| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,286 | C→A | 7.9% | P22P (CCG→CCT) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,286 | 0 | C | A | 7.9% | 422.1 / 14.4 | 150 | P22P (CCG→CCT) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base C (66/72); new base A (8/4); total (74/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
Only 100 of 161 total aligned reads displayed.
AGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTG > minE/1211217‑1211354 | aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg > 1:2641007/2‑71 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact < 1:2794389/71‑3 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:5312850/1‑70 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:1759384/71‑1 (MQ=255) agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa < 1:5371059/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5394219/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2186337/1‑71 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5075619/71‑1 (MQ=255) gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt < 1:1547175/71‑1 (MQ=16) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:2655222/70‑1 (MQ=255) aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc < 1:663283/68‑1 (MQ=12) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2245700/71‑1 (MQ=255) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2066571/71‑1 (MQ=255) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:3652710/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:2481815/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:951577/70‑1 (MQ=9) cATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGa > 1:2581149/1‑56 (MQ=12) ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa < 1:3383326/67‑1 (MQ=255) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:4722556/68‑1 (MQ=12) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1279319/68‑1 (MQ=12) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3886370/70‑1 (MQ=255) cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:4050024/70‑1 (MQ=255) tcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATcc < 1:35234/66‑1 (MQ=12) cTGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:2917133/1‑68 (MQ=255) cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg < 1:3836516/71‑1 (MQ=255) tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt > 1:4297971/1‑67 (MQ=12) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCgg > 1:5179639/1‑44 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt < 1:153090/71‑1 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtta < 1:1167976/69‑2 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt > 1:4202839/3‑70 (MQ=12) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:5060615/62‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:1613290/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:303111/71‑1 (MQ=255) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:2598203/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:3861295/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCAGGAGTAATGAAACCGAATCCtttg < 1:1869002/60‑2 (MQ=12) gaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:5097356/1‑70 (MQ=255) aaGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3736081/55‑1 (MQ=255) aGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCAc < 1:4487975/71‑1 (MQ=255) tgtACGAACACATCTTTGCTGCCATCAGCCGGAGTa < 1:5235387/36‑1 (MQ=255) tgtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:5460608/39‑1 (MQ=255) tgtAAGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAccg < 1:1828006/45‑2 (MQ=2) tgtAAGAACACGTCATTGCTGCCGTCTTCCGGAGTAATGATACCGAATCCtt < 1:3246410/52‑1 (MQ=255) gtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg > 1:1775226/1‑38 (MQ=18) gtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATg > 1:1348652/1‑38 (MQ=18) gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:1027758/38‑1 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaaa < 1:4490316/40‑2 (MQ=17) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaa < 1:5693580/39‑1 (MQ=17) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa > 1:702603/1‑71 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa > 1:2964801/1‑71 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:4421765/1‑36 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:3011408/1‑36 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa > 1:4843779/1‑52 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa > 1:3796598/1‑52 (MQ=255) tACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAtttta > 1:4551257/1‑69 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:2164978/1‑46 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3856104/1‑46 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:1208086/1‑46 (MQ=255) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa > 1:2823655/1‑36 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa > 1:2849694/1‑36 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa > 1:2873384/1‑36 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGa > 1:3804661/1‑36 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa > 1:3629146/1‑61 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2731558/1‑62 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2622983/1‑62 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg > 1:4127071/1‑70 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg > 1:192844/1‑70 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc < 1:3081630/70‑1 (MQ=12) cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAcc < 1:4801092/71‑1 (MQ=255) gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt > 1:4944556/1‑71 (MQ=255) aaCACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcgtt < 1:4154567/71‑4 (MQ=12) aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga > 1:1525315/1‑38 (MQ=9) aaCACATCTTTACTACCATCAACAGGAGAAATAAAGccga > 1:1397473/1‑38 (MQ=9) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa < 1:3624785/71‑1 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa > 1:2078914/1‑71 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:3855277/61‑1 (MQ=255) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:4781149/71‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:5642545/51‑1 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:4205672/1‑36 (MQ=255) tttGGTGCCATCAGCCGGAGTAATGAAGCCAAAACCt > 1:5021466/1‑37 (MQ=255) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg < 1:1025835/41‑3 (MQ=12) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg < 1:4885998/40‑2 (MQ=12) tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg > 1:5654221/1‑63 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:3695205/69‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:2164328/68‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:3678572/68‑1 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:5137044/1‑71 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:3595011/1‑71 (MQ=255) tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:1711364/65‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt > 1:5457350/1‑71 (MQ=255) ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCa > 1:4018360/3‑42 (MQ=255) ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCa > 1:3968665/3‑42 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa > 1:5227390/1‑47 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt > 1:4610099/1‑64 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt > 1:3981886/1‑70 (MQ=255) ccGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:3480573/44‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc > 1:2053871/1‑46 (MQ=16) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa < 1:4366361/70‑4 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa < 1:3476175/70‑4 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca < 1:3136418/69‑3 (MQ=255) | AGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTG > minE/1211217‑1211354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |