1:1196073/69‑1 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:2019427/40‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:3977140/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:1592012/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:3911620/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAAttt < 1:1429455/38‑1 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgat > 1:360859/1‑71 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgat > 1:4978529/1‑71 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcga > 1:453718/1‑70 (MQ=255) tGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt > 1:4518594/1‑38 (MQ=255) tGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt > 1:2529448/1‑38 (MQ=255) tGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt > 1:3242740/1‑38 (MQ=255) cGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcg < 1:4692434/71‑1 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGAATTTGTATtcg > 1:3157211/1‑38 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgatc < 1:743428/65‑1 (MQ=255) accaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTg < 1:725818/71‑1 (MQ=255) ccaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTgg < 1:4156516/71‑1 (MQ=255) accTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCt < 1:2720264/59‑1 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGc > 1:2795932/1‑44 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4584255/47‑1 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4458752/47‑1 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAtt < 1:4524809/71‑1 (MQ=255) cTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgat > 1:3159724/1‑51 (MQ=255) cTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTg < 1:3775936/71‑1 (MQ=255) tttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTgg > 1:2072133/1‑71 (MQ=255) tttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTgg > 1:3325390/1‑71 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa > 1:2488213/1‑37 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAga > 1:4013716/1‑48 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAg < 1:4629740/47‑1 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGt > 1:4099552/1‑71 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGt > 1:1891320/1‑71 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGt > 1:226183/1‑71 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:5315601/39‑1 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4176970/39‑1 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4971077/39‑1 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:2108055/39‑1 (MQ=255) | CCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGT > minE/1015452‑1015585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |