| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,052 | A→C | 9.4% | A13A (GCA→GCC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,052 | 0 | A | C | 9.4% | 494.9 / 44.3 | 203 | A13A (GCA→GCC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (100/84); new base C (11/8); total (111/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01 | |||||||||||
Only 100 of 230 total aligned reads displayed.
AATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCA > minE/217984‑218121 | aGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGaa > 1:4182443/1‑71 (MQ=255) aGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:3431688/1‑71 (MQ=255) tGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatta > 1:3152724/1‑71 (MQ=255) actggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt > 1:1553993/4‑71 (MQ=255) ctggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGTAAATGCAGCAGAATTAtttt > 1:379680/3‑71 (MQ=255) caGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:261775/71‑1 (MQ=255) ggCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt > 1:56813/1‑69 (MQ=255) gCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAg > 1:4531804/1‑71 (MQ=255) cGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg < 1:3748484/71‑1 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATa > 1:3643005/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:4332641/58‑1 (MQ=255) ggACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGaa < 1:3307548/55‑1 (MQ=255) gACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:4419242/1‑67 (MQ=255) tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:1214694/1‑70 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:5633730/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:3464882/1‑57 (MQ=255) gTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAt > 1:2329927/1‑71 (MQ=255) tattgattgaaGACGACTGCAAAATAAGTAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca < 1:3340743/66‑1 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:1145339/53‑1 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCATGGATACGGCACATTg > 1:2279801/1‑71 (MQ=255) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATgcagca > 1:2257540/1‑42 (MQ=38) atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc > 1:865715/1‑53 (MQ=39) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:304137/1‑71 (MQ=255) ttaatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:5656797/62‑1 (MQ=255) tgagtgaaGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGc > 1:4580661/1‑70 (MQ=255) gaatgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTa > 1:806986/1‑71 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattatt > 1:1819178/1‑48 (MQ=255) gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACgg < 1:715715/61‑1 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAattat > 1:5437508/1‑46 (MQ=255) aatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg > 1:4225505/1‑52 (MQ=255) ttgatgaCGACTGCAAAATAAGAAGAGGAAATgca < 1:412162/34‑1 (MQ=21) atgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:1299706/48‑1 (MQ=38) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:5344283/1‑50 (MQ=255) atgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg < 1:4952854/51‑1 (MQ=255) tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt > 1:1028655/1‑70 (MQ=255) tgaaGACGACCGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt > 1:5295270/1‑70 (MQ=255) gaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAat < 1:3020020/40‑1 (MQ=37) aaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:5394675/1‑70 (MQ=255) aaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa > 1:4932436/1‑70 (MQ=255) acacgacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt < 1:5632639/42‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAatt < 1:2441357/39‑1 (MQ=37) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:2458371/44‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:4744382/43‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:4025033/43‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTc < 1:2969280/45‑1 (MQ=38) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATa < 1:3585513/52‑1 (MQ=39) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:5099815/67‑1 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAatt < 1:2166000/39‑1 (MQ=37) aGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTAtttt < 1:2795275/44‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:3913668/42‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa < 1:2519896/48‑1 (MQ=38) acgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtt > 1:450137/1‑71 (MQ=255) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAtttt < 1:3847454/41‑1 (MQ=38) cgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAg < 1:5622136/44‑1 (MQ=255) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTAttt < 1:1357884/39‑1 (MQ=37) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCa > 1:3481111/1‑42 (MQ=38) gacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:2661568/1‑71 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:3239038/40‑1 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt < 1:149770/40‑1 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt < 1:2872822/71‑1 (MQ=255) acTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGcac < 1:3681221/53‑1 (MQ=255) ctgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:3547216/4‑71 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGAt > 1:4668912/1‑45 (MQ=255) gcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:2608857/39‑1 (MQ=38) gcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:4797169/39‑1 (MQ=38) gcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:1955574/2‑70 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAgg < 1:404943/39‑1 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATa > 1:3190140/1‑43 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGcaca > 1:114062/1‑50 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:4291140/1‑52 (MQ=255) cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGAACATTGCTATTAATATTTTGa > 1:5052829/1‑68 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:3226845/1‑37 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg > 1:2493940/1‑70 (MQ=255) cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg < 1:2613971/70‑1 (MQ=255) aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa < 1:541146/70‑1 (MQ=255) aaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGca < 1:1883687/45‑1 (MQ=255) aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGca > 1:5569306/1‑45 (MQ=255) tAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa > 1:379098/1‑67 (MQ=255) tAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATga > 1:4359183/1‑70 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata < 1:222589/70‑3 (MQ=255) agagGAAACGCAGCAGAATTATTTTCAGGGATACGGc < 1:2917256/37‑1 (MQ=255) agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt < 1:4482819/71‑1 (MQ=255) ggAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt < 1:553203/60‑1 (MQ=255) gAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAtt > 1:4751481/1‑70 (MQ=255) gAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc < 1:930754/71‑1 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc > 1:2406640/1‑70 (MQ=255) aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCa < 1:5170594/71‑1 (MQ=255) aatgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc > 1:682518/4‑69 (MQ=255) aatgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCa < 1:1898906/67‑1 (MQ=255) aaCGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTc > 1:2723863/1‑69 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc < 1:1627686/69‑1 (MQ=255) atgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATga < 1:669350/56‑1 (MQ=255) aCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt < 1:3705333/41‑1 (MQ=255) tgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAgg < 1:3142753/70‑1 (MQ=255) tgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAgg < 1:3695564/70‑1 (MQ=255) gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATaa > 1:2413925/1‑59 (MQ=255) gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATaa > 1:136121/1‑59 (MQ=255) cagcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAgg > 1:4892314/1‑69 (MQ=255) cagcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg > 1:2306825/1‑69 (MQ=255) agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:461686/1‑47 (MQ=255) | AATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCA > minE/217984‑218121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |