| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,817 | T→C | 15.5% | K84K (AAA→AAG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,817 | 0 | T | C | 15.5% | 204.9 / 27.2 | 90 | K84K (AAA→AAG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (50/26); new base C (4/10); total (54/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
ATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGC > minE/1015748‑1015886 | aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:5109385/1‑71 (MQ=255)aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:3329695/1‑71 (MQ=255) tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:542707/1‑70 (MQ=255) tCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATAtt > 1:4150612/1‑70 (MQ=255) cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTa < 1:3355582/70‑1 (MQ=255) cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTAt > 1:1937606/1‑71 (MQ=255) cTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTAt > 1:3885883/1‑71 (MQ=255) tggcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTc > 1:3719848/4‑71 (MQ=255) gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcgt > 1:198594/2‑69 (MQ=255) gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcg < 1:3760770/69‑2 (MQ=255) aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATc < 1:1445599/69‑1 (MQ=255) aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt > 1:4058642/1‑71 (MQ=255) aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt > 1:1035501/1‑71 (MQ=255) cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt > 1:5096043/1‑71 (MQ=255) cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt < 1:4048499/71‑1 (MQ=255) gtcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTc < 1:184778/68‑1 (MQ=255) tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg < 1:4983230/69‑1 (MQ=255) tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg < 1:800685/69‑1 (MQ=255) ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc < 1:1965448/70‑1 (MQ=255) ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt > 1:3020013/1‑71 (MQ=255) cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg > 1:389951/1‑71 (MQ=255) cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg > 1:1444056/1‑71 (MQ=255) aGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:2746248/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:4096225/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:321512/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:4326027/1‑71 (MQ=255) gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:334766/1‑70 (MQ=255) aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCt < 1:2604459/71‑1 (MQ=255) aCGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt > 1:3612019/1‑65 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc > 1:2402841/1‑71 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc > 1:4245253/1‑71 (MQ=255) gCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGtttt < 1:4648872/59‑1 (MQ=255) ccTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg > 1:3510957/1‑68 (MQ=255) tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc < 1:1200733/57‑1 (MQ=255) tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc < 1:1010751/57‑1 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt > 1:2318724/1‑70 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGt > 1:5195157/1‑70 (MQ=255) tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:2722800/1‑57 (MQ=255) tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:3601394/1‑57 (MQ=255) tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:3277650/1‑57 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:5350317/1‑69 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:53878/1‑69 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:5304385/1‑69 (MQ=255) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:3181149/71‑1 (MQ=255) gATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt < 1:3794902/45‑1 (MQ=255) atttCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAAt < 1:5408500/68‑1 (MQ=255) aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtttt > 1:3358815/1‑46 (MQ=255) aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt < 1:1164286/43‑1 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg < 1:5305721/71‑1 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGc > 1:3968666/1‑64 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt > 1:780844/1‑71 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt > 1:1802020/1‑71 (MQ=255) ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:3472034/1‑48 (MQ=255) ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc > 1:4184880/1‑48 (MQ=255) ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTc < 1:1534111/38‑1 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg > 1:806016/1‑37 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg > 1:3471870/1‑37 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg > 1:3437760/1‑37 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:3403599/1‑38 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc > 1:1129622/1‑38 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCttg > 1:507482/1‑39 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt > 1:1777286/1‑39 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt > 1:5571374/1‑39 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:3739970/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:1097490/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:3334255/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:5484388/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:1514323/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAcc < 1:429491/70‑1 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAc > 1:1528072/1‑69 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCt > 1:5421536/1‑71 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGTTCACGCAGAATg < 1:3467043/60‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAGTg < 1:2447274/60‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg < 1:4853194/60‑1 (MQ=255) aagAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgt < 1:4656579/67‑1 (MQ=255) aGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttg < 1:4675294/71‑1 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:4551879/1‑71 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:4422276/1‑71 (MQ=255) gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct < 1:5373429/71‑4 (MQ=255) gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct < 1:454642/71‑4 (MQ=255) gAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt < 1:1863648/71‑1 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGaa > 1:1826152/1‑52 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGttgttg < 1:1931121/71‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt < 1:1562051/53‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt < 1:4871374/53‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt < 1:5392302/68‑1 (MQ=255) gCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgc > 1:875784/1‑71 (MQ=255) cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg < 1:396762/71‑1 (MQ=255) aGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTgtgc < 1:5010150/71‑1 (MQ=255) cacaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgtt < 1:3193465/56‑1 (MQ=255) acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTgg < 1:4727920/60‑1 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGcc < 1:4517514/46‑1 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt < 1:5620737/55‑1 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt > 1:1200501/1‑59 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt > 1:1850817/1‑59 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTg > 1:2582099/1‑60 (MQ=255) tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag > 1:4616315/1‑71 (MQ=255) tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag > 1:2084705/1‑71 (MQ=255) tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag > 1:2288621/1‑71 (MQ=255) tttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcagc < 1:1409482/70‑1 (MQ=255) | ATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGC > minE/1015748‑1015886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |