Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,259,379 C→G 16.7% T515T (ACG→ACC torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,259,3790CG16.7% 436.3 / 71.1 185T515T (ACG→ACCtorZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (70/84);  new base G (14/17);  total (84/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 189 total aligned reads displayed.

GCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTC  >  minE/1259309‑1259448
                                                                      |                                                                     
gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                                       <  1:5524540/71‑1 (MQ=255)
gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                                       <  1:1981736/71‑1 (MQ=255)
  gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt                                                                     >  1:84762/1‑71 (MQ=255)
  gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt                                                                     >  1:189498/1‑71 (MQ=255)
    ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc                                                                    >  1:4572777/1‑70 (MQ=255)
       aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                                       >  1:1534788/1‑64 (MQ=255)
         aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                                               <  1:5453119/70‑1 (MQ=255)
          ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAt                                                             <  1:5566292/71‑1 (MQ=255)
            gCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                                                            <  1:5179392/70‑1 (MQ=255)
             cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc                                                          >  1:250120/1‑71 (MQ=255)
               gAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                                                              <  1:2915445/65‑1 (MQ=255)
                aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCt                                                       >  1:4602458/1‑71 (MQ=255)
                  cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc                                                      >  1:3774467/1‑70 (MQ=255)
                    aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                                                            >  1:4889175/1‑62 (MQ=255)
                    aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                                                            >  1:5518979/1‑62 (MQ=255)
                    aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                                                   >  1:4710690/1‑71 (MQ=255)
                    aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                                                   <  1:4148397/71‑1 (MQ=255)
                    aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                                                   >  1:3573577/1‑71 (MQ=255)
                     cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                  >  1:5322952/1‑71 (MQ=255)
                      aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                  >  1:1588531/1‑70 (MQ=255)
                       gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                                                              >  1:3182833/1‑57 (MQ=255)
                       gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGa                                                <  1:898039/71‑1 (MQ=255)
                        cttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGag                                               >  1:1603803/3‑70 (MQ=255)
                        ccTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa                                                    <  1:320250/66‑1 (MQ=255)
                         ttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtt                                                            <  1:1105413/56‑1 (MQ=255)
                         ttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCaaaag                                                 <  1:225313/67‑3 (MQ=255)
                         ttGCTTCATCGGCACCAGATGCTGAATACTGTAATCACCGGTCATGGTGAGATCATTACGCTCaaaag                                                 <  1:3971516/67‑3 (MQ=255)
                         cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                              <  1:2590335/71‑1 (MQ=255)
                          tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                             >  1:2879637/1‑71 (MQ=255)
                           gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            >  1:5045382/1‑71 (MQ=255)
                            cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            <  1:2951810/70‑1 (MQ=255)
                            cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtgg                                           <  1:3553840/71‑1 (MQ=255)
                             ttCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtt                                                            >  1:3875618/1‑53 (MQ=255)
                             ttCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtt                                                            >  1:3026116/1‑53 (MQ=255)
                             ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCATAc                                                  <  1:936273/63‑1 (MQ=255)
                             ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                                                  <  1:5655367/63‑1 (MQ=255)
                              tCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt                                          >  1:4743670/1‑70 (MQ=255)
                              tCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt                                          >  1:3802995/1‑70 (MQ=255)
                               cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCAtta                                                           >  1:1386202/1‑51 (MQ=255)
                               cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                         <  1:1185271/70‑2 (MQ=255)
                                aTCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtcg                                        >  1:3031055/1‑68 (MQ=255)
                                aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                       <  1:820891/71‑1 (MQ=255)
                                aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                       <  1:3427176/71‑1 (MQ=255)
                                aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                       <  1:2179754/71‑1 (MQ=255)
                                aTCGGCACAATATGCTGGTTGCTGTAATCACCCGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                       <  1:5504730/71‑1 (MQ=255)
                                 tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                                               <  1:186794/46‑1 (MQ=255)
                                 tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                                     <  1:3467363/56‑1 (MQ=255)
                                 tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                                     <  1:5094596/56‑1 (MQ=255)
                                   ggCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTacg                                                         >  1:1250668/1‑50 (MQ=255)
                                   ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCg                                                                      >  1:1056409/1‑37 (MQ=255)
                                   ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCg                                                                      >  1:1657379/1‑37 (MQ=255)
                                   ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                    >  1:4789844/1‑71 (MQ=255)
                                   ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                    >  1:523185/1‑71 (MQ=255)
                                       caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                 <  1:490495/70‑1 (MQ=255)
                                        aaTATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa                                                    <  1:3048315/50‑1 (MQ=255)
                                        aaTATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATa                               <  1:5124843/71‑1 (MQ=255)
                                         atatGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATAc                              <  1:3534282/71‑1 (MQ=255)
                                          tatGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                 <  1:4404097/51‑1 (MQ=255)
                                           atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                           <  1:2164032/56‑3 (MQ=255)
                                           atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                           <  1:1144911/56‑3 (MQ=255)
                                           atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                           <  1:2080798/56‑3 (MQ=255)
                                            tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                     <  1:3453030/61‑1 (MQ=255)
                                             gCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                         >  1:1265162/1‑55 (MQ=255)
                                             gCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                         >  1:4088047/1‑55 (MQ=255)
                                              cTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt                                   >  1:1988109/1‑61 (MQ=255)
                                                ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            <  1:4555315/50‑1 (MQ=255)
                                                ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            <  1:5403246/50‑1 (MQ=255)
                                                ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            <  1:5346000/50‑1 (MQ=255)
                                                ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtgg                                           >  1:1739802/1‑51 (MQ=255)
                                                  ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                 <  1:2762776/59‑1 (MQ=255)
                                                  ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGc                     >  1:1784590/1‑71 (MQ=255)
                                                  ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGc                     <  1:686288/71‑1 (MQ=255)
                                                     cTGTAATCACCGGTCATCGTCAGGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                         <  1:1289311/64‑1 (MQ=255)
                                                     cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                         <  1:2739635/64‑1 (MQ=255)
                                                     cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                  <  1:444180/71‑1 (MQ=255)
                                                      tGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGat                           <  1:4050599/61‑1 (MQ=255)
                                                        tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                <  1:3663348/54‑1 (MQ=255)
                                                        tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                <  1:862157/54‑1 (MQ=255)
                                                        tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtgt                 <  1:4390851/69‑1 (MQ=255)
                                                         aaTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                         >  1:4503763/1‑60 (MQ=255)
                                                         aaTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTg              >  1:1209252/1‑71 (MQ=255)
                                                          aTCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGttt               <  1:5589473/69‑1 (MQ=255)
                                                          aTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                              >  1:3920167/1‑38 (MQ=255)
                                                          aTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGAAATCTGCGTGATTgg             >  1:4029971/1‑71 (MQ=255)
                                                           tCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTgg             >  1:2713331/1‑70 (MQ=255)
                                                           tCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgc            <  1:4492868/71‑1 (MQ=255)
                                                            cACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            >  1:5627515/1‑38 (MQ=255)
                                                            cACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            >  1:2396140/1‑38 (MQ=255)
                                                            cACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                            >  1:2327770/1‑38 (MQ=255)
                                                             aCCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgct           >  1:4803783/1‑70 (MQ=255)
                                                                ggTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATa                               >  1:2897523/1‑47 (MQ=255)
                                                                 gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                             <  1:4284283/48‑1 (MQ=255)
                                                                 gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCtgctgc      <  1:5486043/71‑1 (MQ=255)
                                                                   cATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                     >  1:764864/1‑38 (MQ=255)
                                                                    aTCGTCAATTCATTGCGCTCAAACGATGTGGTGATCGGt                                   >  1:5305472/1‑39 (MQ=255)
                                                                    aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                  >  1:4650951/1‑40 (MQ=255)
                                                                    aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                  >  1:4120518/1‑40 (MQ=255)
                                                                     tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc  <  1:3350016/71‑1 (MQ=255)
                                                                     tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc  <  1:2384150/71‑1 (MQ=255)
                                                                      cGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc  >  1:1160132/1‑70 (MQ=255)
                                                                      |                                                                     
GCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTC  >  minE/1259309‑1259448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: