| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,259,625 | G→A | 7.2% | V433V (GTC→GTT) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,259,625 | 0 | G | A | 7.2% | 201.4 / 4.1 | 69 | V433V (GTC→GTT) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (33/31); new base A (3/2); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCG > minE/1259556‑1259695 | tttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga > 1:3963912/1‑71 (MQ=255) ttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:2727893/1‑71 (MQ=255) ttGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:1933358/1‑71 (MQ=255) tGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac < 1:4129135/70‑1 (MQ=255) atatTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga < 1:3541201/70‑1 (MQ=255) ttCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATAc < 1:3431187/70‑1 (MQ=255) tCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga < 1:1494828/63‑1 (MQ=255) ggATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGc < 1:750469/71‑1 (MQ=255) tttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATa < 1:1396129/59‑1 (MQ=255) tttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATa < 1:1846398/59‑1 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:4249166/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:5074405/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:3731956/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:3067841/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:3849606/1‑56 (MQ=255) ttGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGaa > 1:2301660/1‑71 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga > 1:1171895/1‑52 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCaa > 1:4416331/1‑64 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:567388/1‑71 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:5262237/1‑71 (MQ=255) tGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:850539/1‑71 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCATCAGGAATc > 1:168942/1‑71 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt < 1:4522051/70‑1 (MQ=255) gTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATc > 1:3700420/1‑71 (MQ=255) ttCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgacga > 1:2298950/1‑53 (MQ=255) ttCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGa < 1:5199668/67‑1 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga < 1:3090719/48‑1 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATcga > 1:2381079/1‑48 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:5448335/1‑67 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:429945/1‑67 (MQ=255) cTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGcc > 1:2998410/1‑71 (MQ=255) ttACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt > 1:128213/1‑71 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:4261229/70‑1 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:340776/70‑1 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:4964833/70‑1 (MQ=255) tACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt > 1:1514098/1‑70 (MQ=255) ccGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAggg > 1:3145899/1‑59 (MQ=255) ccGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAggg > 1:5087717/1‑59 (MQ=255) ccGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGAttt > 1:1695375/1‑62 (MQ=255) aTGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCg < 1:5195118/70‑1 (MQ=255) tGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGc > 1:4152849/1‑70 (MQ=255) tGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGc > 1:1056701/1‑70 (MQ=255) gCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:4065946/1‑37 (MQ=255) gCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:5494210/1‑37 (MQ=255) gCTGATATTTGCCGCCAGGATTTTCCAGCGCATcgac > 1:2703606/1‑37 (MQ=255) ttGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGAt < 1:5642868/53‑1 (MQ=255) tGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATa < 1:3528062/39‑1 (MQ=255) tGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGAt < 1:5274536/53‑1 (MQ=255) atatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatca < 1:5301244/70‑1 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTc > 1:3113091/1‑61 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTc > 1:4371361/1‑61 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTc > 1:4898602/1‑61 (MQ=255) tatTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat > 1:489677/1‑70 (MQ=255) tttGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGaa > 1:4554316/1‑47 (MQ=255) tttGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGa < 1:1910830/46‑1 (MQ=255) tGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCg > 1:332939/1‑71 (MQ=255) ccgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGc < 1:476519/58‑1 (MQ=255) cgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat > 1:1364440/1‑63 (MQ=255) cgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGcc < 1:4989511/71‑1 (MQ=255) cgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGcc > 1:82557/1‑71 (MQ=255) cgccAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGcc < 1:1653200/71‑1 (MQ=255) aGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGcc < 1:4784928/43‑1 (MQ=255) aGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGc > 1:2855123/1‑71 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGa < 1:3863570/35‑1 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGa < 1:1339194/35‑1 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:5176533/43‑1 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:5182770/43‑1 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:1315152/43‑1 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGt < 1:4889252/43‑1 (MQ=255) gATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCatcatc < 1:4849533/58‑1 (MQ=255) aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTc < 1:782481/43‑1 (MQ=255) aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTc < 1:2801507/43‑1 (MQ=255) aTTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTgg < 1:210047/71‑1 (MQ=255) ttttCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:4680944/1‑35 (MQ=255) ttttCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:1220249/1‑35 (MQ=255) ttttCCAGCGCATCGACGATACGGGCAACAGGAAt > 1:1115151/1‑35 (MQ=255) ttttCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAg > 1:1573795/1‑58 (MQ=255) ttttCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAg > 1:4939809/1‑58 (MQ=255) tttCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat < 1:634346/54‑1 (MQ=255) tttCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat < 1:895565/54‑1 (MQ=255) tttCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat < 1:3976185/54‑1 (MQ=255) tttCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCcatcat < 1:1938859/54‑1 (MQ=255) tCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCa > 1:3915666/1‑40 (MQ=255) tCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCa > 1:1238459/1‑40 (MQ=255) aGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTcc > 1:1461784/1‑71 (MQ=255) aGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTcc > 1:1176889/1‑71 (MQ=255) aGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTc > 1:4970305/1‑70 (MQ=255) gacgaTACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATcg > 1:4138658/1‑71 (MQ=255) | TTTGATATTCGGATACGTTTGTTCTTTACCGTTATGCTGATATTTGCCGCCAGGATTTTCCAGCGCATCGACGATACGGGCAACAGGAATCGCCGTCATTCCGCCATCATCAGCGGCCTCGCTGGCATGTCCGGCAATCG > minE/1259556‑1259695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |