| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 222,997 | T→A | 47.8% | intergenic (+52/‑128) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 222,997 | 0 | T | A | 47.8% | 50.7 / 91.3 | 71 | intergenic (+52/‑128) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base T (18/11); new base A (15/16); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAAT > minE/222927‑223067 | aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:3333644/71‑1 (MQ=255)aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:1090087/71‑1 (MQ=255)aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct > 1:155804/1‑71 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg < 1:764669/71‑1 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 1:1296964/1‑71 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 1:3852053/1‑71 (MQ=255) tGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctg < 1:1196182/71‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg < 1:3505789/70‑1 (MQ=255) agtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCt < 1:1655151/71‑1 (MQ=255) cTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc > 1:434380/1‑71 (MQ=255) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGcagatgcct < 1:2467046/50‑1 (MQ=9) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:3991590/70‑1 (MQ=9) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:4325091/70‑1 (MQ=9) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa < 1:3968490/71‑1 (MQ=255) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa > 1:4039942/1‑71 (MQ=255) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:2766014/1‑70 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:3118110/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4582333/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4683535/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGAATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:834604/70‑1 (MQ=2) gCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTa > 1:4619478/1‑70 (MQ=11) cGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt < 1:2226868/64‑1 (MQ=14) ggATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGcagatgcctgatgc < 1:1756742/50‑1 (MQ=11) ggATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGcagatgcctgatgc < 1:422928/50‑1 (MQ=11) ggATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:1063915/1‑68 (MQ=14) ggACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTATc < 1:3573976/67‑1 (MQ=9) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:4350560/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:824945/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:5554197/1‑69 (MQ=11) gATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:1701772/44‑1 (MQ=255) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctga > 1:2609340/1‑45 (MQ=255) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctga > 1:3616525/1‑45 (MQ=255) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctga > 1:1805980/1‑45 (MQ=255) tAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGcc < 1:3814930/71‑1 (MQ=11) tAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 1:4290447/1‑37 (MQ=255) aggcGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTa < 1:1875605/68‑1 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:75157/67‑1 (MQ=11) aGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:3666333/1‑71 (MQ=255) aGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:989136/1‑71 (MQ=255) ggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc < 1:5436686/69‑1 (MQ=11) gTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaaa > 1:1943052/1‑71 (MQ=14) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATg > 1:1076548/1‑63 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGc > 1:2237443/1‑64 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa > 1:2742367/1‑71 (MQ=255) tttATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATc < 1:3095830/59‑1 (MQ=11) ttcaCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAt < 1:316021/68‑1 (MQ=11) ttATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaaact > 1:187324/1‑69 (MQ=11) ttACGCCGCATCCGGCATTTGTGCGATGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:2157744/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3034541/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:5551855/1‑71 (MQ=255) tACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGc > 1:5012285/1‑43 (MQ=14) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:3545990/2‑69 (MQ=11) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttc > 1:4035262/2‑69 (MQ=11) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttc > 1:2380082/2‑69 (MQ=11) cgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttct < 1:3513868/70‑4 (MQ=11) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc > 1:4984833/1‑71 (MQ=255) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc < 1:5228399/71‑1 (MQ=255) cgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcg < 1:4007667/71‑1 (MQ=255) cgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt > 1:557805/1‑46 (MQ=14) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:3970778/1‑71 (MQ=255) gcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACa < 1:1360600/60‑1 (MQ=11) cATCCGGCA‑CTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTAcg < 1:1850183/58‑2 (MQ=14) tCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttc > 1:1944324/1‑60 (MQ=11) cATTTGTGCGCTGATGCCTGATGCGACGCTGCCGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:1816658/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:2460913/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:374981/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa < 1:1381827/71‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaa < 1:1505484/51‑1 (MQ=255) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:3307470/41‑1 (MQ=14) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:1633189/41‑1 (MQ=14) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTAt < 1:599282/38‑1 (MQ=14) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTAt < 1:4718543/38‑1 (MQ=14) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:3118635/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:2547384/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc > 1:2958194/1‑71 (MQ=255) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1661702/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1834998/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:4043461/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:2721273/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3473134/1‑45 (MQ=11) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3616852/1‑45 (MQ=11) gtgCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:4971846/1‑50 (MQ=14) tgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGa < 1:2791128/71‑1 (MQ=255) cgcTGATGCCTGATGTGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa < 1:2414668/70‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1277350/67‑1 (MQ=255) gcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:14595/71‑1 (MQ=255) ctgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:4647595/70‑1 (MQ=255) tgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataat > 1:3498067/1‑71 (MQ=255) | AAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAAT > minE/222927‑223067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |