| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,352 | A→C | 7.7% | V191G (GTA→GGA) ‡ | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,352 | 0 | A | C | 7.7% | 195.7 / 9.8 | 78 | V191G (GTA→GGA) ‡ | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (36/36); new base C (3/3); total (39/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
TCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAA > minE/1260282‑1260420 | tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:4327800/1‑71 (MQ=255)tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:4819010/1‑71 (MQ=255) ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg < 1:5527836/71‑1 (MQ=255) ccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg < 1:4607476/71‑1 (MQ=255) gCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc > 1:4129664/1‑70 (MQ=255) cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa < 1:3424540/61‑1 (MQ=255) cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca < 1:657866/71‑1 (MQ=255) acaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac < 1:1199900/71‑1 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2621935/1‑71 (MQ=255) aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt > 1:745218/1‑71 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca < 1:5497130/69‑1 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg < 1:1657138/71‑1 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg > 1:1607392/1‑71 (MQ=255) cTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCg < 1:1819726/71‑1 (MQ=255) ggCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg > 1:5348665/1‑70 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc < 1:380373/53‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAAcc < 1:3638016/53‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2125503/1‑60 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2118921/1‑60 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa < 1:3013841/67‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCa < 1:4982701/67‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa > 1:2053805/1‑70 (MQ=255) tgttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGa < 1:3837518/68‑1 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGAt > 1:3448164/1‑69 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg > 1:4888777/1‑70 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg < 1:3771724/71‑2 (MQ=255) ttCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGATcg < 1:5635217/71‑2 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:5386442/1‑58 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcac > 1:2792368/1‑57 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg > 1:5605029/1‑67 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg > 1:3667326/1‑67 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCAGTTCATACACCTCTACAGAACCGACCACATGCGGCATg > 1:1734160/1‑67 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:4622432/68‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:1905987/68‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAcc > 1:2463294/1‑71 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc < 1:65811/70‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc > 1:1843578/1‑70 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc < 1:4802583/70‑1 (MQ=255) ccAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc < 1:3754421/70‑1 (MQ=255) aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGa < 1:2130681/44‑1 (MQ=255) gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACa < 1:4680426/41‑1 (MQ=255) aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:636680/1‑71 (MQ=255) aaTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:2739886/1‑71 (MQ=255) ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCAc > 1:2469112/2‑64 (MQ=255) ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCAc > 1:4428977/2‑64 (MQ=255) ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgcg > 1:2004066/2‑67 (MQ=255) cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt < 1:2812304/64‑1 (MQ=255) cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt < 1:1127941/64‑1 (MQ=255) cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGccc < 1:3558249/71‑1 (MQ=255) gCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCg > 1:2095323/1‑71 (MQ=255) gCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCg > 1:3156104/1‑71 (MQ=255) cAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCAGCGCAGCACCgg > 1:1902837/1‑70 (MQ=255) aggaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGtt > 1:2762715/1‑71 (MQ=255) gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCAc > 1:2985231/1‑53 (MQ=255) tggTCTGCTGTTGATAAACTTCACTACCACCCACGACATACGGCATGAt > 1:4584112/2‑49 (MQ=255) ggTTTGCTGTTCATACACTTCCATCGAACCGACTAAGCGCGGCAGGATCACCTGCGCAGCACAGGTAGag > 1:866146/1‑69 (MQ=255) tCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa < 1:3517141/71‑1 (MQ=255) ctgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataat > 1:2371063/1‑71 (MQ=255) ctgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataat > 1:5492888/1‑71 (MQ=255) tgctgTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTGCGCAGCACCGGTAGagtaat < 1:5009371/70‑1 (MQ=255) tgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:2666496/45‑1 (MQ=255) tgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATc > 1:3645477/1‑71 (MQ=255) tgTTCATACACCTCTACAGAACCGACCACATGCgg < 1:702544/35‑1 (MQ=255) tgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgcc > 1:181128/1‑71 (MQ=255) ttCATACACCTCTACAGCACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaat < 1:4559352/62‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaat < 1:3231834/62‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgccgc < 1:1894208/71‑1 (MQ=255) cATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa > 1:4765835/1‑62 (MQ=255) cATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa > 1:440420/1‑62 (MQ=255) tACACCTCTACAGAACCGACCACATGCGGCATGATCa < 1:1579972/37‑1 (MQ=255) tACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgctgc < 1:4105586/47‑1 (MQ=255) cacCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGccc > 1:1769775/1‑47 (MQ=255) ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg < 1:5546190/71‑1 (MQ=255) ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg < 1:2810837/71‑1 (MQ=255) ctctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg > 1:2166735/1‑70 (MQ=255) tctACAGAACCGACCACATGCGGCATGATCACCtg < 1:5660438/35‑1 (MQ=255) ctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg < 1:3560212/68‑1 (MQ=255) ctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg < 1:949837/68‑1 (MQ=255) ctACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGaa > 1:4810591/1‑71 (MQ=255) tACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:167511/1‑35 (MQ=255) | TCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAA > minE/1260282‑1260420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |