| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,795 | G→T | 17.4% | R92S (CGT→AGT) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,795 | 0 | G | T | 17.4% | 277.6 / 31.3 | 103 | R92S (CGT→AGT) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (62/23); new base T (8/10); total (70/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCT > minE/1015729‑1015863 | caaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg < 1:1928952/71‑1 (MQ=255) aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg > 1:3286898/1‑70 (MQ=255) taatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt > 1:2007616/1‑71 (MQ=255) atCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTg < 1:791435/71‑1 (MQ=255) atCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTg < 1:3132523/71‑1 (MQ=255) gCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca > 1:4192488/1‑71 (MQ=255) ggTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCcac > 1:1102281/1‑69 (MQ=255) tATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACata < 1:3301211/70‑1 (MQ=255) aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:3329695/1‑71 (MQ=255) aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:5109385/1‑71 (MQ=255) tCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCcacg < 1:5676077/66‑2 (MQ=255) tCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac < 1:4853166/69‑2 (MQ=255) tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGc < 1:1596908/57‑1 (MQ=255) tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca < 1:1046048/64‑1 (MQ=255) tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:542707/1‑70 (MQ=255) tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:4150612/1‑70 (MQ=255) cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTa < 1:3355582/70‑1 (MQ=255) cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTAt > 1:3885883/1‑71 (MQ=255) cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTAt > 1:1937606/1‑71 (MQ=255) tggcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTc > 1:3719848/4‑71 (MQ=255) gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg > 1:3165474/1‑52 (MQ=255) gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa > 1:2935923/1‑54 (MQ=255) gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa > 1:4178052/1‑54 (MQ=255) gACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa > 1:4388660/1‑54 (MQ=255) gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcgt > 1:198594/2‑69 (MQ=255) gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcg < 1:3760770/69‑2 (MQ=255) aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATc < 1:1445599/69‑1 (MQ=255) aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt > 1:1035501/1‑71 (MQ=255) cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt > 1:5096043/1‑71 (MQ=255) cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt < 1:4048499/71‑1 (MQ=255) gtcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTc < 1:184778/68‑1 (MQ=255) tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg < 1:800685/69‑1 (MQ=255) tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg < 1:4983230/69‑1 (MQ=255) ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc < 1:1965448/70‑1 (MQ=255) ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt > 1:3020013/1‑71 (MQ=255) cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg > 1:389951/1‑71 (MQ=255) cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg > 1:1444056/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:4096225/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:4326027/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:978119/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:321512/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:334766/1‑70 (MQ=255) aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa < 1:2324461/36‑1 (MQ=255) aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa < 1:2351181/36‑1 (MQ=255) aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCt < 1:2604459/71‑1 (MQ=255) aCGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt > 1:3612019/1‑65 (MQ=255) aCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa < 1:2073473/35‑1 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt > 1:1969188/1‑35 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt > 1:3904525/1‑35 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc > 1:4245253/1‑71 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc > 1:2402841/1‑71 (MQ=255) gCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGtttt < 1:4648872/59‑1 (MQ=255) ccTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg > 1:3510957/1‑68 (MQ=255) tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc < 1:1010751/57‑1 (MQ=255) tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc < 1:1200733/57‑1 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCcaca > 1:1137746/1‑42 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCcaca > 1:1340184/1‑42 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt > 1:2318724/1‑70 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGt > 1:5195157/1‑70 (MQ=255) tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:3601394/1‑57 (MQ=255) tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:3277650/1‑57 (MQ=255) tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:2722800/1‑57 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:5304385/1‑69 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:5350317/1‑69 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:53878/1‑69 (MQ=255) gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac > 1:3582023/1‑38 (MQ=12) gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac > 1:134986/1‑38 (MQ=12) gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACgtac > 1:3307507/1‑38 (MQ=12) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:3181149/71‑1 (MQ=255) gATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt < 1:3794902/45‑1 (MQ=255) atttCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAAt < 1:5408500/68‑1 (MQ=255) aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtttt > 1:3358815/1‑46 (MQ=255) aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt < 1:1164286/43‑1 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg > 1:4340196/1‑58 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg < 1:5305721/71‑1 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGc > 1:3968666/1‑64 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt > 1:1802020/1‑71 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt > 1:780844/1‑71 (MQ=255) ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:4184880/1‑48 (MQ=255) ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:3472034/1‑48 (MQ=255) ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTc < 1:1534111/38‑1 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg > 1:806016/1‑37 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg > 1:3437760/1‑37 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg > 1:3471870/1‑37 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:3403599/1‑38 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:1129622/1‑38 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCttg > 1:507482/1‑39 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt > 1:5571374/1‑39 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt > 1:1777286/1‑39 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:3739970/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:1514323/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:5484388/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:3334255/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:1097490/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAcc < 1:429491/70‑1 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAc > 1:1528072/1‑69 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCt > 1:5421536/1‑71 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGTTCACGCAGAATg < 1:3467043/60‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAGTg < 1:2447274/60‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg < 1:4853194/60‑1 (MQ=255) | CAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCT > minE/1015729‑1015863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |