| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,240 | C→T | 25.4% | G38S (GGT→AGT) ‡ | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,240 | 0 | C | T | 25.4% | 210.9 / 66.3 | 114 | G38S (GGT→AGT) ‡ | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base C (37/48); new base T (8/21); total (45/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTT > minE/1211173‑1211308 | ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:773513/71‑1 (MQ=255)ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:575558/71‑1 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:626907/1‑69 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa < 1:2718993/69‑1 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:2660368/1‑69 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc < 1:4264330/69‑1 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:1299178/70‑1 (MQ=255) cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:3148808/71‑1 (MQ=255) cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:1933932/71‑1 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:63875/1‑62 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:3579978/1‑70 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATAACCCTGGa > 1:321761/1‑62 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAccc < 1:2603352/57‑1 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:854272/1‑69 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga > 1:1729316/1‑68 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg > 1:57372/1‑71 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:3133154/68‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:5304883/69‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:1780813/71‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa > 1:5393203/1‑71 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:5534542/71‑1 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc > 1:1514981/1‑71 (MQ=255) aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:5208823/70‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:1315390/71‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:394843/71‑1 (MQ=255) tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa < 1:2607437/70‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:2481993/1‑47 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:4910506/62‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:202511/1‑70 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:2992507/1‑70 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:5322828/1‑43 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:3794890/1‑43 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTgg > 1:532067/1‑43 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:2265028/1‑44 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:4738548/1‑71 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:4306100/51‑1 (MQ=255) ctttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAgt > 1:3540719/3‑54 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:2389265/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:148853/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:5516915/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:1834766/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:5339600/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg < 1:1692410/71‑1 (MQ=255) tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAgaga < 1:3311892/49‑1 (MQ=255) gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:2607791/1‑36 (MQ=255) cgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgc < 1:5022279/68‑1 (MQ=255) tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg > 1:4519688/1‑71 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:1254573/1‑71 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGAt < 1:5508843/38‑1 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:468109/1‑70 (MQ=255) gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa > 1:5551196/1‑71 (MQ=255) gACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGa > 1:2001823/1‑36 (MQ=16) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:3684160/47‑1 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:1258506/47‑1 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:138043/47‑1 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:4446477/47‑1 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:3933927/1‑71 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:2276753/1‑71 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:1788695/58‑1 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:5328213/58‑1 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:3821948/58‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga > 1:1257439/1‑39 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa > 1:574708/1‑45 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:3489174/69‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:1528557/69‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc < 1:1600824/70‑1 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:2632015/71‑1 (MQ=255) ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:3270136/35‑1 (MQ=255) aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg > 1:2641007/2‑71 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact < 1:2794389/71‑3 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:5312850/1‑70 (MQ=255) agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac < 1:2637403/43‑1 (MQ=16) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5075619/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2661813/1‑71 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2186337/1‑71 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:2078635/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5394219/71‑1 (MQ=255) gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt < 1:1547175/71‑1 (MQ=16) tttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGccg < 1:5545042/55‑2 (MQ=16) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:1533115/70‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:2205268/70‑1 (MQ=255) tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg > 1:1975074/1‑71 (MQ=255) gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc > 1:661413/1‑42 (MQ=255) aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc < 1:663283/68‑1 (MQ=12) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc > 1:431655/1‑70 (MQ=255) ccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt > 1:1058512/1‑41 (MQ=19) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2245700/71‑1 (MQ=255) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:3652710/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:2481815/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:951577/70‑1 (MQ=9) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:5092732/70‑1 (MQ=9) cATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGa > 1:2581149/1‑56 (MQ=12) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:597120/1‑71 (MQ=255) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3805245/1‑71 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa < 1:3383326/67‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:1814370/35‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:2290882/35‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:2382160/35‑1 (MQ=255) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGATGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:4280542/1‑70 (MQ=255) cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:4050024/70‑1 (MQ=255) | CCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTT > minE/1211173‑1211308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |