Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,201,879 T→C 13.6% G344G (GGT→GGC)  sdaA → L‑serine deaminase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,201,8790TC13.6% 167.4 / 14.8 66G344G (GGT→GGC) sdaAL‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (4/5);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGG  >  minE/1201812‑1201940
                                                                   |                                                             
ttGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGtt                                                            >  1:786844/1‑71 (MQ=255)
ttGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGtt                                                            >  1:3776762/1‑71 (MQ=255)
 tGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGtt                                                            >  1:3453460/1‑70 (MQ=255)
   gTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGcc                                                         <  1:5654169/71‑1 (MQ=255)
    tGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCt                                                        <  1:2979584/71‑1 (MQ=255)
      cATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGt                                                      >  1:2230101/1‑71 (MQ=255)
      cATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGt                                                      >  1:3849571/1‑71 (MQ=255)
      cATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGt                                                      >  1:419170/1‑71 (MQ=255)
          gTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                              >  1:2109799/1‑59 (MQ=255)
          gTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                                  >  1:4111029/1‑71 (MQ=255)
           tataAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTc                                                    <  1:2274963/68‑1 (MQ=9)
           tataAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAAt                                                 >  1:2170275/1‑71 (MQ=255)
            ataAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGAGAAGTGGGTGTTGCCTGTTc                                                    <  1:1090400/67‑1 (MQ=255)
            aaaaaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTc                                                    <  1:1201554/65‑1 (MQ=255)
              aaaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGgc                                              >  1:2639675/1‑71 (MQ=255)
                aaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGgctg                                            >  1:2878333/1‑71 (MQ=255)
                aaTCAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGgctg                                            <  1:4324716/71‑1 (MQ=255)
                   gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCaa                                                  >  1:3672582/1‑62 (MQ=9)
                   gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCaa                                                  >  1:885873/1‑62 (MQ=9)
                          tctATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCt                                  >  1:1281896/1‑71 (MQ=255)
                          tcgaTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCt                                  <  1:3856265/68‑1 (MQ=2)
                           ctATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATg                                                <  1:2330361/56‑1 (MQ=255)
                           ctATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATg                                                <  1:5575919/56‑1 (MQ=255)
                            tATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCtt                                 >  1:1033113/1‑70 (MQ=255)
                             aTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGgcgtgg                                                           <  1:3633851/43‑3 (MQ=16)
                             aTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGc                                                          >  1:3698550/1‑44 (MQ=9)
                             aTTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTg                                >  1:423164/1‑70 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                              >  1:2060467/1‑39 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                              >  1:491725/1‑39 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                              >  1:3539557/1‑39 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTc                                                    <  1:2981976/49‑1 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTg                                <  1:1414700/69‑1 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTg                                <  1:5639978/69‑1 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGc                               <  1:589542/70‑1 (MQ=255)
                              tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGc                               <  1:1377739/70‑1 (MQ=255)
                                tctggTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAg                             <  1:2404421/67‑1 (MQ=2)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGc                                                          <  1:4917877/41‑1 (MQ=255)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGc                                                          <  1:5364911/41‑1 (MQ=255)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg                             >  1:3259618/1‑70 (MQ=255)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg                             >  1:871001/1‑70 (MQ=255)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg                             <  1:5555480/70‑1 (MQ=255)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg                             <  1:2082192/70‑1 (MQ=255)
                                tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg                             >  1:1969824/1‑70 (MQ=255)
                                  tggTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTc                                                    <  1:2748762/44‑1 (MQ=9)
                                    gTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAActg                        >  1:4693927/1‑71 (MQ=255)
                                     tGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAAt                                                 <  1:2913148/45‑1 (MQ=255)
                                       cGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg                             <  1:4713454/63‑1 (MQ=255)
                                         gAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGaa                           >  1:1592768/1‑63 (MQ=255)
                                          aaGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGaa                           >  1:5674933/1‑62 (MQ=255)
                                          aaGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGc                  <  1:3233971/71‑1 (MQ=255)
                                           aGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCg                 <  1:5418114/71‑1 (MQ=255)
                                            gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                                  <  1:4013511/37‑1 (MQ=255)
                                            gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                                  <  1:2824695/37‑1 (MQ=255)
                                            gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                                  <  1:4128865/37‑1 (MQ=255)
                                            gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                                  <  1:3918597/37‑1 (MQ=255)
                                            gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCgg                <  1:1424339/71‑1 (MQ=255)
                                              tggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCTGGTCTTGCAGAACTGCTGGGCgg                >  1:4521925/1‑69 (MQ=255)
                                                   gCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAc                          >  1:1833288/1‑54 (MQ=255)
                                                    ccAGGGCGAAGGGGGTGTTGCCTGTTCATTGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCgg        <  1:1182769/71‑1 (MQ=255)
                                                      aGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGaa      <  1:3166870/71‑1 (MQ=255)
                                                        ggCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGaa      >  1:3446130/1‑69 (MQ=255)
                                                        ggCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACa    <  1:3030667/71‑1 (MQ=255)
                                                         gCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCgg                                      >  1:1693374/1‑36 (MQ=255)
                                                          cGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCgg                                      >  1:3911905/1‑35 (MQ=255)
                                                          cGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAgg  >  1:3035593/1‑71 (MQ=255)
                                                              gTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCgg        <  1:1202781/61‑1 (MQ=255)
                                                               ggggTGTTGCCTGTTCTATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACa    <  1:2221695/63‑1 (MQ=255)
                                                                gggTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAct                         >  1:4878017/1‑42 (MQ=255)
                                                                gggTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAct                         >  1:5037562/1‑42 (MQ=255)
                                                                gggTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAct                         >  1:5320347/1‑42 (MQ=255)
                                                                gggTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAct                         >  1:2888979/1‑42 (MQ=255)
                                                                   |                                                             
TTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGG  >  minE/1201812‑1201940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑