| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,030 | A→G | 73.7% | Y6C (TAC→TGC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,030 | 0 | A | G | 73.7% | 326.1 / 126.4 | 201 | Y6C (TAC→TGC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/25); new base G (98/50); total (126/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 201 total aligned reads displayed.
CCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTT > minE/217962‑218096 | ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc > 1:3945071/1‑70 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:3648158/1‑70 (MQ=255) aaaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:1000416/1‑71 (MQ=255) atcACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAAt > 1:4553976/3‑70 (MQ=255) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:716916/1‑69 (MQ=39) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAAt > 1:4678397/1‑70 (MQ=255) gTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaa > 1:5445949/1‑71 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaat > 1:3074972/1‑70 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaat > 1:4662215/1‑70 (MQ=255) tGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGa < 1:4260102/68‑1 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:3893140/1‑68 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:5649601/1‑68 (MQ=255) gagaATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACg > 1:4017662/1‑70 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:311949/1‑70 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaa > 1:1531927/1‑53 (MQ=255) tAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaa > 1:3849266/1‑53 (MQ=255) aGCTGCACTGGCGTCTGGGCGTGGTAATGAATGAAGACGACTGCAAAAt > 1:3687921/1‑49 (MQ=38) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:5621552/1‑52 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaa > 1:2125525/1‑51 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:103484/1‑61 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaatg > 1:1722551/1‑61 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGaa > 1:183671/1‑71 (MQ=255) aGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:3431688/1‑71 (MQ=255) tGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatta > 1:702751/1‑71 (MQ=255) agCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatt > 1:2921597/2‑70 (MQ=255) actggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAttt > 1:2476369/4‑71 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGTAAATGCAGCAGAATTAtttt > 1:379680/3‑71 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:2305285/3‑71 (MQ=255) caGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:261775/71‑1 (MQ=255) caGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:4452388/71‑1 (MQ=255) caGGCGGCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAgg < 1:381832/51‑1 (MQ=255) tggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGa < 1:1621040/50‑1 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaa > 1:4178891/1‑37 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:4531804/1‑71 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3619865/1‑71 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaa > 1:3002787/1‑50 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg < 1:3748484/71‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag < 1:872150/39‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatta > 1:2613107/1‑61 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:3031516/1‑70 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:4237334/1‑42 (MQ=255) ggACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGaa < 1:3307548/55‑1 (MQ=255) gACGTGGTATTGAATGAAGACGACTGCAAAATAAGAAGAGGa < 1:1100447/42‑1 (MQ=38) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:1587740/1‑67 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:4419242/1‑67 (MQ=255) tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:1214694/1‑70 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGaaa > 1:2072664/1‑39 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3224214/1‑71 (MQ=255) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:5314869/1‑71 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:5633730/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:5524303/1‑71 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2329927/1‑71 (MQ=255) tattgattgaaGACGACTGCAAAATAAGTAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca < 1:3340743/66‑1 (MQ=255) tAATGAATGAAGACGACTGCAAAATAAGAAGAGGa > 1:1373102/1‑35 (MQ=255) aatgaatgAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:90337/1‑68 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1145339/53‑1 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa > 1:1223351/1‑54 (MQ=39) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACgg > 1:2680293/1‑63 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:4311200/1‑69 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGc > 1:4188913/1‑71 (MQ=255) tgagtgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGc > 1:4580661/1‑70 (MQ=255) gaatgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt > 1:2553238/1‑50 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa > 1:580294/1‑51 (MQ=39) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:5424152/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattat > 1:4847072/1‑46 (MQ=255) ttgatgaCGACTGCAAAATAAGAAGAGGAAATgca < 1:412162/34‑1 (MQ=21) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:1299706/48‑1 (MQ=38) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:4750907/47‑1 (MQ=38) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:479685/1‑51 (MQ=39) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:5344283/1‑50 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:4403200/1‑69 (MQ=255) tgaaGACGACCGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt > 1:5295270/1‑70 (MQ=255) caaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:3349400/45‑1 (MQ=255) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:4026744/1‑70 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:4932436/1‑70 (MQ=255) cgacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa < 1:2274405/68‑1 (MQ=255) acacgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt < 1:5632639/42‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGcagcag < 1:2019559/35‑1 (MQ=25) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatt < 1:2441357/39‑1 (MQ=37) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc < 1:2969280/45‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:5099815/67‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatt < 1:2166000/39‑1 (MQ=37) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt < 1:2795275/44‑1 (MQ=38) aGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:3176870/39‑1 (MQ=255) aGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAata < 1:2389810/71‑1 (MQ=255) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:831704/49‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:3765346/48‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:2519896/48‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:3524767/55‑1 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtt > 1:450137/1‑71 (MQ=255) acgaaTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:5676934/41‑1 (MQ=25) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:408413/41‑1 (MQ=38) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:5400852/47‑1 (MQ=38) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:2262752/54‑1 (MQ=39) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:1357884/39‑1 (MQ=37) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa > 1:3481111/1‑42 (MQ=38) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2239943/71‑1 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:3239038/40‑1 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:149770/40‑1 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2872822/71‑1 (MQ=255) | CCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTT > minE/217962‑218096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |