| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,280 | A→G | 32.0% | D24D (GAT→GAC) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,280 | 0 | A | G | 32.0% | 209.4 / 119.6 | 147 | D24D (GAT→GAC) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base A (47/53); new base G (25/22); total (72/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 149 total aligned reads displayed.
GACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCA > minE/1211210‑1211350 | gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa > 1:5551196/1‑71 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:2276753/1‑71 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:3489174/69‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:1528557/69‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc < 1:1600824/70‑1 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:2632015/71‑1 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact < 1:2794389/71‑3 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:5312850/1‑70 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:1759384/71‑1 (MQ=255) agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa < 1:5371059/71‑1 (MQ=255) agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa < 1:4061192/71‑1 (MQ=16) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:2078635/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2661813/1‑71 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2186337/1‑71 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5075619/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5394219/71‑1 (MQ=255) gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt < 1:1547175/71‑1 (MQ=16) ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:4816761/71‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:2655222/70‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:1533115/70‑1 (MQ=255) aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc < 1:663283/68‑1 (MQ=12) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc > 1:431655/1‑70 (MQ=255) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2066571/71‑1 (MQ=255) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2245700/71‑1 (MQ=255) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:3652710/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:951577/70‑1 (MQ=9) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:5092732/70‑1 (MQ=9) cATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGa > 1:2581149/1‑56 (MQ=12) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:597120/1‑71 (MQ=255) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3805245/1‑71 (MQ=255) ctcTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt < 1:2084618/69‑1 (MQ=9) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3886370/70‑1 (MQ=255) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGATGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:4280542/1‑70 (MQ=255) cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:4050024/70‑1 (MQ=255) cTGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:2917133/1‑68 (MQ=255) tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaaac > 1:5419329/1‑56 (MQ=18) tGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa > 1:290164/1‑71 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGa > 1:4172713/1‑69 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt > 1:2843540/1‑71 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:3655965/63‑1 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt < 1:3159219/68‑1 (MQ=12) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTa < 1:1732270/44‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:303111/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:5539536/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:1613290/71‑1 (MQ=255) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:2598203/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:3861295/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCAGGAGTAATGAAACCGAATCCtttg < 1:1869002/60‑2 (MQ=12) gagaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:120909/1‑44 (MQ=255) gagaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:3001490/1‑44 (MQ=255) aaGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3736081/55‑1 (MQ=255) aGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCAc < 1:4487975/71‑1 (MQ=255) tgtACGAACACATCTTTGCTGCCATCAGCCGGAGTa < 1:5235387/36‑1 (MQ=255) tgtAAGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAccg < 1:1828006/45‑2 (MQ=2) tgtAAGAACACGTCATTGCTGCCGTCTTCCGGAGTAATGATACCGAATCCtt < 1:3246410/52‑1 (MQ=255) gtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg > 1:1348652/1‑38 (MQ=18) gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:1027758/38‑1 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaaa < 1:4490316/40‑2 (MQ=17) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:4421765/1‑36 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:3011408/1‑36 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa > 1:4843779/1‑52 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt > 1:2812273/1‑59 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:2181620/70‑1 (MQ=255) tACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAtttta > 1:4551257/1‑69 (MQ=255) gaCGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAAc > 1:893405/2‑47 (MQ=12) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3856104/1‑46 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:1208086/1‑46 (MQ=255) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa > 1:2849694/1‑36 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa > 1:3804661/1‑36 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2622983/1‑62 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg > 1:192844/1‑70 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc < 1:3081630/70‑1 (MQ=12) gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt > 1:4944556/1‑71 (MQ=255) aaCACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcgtt < 1:4154567/71‑4 (MQ=12) aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga > 1:1397473/1‑38 (MQ=9) aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga > 1:5206818/1‑38 (MQ=9) aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga > 1:1525315/1‑38 (MQ=9) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa < 1:3624785/71‑1 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa < 1:3971008/71‑1 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:476376/61‑1 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:3855277/61‑1 (MQ=255) cgtCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCt > 1:3944241/3‑71 (MQ=12) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:1152790/71‑1 (MQ=255) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:4781149/71‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg > 1:4588488/1‑43 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:5158342/60‑1 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:4205672/1‑36 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa > 1:5689306/1‑60 (MQ=255) tttGGTGCCATCAGCCGGAGTAATGAAGCCAAAACCt > 1:5021466/1‑37 (MQ=255) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg < 1:1025835/41‑3 (MQ=12) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg < 1:4885998/40‑2 (MQ=12) tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg > 1:5654221/1‑63 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:3454643/68‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:2164328/68‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:3678572/68‑1 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:5137044/1‑71 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:3595011/1‑71 (MQ=255) ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtt < 1:5041135/71‑1 (MQ=255) ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCt < 1:3743808/70‑1 (MQ=255) ccATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc > 1:1797797/1‑71 (MQ=255) | GACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCA > minE/1211210‑1211350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |