Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,015,795 G→T 17.4% R92S (CGT→AGT) ‡ fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,015,7950GT17.4% 277.6 / 31.3 103R92S (CGT→AGT) ‡fumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (62/23);  new base T (8/10);  total (70/33)
Fisher's exact test for biased strand distribution p-value = 2.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 103 total aligned reads displayed.

CAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCT  >  minE/1015729‑1015863
                                                                  |                                                                    
caaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg                                                                  <  1:1928952/71‑1 (MQ=255)
 aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg                                                                  >  1:3286898/1‑70 (MQ=255)
   taatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt                                                               >  1:2007616/1‑71 (MQ=255)
     atCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTg                                                             <  1:791435/71‑1 (MQ=255)
     atCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTg                                                             <  1:3132523/71‑1 (MQ=255)
             gCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca                                                     >  1:4192488/1‑71 (MQ=255)
                ggTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCcac                                                    >  1:1102281/1‑69 (MQ=255)
                  tATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACata                                                 <  1:3301211/70‑1 (MQ=255)
                   aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt                                               >  1:3329695/1‑71 (MQ=255)
                   aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt                                               >  1:5109385/1‑71 (MQ=255)
                    tCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCcacg                                                   <  1:5676077/66‑2 (MQ=255)
                    tCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac                                                <  1:4853166/69‑2 (MQ=255)
                    tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGc                                                            <  1:1596908/57‑1 (MQ=255)
                    tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca                                                     <  1:1046048/64‑1 (MQ=255)
                    tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt                                               >  1:542707/1‑70 (MQ=255)
                    tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt                                               >  1:4150612/1‑70 (MQ=255)
                      cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTa                                             <  1:3355582/70‑1 (MQ=255)
                      cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTAt                                            >  1:3885883/1‑71 (MQ=255)
                      cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTAt                                            >  1:1937606/1‑71 (MQ=255)
                       tggcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTc                                           >  1:3719848/4‑71 (MQ=255)
                        gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg                                                             >  1:3165474/1‑52 (MQ=255)
                        gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa                                                           >  1:2935923/1‑54 (MQ=255)
                        gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa                                                           >  1:4178052/1‑54 (MQ=255)
                        gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa                                                           >  1:4388660/1‑54 (MQ=255)
                         gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcgt                                         >  1:198594/2‑69 (MQ=255)
                         gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcg                                          <  1:3760770/69‑2 (MQ=255)
                         aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATc                                           <  1:1445599/69‑1 (MQ=255)
                         aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt                                         >  1:1035501/1‑71 (MQ=255)
                          cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                        >  1:5096043/1‑71 (MQ=255)
                          cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                        <  1:4048499/71‑1 (MQ=255)
                             gtcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTc                                     <  1:184778/68‑1 (MQ=255)
                              tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg                                    <  1:800685/69‑1 (MQ=255)
                              tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg                                    <  1:4983230/69‑1 (MQ=255)
                                ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                   <  1:1965448/70‑1 (MQ=255)
                                ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                  >  1:3020013/1‑71 (MQ=255)
                                  cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg                                >  1:389951/1‑71 (MQ=255)
                                  cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg                                >  1:1444056/1‑71 (MQ=255)
                                    gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                              >  1:4096225/1‑71 (MQ=255)
                                    gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                              >  1:4326027/1‑71 (MQ=255)
                                    gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                              >  1:978119/1‑71 (MQ=255)
                                    gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                              >  1:321512/1‑71 (MQ=255)
                                    gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                               >  1:334766/1‑70 (MQ=255)
                                     aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa                                                                <  1:2324461/36‑1 (MQ=255)
                                     aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa                                                                <  1:2351181/36‑1 (MQ=255)
                                     aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCt                             <  1:2604459/71‑1 (MQ=255)
                                      aCGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt                                  >  1:3612019/1‑65 (MQ=255)
                                      aCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa                                                                <  1:2073473/35‑1 (MQ=255)
                                       cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt                                                               >  1:1969188/1‑35 (MQ=255)
                                       cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt                                                               >  1:3904525/1‑35 (MQ=255)
                                       cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc                           >  1:4245253/1‑71 (MQ=255)
                                       cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc                           >  1:2402841/1‑71 (MQ=255)
                                        gCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGtttt                                      <  1:4648872/59‑1 (MQ=255)
                                         ccTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg                          >  1:3510957/1‑68 (MQ=255)
                                           tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc                                     <  1:1010751/57‑1 (MQ=255)
                                           tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc                                     <  1:1200733/57‑1 (MQ=255)
                                            ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCcaca                                                   >  1:1137746/1‑42 (MQ=255)
                                            ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCcaca                                                   >  1:1340184/1‑42 (MQ=255)
                                            ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt                       >  1:2318724/1‑70 (MQ=255)
                                            ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGt                       >  1:5195157/1‑70 (MQ=255)
                                             tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                   >  1:3601394/1‑57 (MQ=255)
                                             tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                   >  1:3277650/1‑57 (MQ=255)
                                             tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                   >  1:2722800/1‑57 (MQ=255)
                                                 cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg                   >  1:5304385/1‑69 (MQ=255)
                                                 cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg                   >  1:5350317/1‑69 (MQ=255)
                                                 cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg                   >  1:53878/1‑69 (MQ=255)
                                                  gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac                                                >  1:3582023/1‑38 (MQ=12)
                                                  gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac                                                >  1:134986/1‑38 (MQ=12)
                                                  gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac                                                >  1:3307507/1‑38 (MQ=12)
                                                   cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa               <  1:3181149/71‑1 (MQ=255)
                                                    gATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                        <  1:3794902/45‑1 (MQ=255)
                                                     atttCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAAt             <  1:5408500/68‑1 (MQ=255)
                                                     aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtttt                                      >  1:3358815/1‑46 (MQ=255)
                                                     aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt                                         <  1:1164286/43‑1 (MQ=255)
                                                      tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg                         >  1:4340196/1‑58 (MQ=255)
                                                      tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg            <  1:5305721/71‑1 (MQ=255)
                                                       gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGc                  >  1:3968666/1‑64 (MQ=255)
                                                       gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt           >  1:1802020/1‑71 (MQ=255)
                                                       gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt           >  1:780844/1‑71 (MQ=255)
                                                          ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                               >  1:4184880/1‑48 (MQ=255)
                                                          ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                               >  1:3472034/1‑48 (MQ=255)
                                                              ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTc                                     <  1:1534111/38‑1 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                    >  1:806016/1‑37 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                    >  1:3437760/1‑37 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                    >  1:3471870/1‑37 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                   >  1:3403599/1‑38 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                   >  1:1129622/1‑38 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCttg                                >  1:507482/1‑39 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                  >  1:5571374/1‑39 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                  >  1:1777286/1‑39 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                          >  1:3739970/1‑47 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                          >  1:1514323/1‑47 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                          >  1:5484388/1‑47 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                          >  1:3334255/1‑47 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                          >  1:1097490/1‑47 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAcc   <  1:429491/70‑1 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAc    >  1:1528072/1‑69 (MQ=255)
                                                                aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCt  >  1:5421536/1‑71 (MQ=255)
                                                                 cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGTTCACGCAGAATg            <  1:3467043/60‑1 (MQ=255)
                                                                 cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAGTg            <  1:2447274/60‑1 (MQ=255)
                                                                 cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg            <  1:4853194/60‑1 (MQ=255)
                                                                  |                                                                    
CAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCT  >  minE/1015729‑1015863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: