| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,258,382 | A→T | 25.0% | intergenic (+53/+112) | yecP → / ← torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,258,382 | 0 | A | T | 25.0% | 145.8 / 46.3 | 77 | intergenic (+53/+112) | yecP/torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (21/30); new base T (8/10); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
TGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGT > minE/1258314‑1258452 | tGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAcc > 1:3975321/1‑71 (MQ=255) gCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt > 1:4526559/1‑71 (MQ=255) ccGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTc < 1:1501724/71‑1 (MQ=255) gTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt < 1:927460/68‑1 (MQ=255) gTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa < 1:5475036/71‑1 (MQ=255) tAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa > 1:412870/1‑70 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatat < 1:5024919/71‑1 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:2229955/1‑70 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:2076606/1‑70 (MQ=255) ggTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatatat > 1:3478701/1‑71 (MQ=255) tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg < 1:1485836/71‑1 (MQ=255) tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg < 1:4844379/71‑1 (MQ=255) tGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg > 1:3480889/1‑69 (MQ=255) gTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg < 1:4004945/71‑1 (MQ=255) ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt < 1:2476714/54‑1 (MQ=255) ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata < 1:3528017/59‑1 (MQ=255) ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAc > 1:4767619/1‑71 (MQ=255) aCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata < 1:1564739/57‑1 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata < 1:5606571/55‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg > 1:3734384/1‑69 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGACCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:4526893/69‑1 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa < 1:1022153/49‑1 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa < 1:857020/49‑1 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg < 1:3870347/71‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt < 1:2538819/45‑1 (MQ=255) aTGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGcc > 1:1312535/1‑71 (MQ=2) tGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:887837/1‑48 (MQ=255) cggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTg < 1:3147214/70‑1 (MQ=255) gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata < 1:5157068/44‑1 (MQ=11) gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATaa < 1:3969092/71‑1 (MQ=2) cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata < 1:2385923/43‑1 (MQ=14) cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg > 1:2912678/1‑71 (MQ=9) cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg < 1:163709/71‑1 (MQ=9) cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt > 1:5601232/1‑69 (MQ=2) cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt > 1:1196526/1‑69 (MQ=2) gTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAatata > 1:2725210/1‑44 (MQ=11) gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg > 1:4938926/1‑62 (MQ=2) gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg > 1:88908/1‑62 (MQ=2) tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa < 1:2533891/48‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:3963067/57‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGcg > 1:5304301/1‑68 (MQ=9) aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGcg > 1:1237008/1‑68 (MQ=9) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa > 1:4045185/1‑37 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg > 1:4206099/1‑48 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg < 1:3962078/48‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg > 1:5130665/1‑48 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt < 1:4433128/71‑1 (MQ=255) aaCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:1345539/1‑69 (MQ=2) aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAg > 1:4359043/1‑71 (MQ=9) aCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:4030252/1‑68 (MQ=2) cGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata < 1:5378064/37‑1 (MQ=14) cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa > 1:5654816/1‑48 (MQ=255) cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa > 1:106423/1‑48 (MQ=255) ccTTATCCGGCCTACAAATCCTTGCGAATTCAata > 1:3253930/1‑35 (MQ=14) ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:1787019/1‑71 (MQ=255) ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:3335205/1‑71 (MQ=255) ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:63454/69‑1 (MQ=255) ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:955393/69‑1 (MQ=255) ttATCCGACCCACAAAGTCTTGCAAAATCAATAAATAGCAGGAACGATGTAGGCCTGATa > 1:935178/1‑60 (MQ=2) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:1791272/1‑69 (MQ=9) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa > 1:4474839/1‑69 (MQ=255) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:4121435/1‑70 (MQ=255) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:5182346/1‑70 (MQ=255) tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:4037079/43‑3 (MQ=14) tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:3424206/43‑3 (MQ=14) tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:3417786/43‑3 (MQ=14) tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:144553/43‑3 (MQ=14) tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCATCAg < 1:4437046/71‑1 (MQ=9) gCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCATCa > 1:3288905/1‑66 (MQ=9) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:3345577/55‑1 (MQ=255) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:920956/55‑1 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTAt < 1:4967192/38‑1 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa < 1:2978919/61‑1 (MQ=255) aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt < 1:1561115/54‑1 (MQ=255) aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt > 1:4506173/1‑71 (MQ=255) cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt < 1:2390062/44‑1 (MQ=255) cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt < 1:3539309/44‑1 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:959986/59‑1 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:3053980/59‑1 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt < 1:1847400/70‑1 (MQ=255) aaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGc > 1:1623060/1‑48 (MQ=255) aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt < 1:2336628/71‑1 (MQ=255) aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt < 1:4203399/71‑1 (MQ=255) | TGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGT > minE/1258314‑1258452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |