| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,315 | A→C | 13.8% | I203M (ATT→ATG) ‡ | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,315 | 0 | A | C | 13.8% | 196.9 / 30.8 | 94 | I203M (ATT→ATG) ‡ | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (39/42); new base C (9/4); total (48/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTG > minE/1260246‑1260385 | gCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGaa > 1:5349198/1‑71 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGaa < 1:1999359/70‑1 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGa < 1:3687793/69‑1 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTACCAGaa < 1:733554/70‑1 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCGAGAATc < 1:692/69‑1 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCa < 1:4729602/70‑1 (MQ=255) cAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGc < 1:3450325/71‑1 (MQ=255) cAATTTTCAGCGTATTGAGTGGGTTAGCACTCCACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGc > 1:4677745/1‑71 (MQ=255) aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGcc < 1:1686427/71‑1 (MQ=255) aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGcc < 1:3640974/71‑1 (MQ=255) aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGcc < 1:5603068/71‑1 (MQ=255) tttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGGTGTTTTCCAGAATCAGCGGCCa < 1:2295390/70‑1 (MQ=255) tttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGc < 1:145547/68‑1 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:4674443/70‑1 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag > 1:5569303/1‑69 (MQ=255) aaGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag > 1:1400898/1‑69 (MQ=255) aGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCATAATCAGCGGCCAGGAGGTc > 1:3443078/1‑71 (MQ=255) aGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTc > 1:711866/1‑71 (MQ=255) aGGTATTCAGCGGGTTCATTCCCCACAGCACCAAAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGt < 1:2277717/70‑1 (MQ=255) tATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc < 1:4918965/71‑1 (MQ=255) aTTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc > 1:4336443/1‑70 (MQ=255) ttCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgctg < 1:5566174/71‑1 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGa > 1:1584633/1‑46 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag > 1:3571836/1‑58 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt > 1:4141498/1‑70 (MQ=255) aGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAAt > 1:375176/1‑47 (MQ=255) gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:3114892/57‑1 (MQ=255) gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:1378839/57‑1 (MQ=255) gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa < 1:5631559/71‑1 (MQ=255) cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGt > 1:3354627/1‑60 (MQ=255) cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa > 1:1336383/1‑70 (MQ=255) ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag > 1:3723437/1‑56 (MQ=255) ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag > 1:3823476/1‑56 (MQ=255) ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag > 1:1223074/1‑56 (MQ=255) ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATac < 1:4160766/71‑1 (MQ=255) ggTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATAAGCGGCCaggag > 1:3482302/1‑56 (MQ=255) gTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATaca < 1:352442/71‑1 (MQ=255) tCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACAcc > 1:1785152/1‑71 (MQ=255) aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa < 1:3573935/63‑1 (MQ=255) aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATaca > 1:2900489/1‑67 (MQ=255) aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATaca > 1:5071132/1‑67 (MQ=255) aTTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:4038360/1‑65 (MQ=255) ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGc < 1:4256170/44‑1 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAg < 1:677708/39‑1 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:4327800/1‑71 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:4819010/1‑71 (MQ=255) cccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCg > 1:5255032/1‑39 (MQ=255) cccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCa > 1:2272889/1‑43 (MQ=255) ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg < 1:5527836/71‑1 (MQ=255) ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg < 1:4607476/71‑1 (MQ=255) cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaac < 1:1399712/62‑1 (MQ=255) cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttcac > 1:2175164/1‑65 (MQ=255) caGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaact > 1:4406122/1‑60 (MQ=255) aGCACCACGACATCGCTATGTTCCAGCACCAGCGGTCAACTGGTCTGCTGTTGATaaa > 1:4046215/1‑56 (MQ=255) aGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa > 1:5234272/1‑56 (MQ=255) aGCACCAAGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa > 1:4937699/1‑56 (MQ=255) gCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc > 1:4129664/1‑70 (MQ=255) caccacGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa > 1:3883834/1‑54 (MQ=255) ccacAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt < 1:5568185/47‑1 (MQ=255) ccacAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt < 1:1189137/47‑1 (MQ=255) cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa < 1:3424540/61‑1 (MQ=255) cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca < 1:657866/71‑1 (MQ=255) acaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac < 1:1199900/71‑1 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACctc < 1:2356329/55‑1 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2621935/1‑71 (MQ=255) aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt > 1:745218/1‑71 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca < 1:5497130/69‑1 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg > 1:1607392/1‑71 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg < 1:1657138/71‑1 (MQ=255) cTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCg < 1:1819726/71‑1 (MQ=255) ggCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg > 1:5348665/1‑70 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc < 1:380373/53‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc < 1:3638016/53‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2118921/1‑60 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2125503/1‑60 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa < 1:4982701/67‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa < 1:3013841/67‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa > 1:2053805/1‑70 (MQ=255) tgttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGa < 1:3837518/68‑1 (MQ=255) tttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:4256130/1‑37 (MQ=255) tttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:1862993/1‑37 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGAt > 1:3448164/1‑69 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg < 1:5635217/71‑2 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg < 1:3771724/71‑2 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg > 1:4888777/1‑70 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:5386442/1‑58 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac > 1:2792368/1‑57 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg > 1:5605029/1‑67 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg > 1:3667326/1‑67 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCAGTTCATACACCTCTACAGAACCGACCACATGCGGCATg > 1:1734160/1‑67 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:4622432/68‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:1905987/68‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAcc > 1:2463294/1‑71 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc < 1:65811/70‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc > 1:1843578/1‑70 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc < 1:4802583/70‑1 (MQ=255) aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGa < 1:2130681/44‑1 (MQ=255) gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa < 1:4680426/41‑1 (MQ=255) aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:636680/1‑71 (MQ=255) aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:2739886/1‑71 (MQ=255) | GCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTG > minE/1260246‑1260385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |