| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,541 | G→A | 17.9% | D176D (GAC→GAT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,541 | 0 | G | A | 17.9% | 195.4 / 31.5 | 89 | D176D (GAC→GAT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (37/36); new base A (7/9); total (44/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATAT > minE/1015472‑1015611 | ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:5297930/1‑71 (MQ=255)ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:3400818/1‑71 (MQ=255)ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt > 1:422688/1‑71 (MQ=255)ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcg > 1:1547230/1‑70 (MQ=255) gTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgt < 1:4363897/70‑1 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc > 1:2245741/1‑70 (MQ=255) tCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc < 1:3758264/70‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcgtc < 1:3839973/68‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGc < 1:4641737/70‑1 (MQ=255) tCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGc > 1:2995995/1‑70 (MQ=255) ccTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCa > 1:3948516/1‑71 (MQ=255) tggtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:1893027/4‑70 (MQ=255) tggtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:3978994/4‑70 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCTATc < 1:2453718/68‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc < 1:1750586/68‑1 (MQ=255) gtAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc < 1:184854/68‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATc < 1:4116209/69‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATc < 1:1020268/69‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa < 1:3230301/71‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTAGTCGCCATc < 1:4432689/69‑1 (MQ=255) tAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt > 1:1283257/1‑67 (MQ=255) tAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAAc > 1:1370218/1‑71 (MQ=255) aTACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCa > 1:2072584/1‑70 (MQ=255) aTACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCa > 1:2886802/1‑70 (MQ=255) tACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCAATCAACGGCAt > 1:2007269/1‑70 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatcg > 1:791211/1‑56 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatcg > 1:2748907/1‑56 (MQ=255) aCGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATcta > 1:2418078/1‑61 (MQ=255) cGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTaca < 1:4390952/71‑3 (MQ=255) ttttgttgGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg > 1:2328173/3‑70 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa > 1:5291368/1‑65 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAga < 1:1196073/69‑1 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa > 1:4162793/1‑56 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa > 1:4382572/1‑56 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgat > 1:360859/1‑71 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgat > 1:4978529/1‑71 (MQ=255) tttGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcga > 1:453718/1‑70 (MQ=255) cGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcg < 1:4692434/71‑1 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGAATTTGTATtcg > 1:3157211/1‑38 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcgatc < 1:743428/65‑1 (MQ=255) accaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTg < 1:725818/71‑1 (MQ=255) ccaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTgg < 1:4156516/71‑1 (MQ=255) accTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCt < 1:2720264/59‑1 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGc > 1:2795932/1‑44 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4584255/47‑1 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4458752/47‑1 (MQ=255) ccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAtt < 1:4524809/71‑1 (MQ=255) ccTTTCGCGATACAGAGGAATTTGTATTCGTCGCCa > 1:1318878/1‑36 (MQ=255) cTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgat > 1:2622213/1‑51 (MQ=255) cTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgat > 1:3159724/1‑51 (MQ=255) cTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTg < 1:3775936/71‑1 (MQ=255) tttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTgg > 1:2072133/1‑71 (MQ=255) tttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTgg > 1:3325390/1‑71 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa > 1:2488213/1‑37 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAga > 1:4013716/1‑48 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAg < 1:4629740/47‑1 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGt > 1:1891320/1‑71 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGt > 1:4099552/1‑71 (MQ=255) ttGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGt > 1:226183/1‑71 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:2108055/39‑1 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4176970/39‑1 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:4971077/39‑1 (MQ=255) gATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:5315601/39‑1 (MQ=255) aTACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTg < 1:1310120/63‑1 (MQ=255) tACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcgc < 1:5610401/52‑1 (MQ=255) tACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTa > 1:4344834/1‑71 (MQ=255) tACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTa > 1:3207396/1‑71 (MQ=255) caaagGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGATTTGCGCAGGCAGGTTAGTGCCGGTGtt < 1:4681343/68‑1 (MQ=255) caaagGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGATTTGCGCAGGCAGGTTAGTGCCGGTGtt < 1:1091046/68‑1 (MQ=255) caaagGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGATTTGCGCAGGCAGGTTAGTGCCGGTGtt < 1:1514313/68‑1 (MQ=255) cAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTAtt < 1:5620944/71‑1 (MQ=255) cAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTAtt < 1:731829/71‑1 (MQ=255) cAGAGGAATTTGTATTCATCGCCATCAACGGCATAAAGATCGATCTGCGCTg > 1:249488/1‑52 (MQ=255) agagGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTc < 1:2360023/71‑1 (MQ=255) agGAATTTGTACTCATCGCCATCTACCGCTTACAGGTCGATTTGCGCAGGCAGGTTAGTGCCGGTGtt < 1:396478/68‑1 (MQ=255) ggAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAtt > 1:2818779/1‑55 (MQ=255) ggAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAtt > 1:4419217/1‑55 (MQ=255) ggAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAtt > 1:4584288/1‑55 (MQ=255) ggAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCAct < 1:3024302/71‑1 (MQ=255) aaTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcgc > 1:13705/1‑44 (MQ=255) aaTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcgc > 1:2143245/1‑44 (MQ=255) aaTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGa < 1:2050158/51‑1 (MQ=255) aaTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGa < 1:5270838/51‑1 (MQ=255) aaTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGa < 1:5111803/51‑1 (MQ=255) aaTTCGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcgc > 1:4555774/1‑44 (MQ=255) aTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCa < 1:554684/48‑1 (MQ=255) aTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCa < 1:5671698/48‑1 (MQ=255) aTTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCa < 1:781674/48‑1 (MQ=255) tttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCa < 1:1503289/47‑1 (MQ=255) tttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAt < 1:1423350/50‑1 (MQ=255) tttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCActtctt < 1:4432141/71‑1 (MQ=255) ttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCActtctt > 1:4243759/1‑70 (MQ=255) ttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCActtctt > 1:4250465/1‑70 (MQ=255) tGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTg > 1:2239319/1‑50 (MQ=255) tATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTtata > 1:142132/1‑71 (MQ=255) tATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTtat < 1:5629182/70‑1 (MQ=255) aTTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTtata > 1:4939793/1‑70 (MQ=255) tcgtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCAc > 1:2745223/1‑59 (MQ=255) tcgtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACa > 1:2891749/1‑70 (MQ=255) gtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACatat < 1:1752028/71‑1 (MQ=255) | GGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATAT > minE/1015472‑1015611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |