| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 223,016 | G→A | 20.5% | intergenic (+71/‑109) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 223,016 | 0 | G | A | 20.5% | 261.0 / 53.0 | 122 | intergenic (+71/‑109) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base G (47/50); new base A (12/13); total (59/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGA > minE/222948‑223086 | cTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc > 1:434380/1‑71 (MQ=255) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:3991590/70‑1 (MQ=9) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:4325091/70‑1 (MQ=9) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa < 1:3968490/71‑1 (MQ=255) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa > 1:4039942/1‑71 (MQ=255) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:2766014/1‑70 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:3118110/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4582333/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4683535/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGAATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:834604/70‑1 (MQ=2) gCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTa > 1:4619478/1‑70 (MQ=11) cGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt < 1:2226868/64‑1 (MQ=14) ggATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:1063915/1‑68 (MQ=14) ggACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTATc < 1:3573976/67‑1 (MQ=9) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:4350560/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:824945/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:5554197/1‑69 (MQ=11) tAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGcc < 1:3814930/71‑1 (MQ=11) aggcGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTa < 1:1875605/68‑1 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:75157/67‑1 (MQ=11) aGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:3666333/1‑71 (MQ=255) ggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc < 1:5436686/69‑1 (MQ=11) gTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaaa > 1:1943052/1‑71 (MQ=14) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATg > 1:1076548/1‑63 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa > 1:2742367/1‑71 (MQ=255) tttATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATc < 1:3095830/59‑1 (MQ=11) ttcaCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAt < 1:316021/68‑1 (MQ=11) ttATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaaact > 1:187324/1‑69 (MQ=11) ttACGCCGCATCCGGCATTTGTGCGATGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:2157744/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:5551855/1‑71 (MQ=255) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:3545990/2‑69 (MQ=11) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttc > 1:2380082/2‑69 (MQ=11) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc < 1:5228399/71‑1 (MQ=255) cgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcg < 1:4007667/71‑1 (MQ=255) cgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt > 1:557805/1‑46 (MQ=14) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:3970778/1‑71 (MQ=255) gcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACa < 1:1360600/60‑1 (MQ=11) cATCCGGCA‑CTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTAcg < 1:1850183/58‑2 (MQ=14) tCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttc > 1:1944324/1‑60 (MQ=11) cATTTGTGCGCTGATGCCTGATGCGACGCTGCCGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:1816658/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa < 1:1381827/71‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaa < 1:1505484/51‑1 (MQ=255) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:1633189/41‑1 (MQ=14) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:3307470/41‑1 (MQ=14) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTAt < 1:599282/38‑1 (MQ=14) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTAt < 1:4718543/38‑1 (MQ=14) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:2547384/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:3118635/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc > 1:2958194/1‑71 (MQ=255) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1661702/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:2721273/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:4043461/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3473134/1‑45 (MQ=11) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3616852/1‑45 (MQ=11) gtgCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:4971846/1‑50 (MQ=14) tgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGa < 1:2791128/71‑1 (MQ=255) cgcTGATGCCTGATGTGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa < 1:2414668/70‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1277350/67‑1 (MQ=255) gcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:14595/71‑1 (MQ=255) cctatgcctgatgcgacGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:1427658‑M1/58‑20 (MQ=255) tgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataat > 1:3498067/1‑71 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:4228679‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:1437379‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta > 1:875263‑M1/3‑41 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:1004805‑M1/2‑40 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:2101907‑M1/2‑40 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:1903227‑M1/2‑40 (MQ=255) gatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATg > 1:1984470/1‑71 (MQ=255) atgcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc > 1:2946535‑M1/1‑39 (MQ=255) gcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTCTACGCGAACCGTAGGc > 1:4099538/1‑59 (MQ=255) gcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtat < 1:2568832‑M1/53‑17 (MQ=255) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3815614/71‑1 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg > 1:88171/1‑47 (MQ=255) tgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:2198426/69‑1 (MQ=255) tgatgcGACGCTAACGCGTCTTATCATGCCTACAAATcgc < 1:5430110/40‑3 (MQ=14) gatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:5660260‑M1/52‑20 (MQ=255) gatgcGACGCTGAAGCGTCTTATCAAGCCTACAtagcactgccacgtatgtttacaccgcatccggcata > 1:818729‑M1/1‑33 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCa > 1:1062898/1‑69 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcg > 1:3836249/1‑71 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcac > 1:4033205‑M1/1‑32 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:4048171‑M1/69‑39 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:2484138‑M1/69‑39 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:2048094‑M1/69‑39 (MQ=255) gcGACGCTAACGCGTCTTATCAGCCCTACAAATTCTCACGCCATCCGTAGGCCGGATAAGGCGTTCAcgcc > 1:3373977/1‑71 (MQ=9) cGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGt < 1:421325/47‑1 (MQ=255) cGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGt < 1:2338056/47‑1 (MQ=255) gACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaaca > 1:1857692‑M1/1‑28 (MQ=255) aCGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:5142139/50‑1 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaa < 1:1281470‑M1/68‑42 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaa < 1:3098024‑M1/68‑42 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatg < 1:1429198‑M1/44‑18 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatg < 1:769342‑M1/44‑18 (MQ=255) cGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCAt > 1:4647210/1‑71 (MQ=255) gCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg > 1:4238763/1‑37 (MQ=255) cTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgc > 1:1281187/1‑64 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcg < 1:3770558‑M1/71‑48 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcg < 1:4858968‑M1/71‑48 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:2391419‑M1/63‑40 (MQ=255) tGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1671084/46‑1 (MQ=255) gACGCGACTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGa > 1:732620/1‑71 (MQ=255) | CTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGA > minE/222948‑223086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |