| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,301 | A→G | 32.8% | F17F (TTT→TTC) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,301 | 0 | A | G | 32.8% | 186.9 / 113.3 | 134 | F17F (TTT→TTC) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base A (44/46); new base G (23/21); total (67/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
CCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTG > minE/1211233‑1211371 | cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:951577/70‑1 (MQ=9) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:5092732/70‑1 (MQ=9) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3805245/1‑71 (MQ=255) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:597120/1‑71 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa < 1:3383326/67‑1 (MQ=255) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1279319/68‑1 (MQ=12) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:4722556/68‑1 (MQ=12) ctcTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt < 1:2084618/69‑1 (MQ=9) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3886370/70‑1 (MQ=255) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGATGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:4280542/1‑70 (MQ=255) cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:4050024/70‑1 (MQ=255) cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg < 1:3836516/71‑1 (MQ=255) tGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa > 1:290164/1‑71 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGa > 1:4172713/1‑69 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt > 1:2843540/1‑71 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt < 1:523556/71‑1 (MQ=12) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt < 1:153090/71‑1 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtta < 1:1167976/69‑2 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt < 1:3159219/68‑1 (MQ=12) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:5060615/62‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:5539536/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:303111/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:1613290/71‑1 (MQ=255) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:3861295/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCAGGAGTAATGAAACCGAATCCtttg < 1:1869002/60‑2 (MQ=12) aGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCAc < 1:4487975/71‑1 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa > 1:702603/1‑71 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa > 1:2964801/1‑71 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:2181620/70‑1 (MQ=255) tACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAtttta > 1:4551257/1‑69 (MQ=255) gaCGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAAc > 1:893405/2‑47 (MQ=12) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3856104/1‑46 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:2164978/1‑46 (MQ=255) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa > 1:3629146/1‑61 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2622983/1‑62 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2731558/1‑62 (MQ=9) cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAcc < 1:4801092/71‑1 (MQ=255) aaCACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcgtt < 1:4154567/71‑4 (MQ=12) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa < 1:3971008/71‑1 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa > 1:2078914/1‑71 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:3855277/61‑1 (MQ=255) cgtCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCt > 1:3944241/3‑71 (MQ=12) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:4781149/71‑1 (MQ=255) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:1152790/71‑1 (MQ=255) aTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:4970200/71‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:5642545/51‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:4881688/60‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:5158342/60‑1 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:4205672/1‑36 (MQ=255) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg < 1:1025835/41‑3 (MQ=12) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg < 1:4885998/40‑2 (MQ=12) tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg > 1:5654221/1‑63 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:3695205/69‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:3678572/68‑1 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:3595011/1‑71 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:5137044/1‑71 (MQ=255) ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtt < 1:5041135/71‑1 (MQ=255) ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCt < 1:3743808/70‑1 (MQ=255) tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:402581/65‑1 (MQ=255) ccATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc > 1:1797797/1‑71 (MQ=255) aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa < 1:5384946/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt < 1:2535722/64‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt < 1:3575/64‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:1346808/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt > 1:5457350/1‑71 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt > 1:4610099/1‑64 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt > 1:3981886/1‑70 (MQ=255) ccGTAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc > 1:3683862/1‑46 (MQ=12) ccGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:3480573/44‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc > 1:2053871/1‑46 (MQ=16) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa < 1:3496766/70‑4 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa < 1:4366361/70‑4 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa < 1:3476175/70‑4 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca < 1:3627065/69‑3 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca < 1:3136418/69‑3 (MQ=255) ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa < 1:2943115/64‑1 (MQ=255) ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa < 1:3067404/71‑1 (MQ=255) ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa < 1:3113148/71‑1 (MQ=255) aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt > 1:3768443/1‑64 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:4339395/48‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:1822953/48‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa < 1:4087591/67‑1 (MQ=255) aaTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCttt < 1:3264398/48‑1 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa > 1:172183/1‑49 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa > 1:4980506/1‑49 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc < 1:2504374/71‑1 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc > 1:3092134/1‑71 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc < 1:3240707/71‑1 (MQ=255) tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:4771719/58‑1 (MQ=255) tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:5568382/58‑1 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:1909686/1‑64 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:2473115/1‑64 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:4413746/1‑64 (MQ=255) gCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCt > 1:2943908/1‑67 (MQ=255) ccAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTTcc < 1:2447139/51‑1 (MQ=255) cAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAt > 1:2456088/1‑70 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATc < 1:2119062/43‑1 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGcc < 1:3424188/49‑1 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt > 1:2056603/1‑51 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg < 1:3300760/71‑1 (MQ=255) | CCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTG > minE/1211233‑1211371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |