| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,258,418 | G→A | 22.5% | intergenic (+89/+76) | yecP → / ← torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,258,418 | 0 | G | A | 22.5% | 165.6 / 43.4 | 89 | intergenic (+89/+76) | yecP/torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (36/33); new base A (3/17); total (39/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTC > minE/1258348‑1258488 | ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg < 1:3870347/71‑1 (MQ=255) aTGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGcc > 1:1312535/1‑71 (MQ=2) cggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTg < 1:3147214/70‑1 (MQ=255) gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATaa < 1:3969092/71‑1 (MQ=2) cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg < 1:163709/71‑1 (MQ=9) cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg > 1:2912678/1‑71 (MQ=9) cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt > 1:1196526/1‑69 (MQ=2) cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt > 1:5601232/1‑69 (MQ=2) gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg > 1:88908/1‑62 (MQ=2) gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg > 1:4938926/1‑62 (MQ=2) aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGcg > 1:5304301/1‑68 (MQ=9) aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGcg > 1:1237008/1‑68 (MQ=9) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt < 1:4433128/71‑1 (MQ=255) aaCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:1345539/1‑69 (MQ=2) aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAg > 1:4359043/1‑71 (MQ=9) aCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:4030252/1‑68 (MQ=2) ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:3335205/1‑71 (MQ=255) ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:1787019/1‑71 (MQ=255) ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:63454/69‑1 (MQ=255) ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:955393/69‑1 (MQ=255) ttATCCGACCCACAAAGTCTTGCAAAATCAATAAATAGCAGGAACGATGTAGGCCTGATa > 1:935178/1‑60 (MQ=2) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:1791272/1‑69 (MQ=9) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa > 1:4474839/1‑69 (MQ=255) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:4121435/1‑70 (MQ=255) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:5182346/1‑70 (MQ=255) tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCATCAg < 1:4437046/71‑1 (MQ=9) gCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCATCa > 1:3288905/1‑66 (MQ=9) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:3345577/55‑1 (MQ=255) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:920956/55‑1 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa < 1:2978919/61‑1 (MQ=255) aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt < 1:1561115/54‑1 (MQ=255) aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt > 1:4506173/1‑71 (MQ=255) cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt < 1:3539309/44‑1 (MQ=255) cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt < 1:2390062/44‑1 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:959986/59‑1 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:3053980/59‑1 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt < 1:1847400/70‑1 (MQ=255) aaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGc > 1:1623060/1‑48 (MQ=255) aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt < 1:4203399/71‑1 (MQ=255) aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt < 1:2336628/71‑1 (MQ=255) ccTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGtt > 1:1082953/1‑71 (MQ=255) tGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt > 1:1692566/1‑69 (MQ=255) gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg > 1:2003473/1‑55 (MQ=255) gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg > 1:3310184/1‑55 (MQ=255) cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt < 1:3921158/71‑1 (MQ=255) cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt < 1:3633435/71‑1 (MQ=255) tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt > 1:2458677/1‑70 (MQ=255) tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt > 1:3643659/1‑70 (MQ=255) aagttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt > 1:2317224/4‑66 (MQ=9) aaTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt > 1:3091987/1‑58 (MQ=255) aaTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt > 1:831128/1‑58 (MQ=255) aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATaa > 1:4601760/1‑70 (MQ=255) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg < 1:4798301/53‑3 (MQ=9) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg < 1:4791809/53‑3 (MQ=9) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg < 1:1985576/53‑3 (MQ=9) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg < 1:5610084/53‑3 (MQ=9) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCtt < 1:5190587/56‑1 (MQ=9) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCtt < 1:1378907/56‑1 (MQ=9) ttCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa < 1:2500841/45‑1 (MQ=255) tCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGgctgct < 1:2949309/54‑1 (MQ=9) cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACg > 1:1236330/1‑58 (MQ=255) cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCt < 1:943738/70‑1 (MQ=255) aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc < 1:3025246/41‑1 (MQ=12) aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc < 1:5605311/41‑1 (MQ=12) aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc < 1:5136185/41‑1 (MQ=12) aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc < 1:5038193/41‑1 (MQ=12) aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt > 1:1525217/1‑58 (MQ=9) aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt > 1:3009687/1‑58 (MQ=9) atatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg < 1:2701218/71‑1 (MQ=255) atatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg < 1:357589/71‑1 (MQ=255) tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:785787/41‑1 (MQ=255) tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCa > 1:948591/1‑69 (MQ=255) atatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCa > 1:913594/1‑42 (MQ=255) tatTGGAGGGACTATGTAGGCCTGATAAGCATAGCGCATc > 1:3183619/1‑40 (MQ=255) tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc > 1:618650/1‑40 (MQ=255) tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc > 1:2292898/1‑40 (MQ=255) tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc > 1:5316275/1‑40 (MQ=255) atTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgcgc < 1:3789462/70‑1 (MQ=255) ttGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGccc > 1:5067378/1‑71 (MQ=255) cAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGtt < 1:3583970/71‑1 (MQ=255) gggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTc < 1:1467958/71‑1 (MQ=255) ggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTg < 1:2382131/49‑1 (MQ=255) tATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgc < 1:5310211/57‑1 (MQ=255) aTGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTa < 1:2472096/38‑1 (MQ=255) tGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgcgc < 1:4297408/71‑1 (MQ=255) tGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgc < 1:1599104/69‑1 (MQ=255) tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg < 1:2256842/39‑1 (MQ=11) tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg < 1:2115693/39‑1 (MQ=11) tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg < 1:4712131/39‑1 (MQ=11) tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg < 1:1362744/39‑1 (MQ=11) gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt < 1:5264968/41‑1 (MQ=14) gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt < 1:420357/41‑1 (MQ=14) ggCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTAtt < 1:1631156/71‑1 (MQ=255) gCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTc < 1:3030715/59‑1 (MQ=255) gCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTc < 1:3712108/59‑1 (MQ=255) gCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTc < 1:1320987/71‑1 (MQ=255) | GGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTC > minE/1258348‑1258488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |