| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,049 | C→T | 63.4% | N12N (AAC→AAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,049 | 0 | C | T | 63.4% | 177.8 / 208.5 | 213 | N12N (AAC→AAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (42/36); new base T (75/60); total (117/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
Only 100 of 215 total aligned reads displayed.
GAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGG > minE/217981‑218119 | gagaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg < 1:890734/70‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:1359225/1‑70 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:311949/1‑70 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:4570676/1‑68 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGaa > 1:183671/1‑71 (MQ=255) aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGaa > 1:4182443/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta > 1:3152724/1‑71 (MQ=255) actggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGcagcag < 1:1353206/60‑1 (MQ=255) actggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt > 1:1553993/4‑71 (MQ=255) actggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAttt > 1:2476369/4‑71 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt > 1:2305285/3‑71 (MQ=255) ggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt > 1:56813/1‑69 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:3619865/1‑71 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:671335/1‑71 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:4531804/1‑71 (MQ=255) gCGTCTGGACGTGGTAATGAATGAACACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa < 1:3796423/70‑1 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg < 1:3748484/71‑1 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatta > 1:2613107/1‑61 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:2518259/1‑70 (MQ=255) ggACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGaa < 1:3307548/55‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:4419242/1‑67 (MQ=255) tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:1214694/1‑70 (MQ=255) ggTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:3224214/1‑71 (MQ=255) gTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:5314869/1‑71 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:5633730/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2329927/1‑71 (MQ=255) tattgattgaaGACGACTGCAAAATAAGTAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca < 1:3340743/66‑1 (MQ=255) tAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa > 1:171575/1‑56 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1145339/53‑1 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATgcagca > 1:2257540/1‑42 (MQ=38) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:2440737/1‑69 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:4311200/1‑69 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:304137/1‑71 (MQ=255) ttaatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:5656797/62‑1 (MQ=255) tgaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:1942229/46‑1 (MQ=255) gaatgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt > 1:2553238/1‑50 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACgg < 1:715715/61‑1 (MQ=255) aatgaaGACGACTGCAAAATAAGAAGAGGAAATgca > 1:3494624/1‑36 (MQ=37) aatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:2615393/1‑71 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattat > 1:5437508/1‑46 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg > 1:4225505/1‑52 (MQ=255) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg < 1:4952854/51‑1 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt > 1:1028655/1‑70 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:3233097/1‑69 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAt > 1:4403200/1‑69 (MQ=255) tgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc < 1:3350386/66‑1 (MQ=255) aaGACGACTGCAAAATAAGAAGAGGAAATgcagca > 1:1258354/1‑35 (MQ=25) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:5394675/1‑70 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTc > 1:4097642/1‑46 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:4932436/1‑70 (MQ=255) cgacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa < 1:2274405/68‑1 (MQ=255) acacgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt < 1:5632639/42‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGcagcag < 1:2019559/35‑1 (MQ=25) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:2458371/44‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:4025033/43‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:4744382/43‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:4016282/48‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt < 1:2795275/44‑1 (MQ=38) aGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAata < 1:2389810/71‑1 (MQ=255) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:831704/49‑1 (MQ=38) gacgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:2987679/56‑1 (MQ=255) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:3765346/48‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtt > 1:450137/1‑71 (MQ=255) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:3847454/41‑1 (MQ=38) cgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg < 1:5622136/44‑1 (MQ=255) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:2661568/1‑71 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:149770/40‑1 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2872822/71‑1 (MQ=255) ctgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:3547216/4‑71 (MQ=255) ctgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:5377879/4‑71 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt > 1:4668912/1‑45 (MQ=255) gcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:4797169/39‑1 (MQ=38) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:404943/39‑1 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATa > 1:3190140/1‑43 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATa > 1:3687767/1‑43 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:114062/1‑50 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:4291140/1‑52 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:762677/1‑70 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGAACATTGCTATTAATATTTTGa > 1:5052829/1‑68 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg > 1:2926465/1‑38 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:5263405/1‑70 (MQ=255) aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa < 1:541146/70‑1 (MQ=255) aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:5569306/1‑45 (MQ=255) aTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATga < 1:3439115/71‑1 (MQ=255) tAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGa > 1:3659104/1‑36 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata < 1:222589/70‑3 (MQ=255) gaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAg < 1:3909241/71‑1 (MQ=255) aagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg < 1:5116099/71‑1 (MQ=255) agagGAAACGCAGCAGAATTATTTTCAGGGATACGGc < 1:2917256/37‑1 (MQ=255) gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:3475987/69‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt < 1:435695/71‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt < 1:4482819/71‑1 (MQ=255) ggAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt < 1:553203/60‑1 (MQ=255) gAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAtt > 1:4751481/1‑70 (MQ=255) gAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc < 1:930754/71‑1 (MQ=255) aaCGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTc > 1:2723863/1‑69 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc < 1:1627686/69‑1 (MQ=255) aCGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg < 1:4908362/53‑1 (MQ=38) aCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:3705333/41‑1 (MQ=255) cGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg > 1:4587884/1‑71 (MQ=255) | GAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGG > minE/217981‑218119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |