| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,036,915 | C→G | 11.3% | A28G (GCC→GGC) | rsxD → | predicted inner membrane oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,036,915 | 0 | C | G | 11.3% | 285.1 / 22.2 | 106 | A28G (GCC→GGC) | rsxD | predicted inner membrane oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (51/43); new base G (5/7); total (56/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
ATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCG > minE/1036845‑1036985 | aTAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGc > 1:3382317/1‑71 (MQ=255) gctCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGcc < 1:1489575/70‑1 (MQ=255) ccTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAAt < 1:1772019/71‑1 (MQ=255) cTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGcc > 1:5116898/1‑59 (MQ=255) cTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGaa > 1:2678016/1‑69 (MQ=255) cTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGaa > 1:2838546/1‑69 (MQ=255) ataCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCg > 1:3631476/1‑57 (MQ=255) ataCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCg > 1:385278/1‑57 (MQ=255) taCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGt > 1:3585326/1‑57 (MQ=255) taCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCg < 1:1363671/68‑1 (MQ=255) taCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAg < 1:3267009/71‑1 (MQ=255) aCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgc > 1:477417/1‑71 (MQ=255) cccATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgc < 1:4615925/70‑1 (MQ=255) cATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcgc > 1:5234443/1‑70 (MQ=255) cATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcgc > 1:870967/1‑70 (MQ=255) cATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCa < 1:2852301/71‑1 (MQ=255) aTAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa < 1:1019901/71‑1 (MQ=255) aTAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa < 1:3368504/71‑1 (MQ=255) aTAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa < 1:2037839/71‑1 (MQ=255) tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTTCCAGGAATCGc < 1:4354260/63‑1 (MQ=255) tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAAt > 1:2359107/1‑60 (MQ=255) tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGc < 1:4024904/63‑1 (MQ=255) tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAAc > 1:1540604/1‑71 (MQ=255) aaacAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACt > 1:61651/4‑71 (MQ=255) aaCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACt > 1:1337783/1‑71 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGc > 1:616715/1‑51 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGc > 1:3572395/1‑51 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCTAGGAATCGCAGCGCAACt > 1:4335697/1‑70 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg > 1:2047406/1‑55 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAg > 1:701602/1‑54 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGa > 1:5127305/1‑56 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg > 1:1428034/1‑71 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg < 1:2262150/71‑1 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg > 1:4161347/1‑71 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg < 1:3781144/71‑1 (MQ=255) aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg > 1:189542/1‑71 (MQ=255) ccAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATc < 1:2675933/58‑1 (MQ=255) ccAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATc < 1:2580222/58‑1 (MQ=255) ccAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCa > 1:5667701/1‑61 (MQ=255) gcCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg < 1:5571735/49‑1 (MQ=255) gcCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg < 1:3956729/49‑1 (MQ=255) gcCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGttt > 1:5472455/1‑71 (MQ=255) ccAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGaa < 1:4915510/50‑1 (MQ=255) ccAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtt < 1:1912598/69‑1 (MQ=255) cAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGc > 1:4997507/1‑43 (MQ=255) aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt < 1:3002321/71‑1 (MQ=255) aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt < 1:3961225/71‑1 (MQ=255) aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt < 1:2370063/71‑1 (MQ=255) aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt < 1:2086494/71‑1 (MQ=255) gACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttg < 1:1770833/71‑1 (MQ=255) gACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttg < 1:4893702/71‑1 (MQ=255) cATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt < 1:5177379/71‑1 (MQ=255) cATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt < 1:3225673/71‑1 (MQ=255) gcgcATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGttttt > 1:1394471/1‑63 (MQ=255) gcgcATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGttttt > 1:4407964/1‑63 (MQ=255) cgcATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt < 1:2497912/66‑1 (MQ=255) aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa > 1:380299/1‑45 (MQ=255) aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa > 1:5158545/1‑45 (MQ=255) aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa > 1:449340/1‑45 (MQ=255) aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttg > 1:906235/1‑58 (MQ=255) aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTActc > 1:4823916/1‑70 (MQ=255) aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTActc < 1:2946173/70‑1 (MQ=255) gCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggttg < 1:466619/60‑1 (MQ=255) cTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt > 1:904874/1‑52 (MQ=255) cTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCg < 1:3341867/70‑1 (MQ=255) ttGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGtt < 1:3644329/69‑1 (MQ=255) tGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt > 1:3013343/1‑48 (MQ=255) tGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt > 1:130791/1‑48 (MQ=255) tGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt > 1:2024024/1‑48 (MQ=255) gtgtTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGtt > 1:4007974/1‑66 (MQ=255) gtTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtt < 1:1056520/42‑1 (MQ=255) attgCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc < 1:4407867‑M1/44‑28 (MQ=255) attgCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc < 1:83254‑M1/44‑28 (MQ=255) attgCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc < 1:1634870‑M1/44‑28 (MQ=255) ttGCTCGCAGGCGTGCCttttgccatacacattgaaagacga < 1:1091202‑M1/42‑26 (MQ=255) ttGCTCGCAGGCGTGCCgtttgccatacacattgaaagacga < 1:802490‑M1/42‑26 (MQ=255) ttGCTCGCAGGCGTGCCgtttgccatacacattgaaaga > 1:3475854‑M1/1‑17 (MQ=255) ttGCTCGCAGGCGTGCCgtttgccatacacattgaaaga > 1:82173‑M1/1‑17 (MQ=255) ttGCTCGCAGGCGTGCCgtttgccatacacattgaaaga > 1:1614225‑M1/1‑17 (MQ=255) ttGCTCGCAGGCGTGCCgtttgccatacacattgaaag > 1:2396428‑M1/1‑17 (MQ=255) tGCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc > 1:4957110‑M1/1‑16 (MQ=255) tGCTCGCAGGCGTGCCgtttgccatacacattgaaa < 1:610549‑M1/36‑21 (MQ=255) tGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTg > 1:410635/1‑71 (MQ=255) tGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTg > 1:1638132/1‑71 (MQ=255) gCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCg > 1:934218/1‑59 (MQ=255) tCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTg < 1:1829806/68‑1 (MQ=255) cGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggttg > 1:4851937/1‑45 (MQ=255) cGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTgg > 1:845769/1‑71 (MQ=255) cGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTg > 1:1208721/1‑70 (MQ=255) gCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTg < 1:5227757/69‑1 (MQ=255) gCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGc < 1:2490813/71‑1 (MQ=255) cggCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGa > 1:2412435/3‑60 (MQ=255) cAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTg > 1:2338333/1‑68 (MQ=255) cAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCa < 1:1120512/71‑1 (MQ=255) cAGCCGTGCCAGGAATCGCAGCGCAACTGTGGGTTTTTGGTTGGGGTACTCTCGTTCAGAACCTGTTg > 1:1605485/1‑68 (MQ=255) aGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTc > 1:1452164/1‑56 (MQ=255) aGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCAt < 1:5479527/71‑1 (MQ=255) gCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATc < 1:4295419/71‑1 (MQ=255) ccGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATc < 1:753031/70‑1 (MQ=255) ccGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCg < 1:4871551/71‑1 (MQ=255) | ATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCG > minE/1036845‑1036985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |