| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 222,967 | A→G | 11.0% | intergenic (+22/‑158) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 222,967 | 0 | A | G | 11.0% | 206.3 / 14.2 | 82 | intergenic (+22/‑158) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base A (22/51); new base G (5/4); total (27/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
GTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTA > minE/222898‑223035 | gTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGAc < 1:1581574/71‑1 (MQ=255) tGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACg < 1:1548591/71‑1 (MQ=255) aTCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:3919900/71‑1 (MQ=255) aTCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:1213214/71‑1 (MQ=255) tCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:5485903/70‑1 (MQ=255) tCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:1113132/70‑1 (MQ=255) tCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt > 1:4519338/1‑70 (MQ=255) ccATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:4063601/69‑1 (MQ=255) ccATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:4873823/69‑1 (MQ=255) ccATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:2842043/69‑1 (MQ=255) cATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAc < 1:2910442/71‑1 (MQ=255) tatCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGttt < 1:4165898/67‑1 (MQ=255) tatCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGttt < 1:4884608/67‑1 (MQ=255) tatCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTActc > 1:2535177/1‑69 (MQ=255) tCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTa > 1:4807687/1‑66 (MQ=255) tCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcaga > 1:1883630/1‑67 (MQ=255) cTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCa < 1:1607679/71‑1 (MQ=255) cTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGCATAAGACGTTTACGCCGCa < 1:767501/71‑1 (MQ=255) cAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGc < 1:2904642/71‑1 (MQ=255) cAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGc < 1:1044458/71‑1 (MQ=255) cAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGc < 1:1881737/71‑1 (MQ=255) cAGTTGATTGAAAAAGAGATGGGTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGc < 1:3162118/71‑1 (MQ=255) gTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCa < 1:554089/63‑1 (MQ=255) gTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAt < 1:3064617/71‑1 (MQ=255) gTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAt < 1:2530753/71‑1 (MQ=255) ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCTGCAtt < 1:41700/71‑1 (MQ=255) ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt < 1:570566/71‑1 (MQ=255) ttgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:2418716/49‑1 (MQ=255) ttgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:67338/49‑1 (MQ=255) ttgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:3322679/49‑1 (MQ=255) ttgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:4414501/49‑1 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:1090087/71‑1 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:3333644/71‑1 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct > 1:155804/1‑71 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:44626/44‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:846465/44‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:3634691/44‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:3771522/44‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg < 1:764669/71‑1 (MQ=255) aaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:2893171/43‑1 (MQ=255) aaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:1809685/43‑1 (MQ=255) aaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:1649470/43‑1 (MQ=255) aaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGtt < 1:1316004/43‑1 (MQ=255) aaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTa > 1:1095810/1‑45 (MQ=255) aaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTa > 1:1610853/1‑45 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 1:1296964/1‑71 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 1:3852053/1‑71 (MQ=255) agaTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg > 1:1077818/1‑64 (MQ=255) tGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctg < 1:1196182/71‑1 (MQ=255) gTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTAcgccg > 1:659022/1‑42 (MQ=255) gTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAttt > 1:3970016/1‑54 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg < 1:3505789/70‑1 (MQ=255) agtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCt < 1:1655151/71‑1 (MQ=255) cTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc > 1:434380/1‑71 (MQ=255) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGcagatgcct < 1:2467046/50‑1 (MQ=9) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:3991590/70‑1 (MQ=9) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:4325091/70‑1 (MQ=9) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgc > 1:1101933/1‑41 (MQ=255) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa > 1:4039942/1‑71 (MQ=255) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa < 1:3968490/71‑1 (MQ=255) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:3118110/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4582333/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4683535/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:2766014/1‑70 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGAATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:834604/70‑1 (MQ=2) gCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTa > 1:4619478/1‑70 (MQ=11) cGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt < 1:2226868/64‑1 (MQ=14) ggATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGcagatgcctgatgc < 1:422928/50‑1 (MQ=11) ggATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGcagatgcctgatgc < 1:1756742/50‑1 (MQ=11) ggATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:1063915/1‑68 (MQ=14) ggACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTATc < 1:3573976/67‑1 (MQ=9) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:4350560/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:5554197/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:824945/1‑69 (MQ=11) gATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:1701772/44‑1 (MQ=255) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctga > 1:3616525/1‑45 (MQ=255) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctga > 1:2609340/1‑45 (MQ=255) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctga > 1:1805980/1‑45 (MQ=255) tAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGcc < 1:3814930/71‑1 (MQ=11) tAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 1:4290447/1‑37 (MQ=255) aGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:3666333/1‑71 (MQ=255) aGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:989136/1‑71 (MQ=255) | GTGATCCATATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTA > minE/222898‑223035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |