| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,259,331 | A→C | 14.4% | A531A (GCT→GCG) ‡ | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,259,331 | 0 | A | C | 14.4% | 244.7 / 40.5 | 118 | A531A (GCT→GCG) ‡ | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (57/44); new base C (5/12); total (62/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
AGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAC > minE/1259262‑1259400 | ggTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGc > 1:4241530/1‑71 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:3357967/71‑1 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:3677550/71‑1 (MQ=255) gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:861176/71‑1 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc > 1:2259436/1‑64 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt < 1:618322/69‑1 (MQ=255) tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt < 1:5173178/69‑1 (MQ=255) aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc > 1:4216608/1‑71 (MQ=255) ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:2233876/61‑1 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt > 1:1757209/1‑64 (MQ=255) ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg > 1:4421697/1‑70 (MQ=255) cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac > 1:5262998/1‑70 (MQ=255) cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac > 1:5671875/1‑70 (MQ=255) aaGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa > 1:3155944/1‑71 (MQ=255) aactCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcac > 1:4615051/4‑68 (MQ=255) gATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata > 1:2673821/1‑71 (MQ=255) tCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAatat > 1:4082233/1‑70 (MQ=255) ggCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:1029714/49‑1 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacca < 1:4822424/62‑3 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:4185684/71‑3 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:3972096/71‑3 (MQ=255) aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:2588035/71‑3 (MQ=255) acacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa < 1:2597644/60‑1 (MQ=255) acGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt < 1:3570864/71‑1 (MQ=255) catCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTg > 1:316453/3‑71 (MQ=255) atCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:5234501/64‑3 (MQ=255) atCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat < 1:1929920/64‑3 (MQ=255) tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa < 1:579815/71‑1 (MQ=255) tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa < 1:1852202/71‑1 (MQ=255) tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa < 1:832803/71‑1 (MQ=255) tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa < 1:1980557/71‑1 (MQ=255) cAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa > 1:446779/1‑71 (MQ=255) aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGc > 1:269418/1‑49 (MQ=255) aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa > 1:1025002/1‑70 (MQ=255) aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt > 1:2360281/1‑71 (MQ=255) aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt > 1:283912/1‑71 (MQ=255) aaaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGAtta > 1:3493256/1‑63 (MQ=255) aaaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGAtta > 1:5047358/1‑63 (MQ=255) aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGcc < 1:4070980/36‑1 (MQ=255) aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt > 1:4862634/1‑70 (MQ=255) aaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTAATc < 1:52890/71‑1 (MQ=255) aaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt > 1:4330394/1‑64 (MQ=255) tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata > 1:2648123/1‑51 (MQ=255) tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt < 1:5148167/62‑1 (MQ=255) tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAcc > 1:1424967/1‑71 (MQ=255) gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg < 1:2205634/42‑1 (MQ=255) gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg < 1:2361834/42‑1 (MQ=255) gTTACGCGCTTAAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCgg > 1:3466036/1‑71 (MQ=255) cgcgCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCa < 1:498131/70‑1 (MQ=255) gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc < 1:5524540/71‑1 (MQ=255) gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc < 1:1981736/71‑1 (MQ=255) cgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATg > 1:5430121/1‑45 (MQ=255) gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc > 1:5050578/1‑61 (MQ=255) gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt > 1:54923/1‑71 (MQ=255) gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt > 1:84762/1‑71 (MQ=255) gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt > 1:189498/1‑71 (MQ=255) cTTCAAATTGCGGAGCAAGAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc < 1:4728641/60‑1 (MQ=255) cTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGc < 1:3094771/51‑1 (MQ=255) cTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt > 1:5582834/1‑52 (MQ=255) ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGt < 1:3300172/53‑1 (MQ=255) ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGt < 1:937645/53‑1 (MQ=255) ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc > 1:4572777/1‑70 (MQ=255) ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc > 1:5549815/1‑70 (MQ=255) tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa > 1:2859691/1‑37 (MQ=255) tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa > 1:3973667/1‑37 (MQ=255) tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa > 1:1566426/1‑37 (MQ=255) tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc > 1:856251/1‑69 (MQ=255) cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGc > 1:1447457/1‑41 (MQ=255) aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc > 1:1534788/1‑64 (MQ=255) aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt < 1:3778107/71‑1 (MQ=255) aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc < 1:2069629/71‑1 (MQ=255) aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCt < 1:3466968/39‑1 (MQ=255) aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCt < 1:4155405/39‑1 (MQ=255) aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt < 1:4320656/46‑1 (MQ=255) aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc < 1:5453119/70‑1 (MQ=255) ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAtac > 1:1033626/1‑50 (MQ=255) ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAt < 1:5566292/71‑1 (MQ=255) tGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt > 1:2781082/1‑71 (MQ=255) gCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt < 1:5179392/70‑1 (MQ=255) cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGt < 1:2151936/38‑1 (MQ=255) cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc > 1:250120/1‑71 (MQ=255) gAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa < 1:2915445/65‑1 (MQ=255) aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCt > 1:4602458/1‑71 (MQ=255) cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc > 1:1774422/1‑70 (MQ=255) cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc > 1:3774467/1‑70 (MQ=255) aaCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa > 1:1230307/1‑70 (MQ=255) aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt > 1:5518979/1‑62 (MQ=255) aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt > 1:4889175/1‑62 (MQ=255) aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa < 1:5628727/71‑1 (MQ=255) aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa < 1:4148397/71‑1 (MQ=255) aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa > 1:3573577/1‑71 (MQ=255) aCAGCCTGCTTCATCGGCACAATATGCTGGTAGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa > 1:4892277/1‑71 (MQ=255) aCAGCCTGCTTCATAGGCACAATATGCTGGTTGATGt > 1:5093352/1‑37 (MQ=38) cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt < 1:195285/57‑1 (MQ=255) cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt < 1:878753/57‑1 (MQ=255) cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc < 1:658400/71‑1 (MQ=255) cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc < 1:1167206/71‑1 (MQ=255) cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc > 1:5322952/1‑71 (MQ=255) aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc > 1:1588531/1‑70 (MQ=255) aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc > 1:4465805/1‑70 (MQ=255) | AGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAC > minE/1259262‑1259400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |