Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 218,097 G→A 12.0% L28L (TTG→TTA yafF → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE218,0970GA12.0% 770.6 / 69.2 292L28L (TTG→TTAyafFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (131/125);  new base A (15/20);  total (146/146)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 298 total aligned reads displayed.

CTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAC  >  minE/218028‑218163
                                                                     |                                                                  
ctgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa                                                                   >  1:5377879/4‑71 (MQ=255)
   cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATAAGGCACATTGCTATTAATATTTTGACg                                                                 >  1:4748679/1‑70 (MQ=255)
   cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg                                                                 >  1:5263405/1‑70 (MQ=255)
    aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                               <  1:5492118/71‑1 (MQ=255)
      aaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt                                                              >  1:4466256/1‑70 (MQ=255)
       aTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATga                                                            <  1:3439115/71‑1 (MQ=255)
       aTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATga                                                            >  1:264528/1‑71 (MQ=255)
        tAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                               >  1:379098/1‑67 (MQ=255)
        tAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATga                                                            >  1:4359183/1‑70 (MQ=255)
          agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata                                                          <  1:2407171/70‑3 (MQ=255)
          agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgat                                                           >  1:606327/1‑68 (MQ=255)
              gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                                       <  1:3475987/69‑1 (MQ=255)
               agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt                                                    <  1:4941815/71‑1 (MQ=255)
                ggAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt                                                              <  1:553203/60‑1 (MQ=255)
                 gAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                  <  1:1141181/71‑1 (MQ=255)
                 gAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc                                                  <  1:930754/71‑1 (MQ=255)
                  aaaTGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                  >  1:1071531/1‑70 (MQ=255)
                   aatgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                  >  1:682518/4‑69 (MQ=255)
                   aatgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCaa                                                <  1:2613643/68‑1 (MQ=255)
                     tgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAgg                                              <  1:2716167/70‑1 (MQ=255)
                      gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                              >  1:563259/1‑70 (MQ=255)
                       cagcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                              >  1:2306825/1‑69 (MQ=255)
                       cagcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCa                                            <  1:1886386/71‑1 (MQ=255)
                        agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc                                                                  >  1:3677220/1‑48 (MQ=255)
                        agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc                                                                  >  1:2223306/1‑48 (MQ=255)
                            gAATTATTTTAAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGtt                                       <  1:2445970/71‑1 (MQ=255)
                              attattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                  <  1:413250/58‑1 (MQ=255)
                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa                                                                <  1:1650379/43‑1 (MQ=255)
                                tattTTCAGGGATACGGCACATTCTATTAATATTTTGAc                                                                  >  1:951582/1‑39 (MQ=37)
                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGa                                                                   >  1:4638921/1‑38 (MQ=255)
                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt                                                              >  1:1960637/1‑43 (MQ=255)
                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                                  >  1:4511087/1‑71 (MQ=255)
                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                                  >  1:826375/1‑71 (MQ=255)
                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGAc                                  >  1:4572908/1‑71 (MQ=255)
                                  ttttCAGGGATACGGCACATTTCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                              <  1:1593237/58‑1 (MQ=255)
                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATg                                                             >  1:882100/1‑43 (MQ=255)
                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATg                                                             >  1:3516068/1‑43 (MQ=255)
                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                                  >  1:3702722/1‑70 (MQ=255)
                                    ttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTa                                                     >  1:3741900/1‑49 (MQ=255)
                                    ttCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                <  1:3501800/70‑1 (MQ=255)
                                       aGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                >  1:4615577/1‑67 (MQ=255)
                                        gggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAg                             <  1:5056319/69‑1 (MQ=255)
                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                                  <  1:1076976/47‑1 (MQ=255)
                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGAt                           >  1:5070107/1‑70 (MQ=255)
                                          gATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGc                         <  1:5436065/71‑1 (MQ=255)
                                           aTACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGc                         >  1:1540598/1‑70 (MQ=255)
                                           aTACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGc                         >  1:4872287/1‑70 (MQ=255)
                                            tACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCa                                                 <  1:4424168/45‑1 (MQ=255)
                                            tACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGt                                        <  1:4252661/54‑1 (MQ=255)
                                             aCGGCACATTGCTATTAATATTTTGACGAATGATaa                                                         >  1:2332929/1‑36 (MQ=255)
                                             aCGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa                       >  1:76636/1‑70 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCa                                                 >  1:5556179/1‑43 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGc                                             <  1:4791961/47‑1 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaaa                     <  1:2475371/71‑1 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaaa                     <  1:1776470/71‑1 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                      >  1:1233115/1‑70 (MQ=255)
                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                      >  1:4851396/1‑70 (MQ=255)
                                               ggCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                      >  1:5130413/1‑69 (MQ=255)
                                                 cacaTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagc                  <  1:4738183/71‑1 (MQ=255)
                                                   caTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGc                         <  1:1933144/62‑1 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc               <  1:517674/71‑1 (MQ=255)
                                                    aTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGt                                <  1:4618368/54‑1 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGATAAGGTATTCa                                                 >  1:1477973/1‑36 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc               >  1:367597/1‑70 (MQ=255)
                                                     ttGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc               >  1:1312504/1‑70 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGATAAGGTATTCaa                                                >  1:4905650/1‑35 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGATAAGGTATTCaa                                                >  1:3295413/1‑35 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt            >  1:1272297/1‑71 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt            >  1:316621/1‑71 (MQ=255)
                                                       gCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt            >  1:3602216/1‑71 (MQ=255)
                                                        cTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAg                                           >  1:3391110/1‑39 (MQ=255)
                                                        cTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt            <  1:3260884/70‑1 (MQ=255)
                                                        cTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATg                          <  1:5115329/56‑1 (MQ=255)
                                                         tATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCAt            >  1:2032868/1‑69 (MQ=255)
                                                            tAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGa                                   >  1:1689517/1‑43 (MQ=255)
                                                            tAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                <  1:2951614/46‑1 (MQ=255)
                                                            tAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                                <  1:1510548/46‑1 (MQ=255)
                                                            tAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag                   >  1:358126/1‑59 (MQ=255)
                                                            tAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTaa                              >  1:2990421/1‑48 (MQ=255)
                                                             aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTaa                              >  1:3095049/1‑47 (MQ=255)
                                                             aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa         >  1:3074008/1‑68 (MQ=255)
                                                             aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACa       <  1:5304034/70‑1 (MQ=255)
                                                             aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAg                                    <  1:4069794/41‑1 (MQ=255)
                                                             aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAg                                    <  1:4197259/41‑1 (MQ=255)
                                                             aaTATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGa         >  1:3035325/1‑68 (MQ=255)
                                                              atatTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGa                                   >  1:2096519/1‑41 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTaa                              <  1:3997142/45‑1 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAg                             >  1:2390569/1‑46 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa     >  1:4715980/1‑70 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa     <  1:1412990/70‑1 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa     <  1:4680091/70‑1 (MQ=255)
                                                               tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa     >  1:2251442/1‑70 (MQ=255)
                                                               tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc               <  1:2861242/60‑1 (MQ=255)
                                                                 ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa                      <  1:2682839/51‑1 (MQ=255)
                                                                 ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc               <  1:4563836/58‑1 (MQ=255)
                                                                 ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAg                                    >  1:1943855/1‑37 (MQ=255)
                                                                 ttttAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGaa    <  1:2770489/69‑1 (MQ=255)
                                                                     gACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                <  1:3841797/53‑1 (MQ=255)
                                                                     gACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcag                <  1:5605300/53‑1 (MQ=255)
                                                                     gACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCa             >  1:2147033/1‑56 (MQ=255)
                                                                     |                                                                  
CTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAC  >  minE/218028‑218163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: