| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,259,599 | T→G | 5.7% | K442T (AAA→ACA) ‡ | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,259,599 | 0 | T | G | 5.7% | 233.1 / 6.5 | 87 | K442T (AAA→ACA) ‡ | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (54/28); new base G (3/2); total (57/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TTACCGCCACCCGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCAT > minE/1259530‑1259665 | ttACCGCCACCCGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATAttt < 1:2548149/71‑1 (MQ=255) tACCGCCACCCGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTg > 1:4060732/1‑71 (MQ=255) aCCGCCACCCGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTgc > 1:4235548/1‑71 (MQ=255) cGCCACCCGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTgccg > 1:1185557/1‑71 (MQ=255) cccGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAgg < 1:3596924/71‑1 (MQ=255) ccGCCAACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAgg < 1:2294095/70‑1 (MQ=255) cGCCCACCGGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAt < 1:525012/71‑1 (MQ=255) cccACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAttt > 1:5520131/1‑71 (MQ=255) ccaGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTc > 1:3812701/1‑69 (MQ=255) caGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAg > 1:4086117/1‑71 (MQ=255) aGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAgc > 1:287859/1‑71 (MQ=255) aGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAgc > 1:2913737/1‑71 (MQ=255) aGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAgc > 1:908143/1‑71 (MQ=255) aGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAg > 1:1742802/1‑70 (MQ=255) aGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAg > 1:1473343/1‑70 (MQ=255) gATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAgcg < 1:4043687/71‑1 (MQ=255) aTCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTgcc < 1:5461144/53‑1 (MQ=255) tCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAtttt > 1:972954/1‑63 (MQ=255) cATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAtt > 1:608410/1‑60 (MQ=255) cATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAtt > 1:874394/1‑60 (MQ=255) cATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAtt > 1:2568848/1‑60 (MQ=255) cATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCAt > 1:3971528/1‑71 (MQ=255) cATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCAt > 1:2478823/1‑71 (MQ=255) cATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCAt > 1:2593680/1‑71 (MQ=255) aTTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATc < 1:5390111/71‑1 (MQ=255) aTTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATc < 1:1507028/71‑1 (MQ=255) ttttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTc > 1:5292358/1‑61 (MQ=255) ttttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATc > 1:3886777/1‑70 (MQ=255) tttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga > 1:3963912/1‑71 (MQ=255) ttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:2727893/1‑71 (MQ=255) ttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:1933358/1‑71 (MQ=255) tGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac < 1:4129135/70‑1 (MQ=255) atatTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga < 1:3541201/70‑1 (MQ=255) ttCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATAc < 1:3431187/70‑1 (MQ=255) tCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTg > 1:1226866/1‑38 (MQ=255) tCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga < 1:1494828/63‑1 (MQ=255) cGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAg > 1:4405822/1‑44 (MQ=255) ggATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGc < 1:750469/71‑1 (MQ=255) tACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTcc < 1:3220034/48‑1 (MQ=255) tttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATa < 1:1846398/59‑1 (MQ=255) tttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATa < 1:1396129/59‑1 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:5074405/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:4249166/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:3731956/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:3849606/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:3067841/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGaa > 1:2301660/1‑71 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga > 1:1171895/1‑52 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCaa > 1:4416331/1‑64 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:5262237/1‑71 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:567388/1‑71 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:850539/1‑71 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGAt < 1:4406132/36‑1 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCATCAGGAATc > 1:168942/1‑71 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt < 1:4522051/70‑1 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATc > 1:3700420/1‑71 (MQ=255) ttCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:2298950/1‑53 (MQ=255) ttCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATc < 1:1679952/48‑1 (MQ=255) ttCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATc < 1:4758252/48‑1 (MQ=255) ttCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGa < 1:5199668/67‑1 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga > 1:2381079/1‑48 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga < 1:3090719/48‑1 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:429945/1‑67 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:5448335/1‑67 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGcc > 1:2998410/1‑71 (MQ=255) tttACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATc < 1:3247156/45‑1 (MQ=255) ttACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt > 1:128213/1‑71 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt > 1:1514098/1‑70 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:4964833/70‑1 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:340776/70‑1 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:4261229/70‑1 (MQ=255) ccGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAggg > 1:5087717/1‑59 (MQ=255) ccGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAggg > 1:3145899/1‑59 (MQ=255) ccGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGAttt > 1:1695375/1‑62 (MQ=255) aTGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCg < 1:5195118/70‑1 (MQ=255) tGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGc > 1:1056701/1‑70 (MQ=255) tGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGc > 1:4152849/1‑70 (MQ=255) gCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:4065946/1‑37 (MQ=255) gCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:2703606/1‑37 (MQ=255) gCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:5494210/1‑37 (MQ=255) ttGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGAt < 1:5642868/53‑1 (MQ=255) tGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATa < 1:3528062/39‑1 (MQ=255) tGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGAt < 1:5274536/53‑1 (MQ=255) atatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatca < 1:5301244/70‑1 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTc > 1:4371361/1‑61 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTc > 1:3113091/1‑61 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTc > 1:4898602/1‑61 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat > 1:489677/1‑70 (MQ=255) tttGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGaa > 1:4554316/1‑47 (MQ=255) tttGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGa < 1:1910830/46‑1 (MQ=255) | TTACCGCCACCCGCCCACCAGATCATTTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCAT > minE/1259530‑1259665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |