| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,279,256 | C→G | 15.8% | A345A (GCC→GCG) ‡ | yeeJ → | adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,279,256 | 0 | C | G | 15.8% | 196.9 / 25.8 | 82 | A345A (GCC→GCG) ‡ | yeeJ | adhesin |
| Reads supporting (aligned to +/- strand): ref base C (41/28); new base G (9/4); total (50/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.06e-01 | |||||||||||
GCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCC > minE/1279186‑1279322 | gCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGcc > 1:4672137/1‑71 (MQ=255) tGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCt > 1:5298725/1‑71 (MQ=255) tACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGtt < 1:5211454/70‑1 (MQ=255) tACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:789441/1‑71 (MQ=255) aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:3480601/1‑70 (MQ=255) aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:276759/1‑70 (MQ=255) ccGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGtttg > 1:930567/1‑67 (MQ=255) cGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGtttgg > 1:582751/1‑66 (MQ=255) cGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGAt > 1:399332/1‑70 (MQ=255) gCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaa < 1:4646674/71‑1 (MQ=255) ccTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATaaa < 1:3471370/71‑1 (MQ=255) ggCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGAc > 1:622087/1‑71 (MQ=255) gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaacat < 1:2447591/70‑4 (MQ=255) gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa < 1:5044865/69‑1 (MQ=255) ccGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa < 1:212857/71‑1 (MQ=255) gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc > 1:5469915/1‑71 (MQ=255) gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc < 1:1670750/71‑1 (MQ=255) aCCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg > 1:3774442/1‑71 (MQ=255) ccTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg > 1:1661023/1‑70 (MQ=255) cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGccc > 1:2790648/1‑49 (MQ=255) cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa < 1:5462014/58‑1 (MQ=255) cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa < 1:4725793/58‑1 (MQ=255) gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg < 1:4415364/70‑1 (MQ=255) gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg < 1:5163512/70‑1 (MQ=255) cGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa < 1:4158708/71‑1 (MQ=255) gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGt < 1:74015/45‑1 (MQ=255) gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGt < 1:3053862/45‑1 (MQ=255) gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAAt > 1:4577140/1‑71 (MQ=255) tAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTg < 1:4469042/43‑1 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:4641011/1‑45 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:3612501/1‑64 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc > 1:3678873/1‑71 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc > 1:4000099/1‑71 (MQ=255) aaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGtt > 1:1547429/1‑43 (MQ=255) ggTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATg > 1:5237960/1‑71 (MQ=255) gTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc < 1:713950/71‑1 (MQ=255) tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa < 1:5048629/55‑1 (MQ=255) tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa < 1:4801790/55‑1 (MQ=255) cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc > 1:2481538/1‑69 (MQ=255) tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa < 1:4473728/47‑1 (MQ=255) tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa < 1:1009565/47‑1 (MQ=255) tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa < 1:1886378/58‑1 (MQ=255) tGAACAGTATTATGGCTATGAAGTCGGGCTGTTTGGTaaa > 1:947842/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGc > 1:132388/1‑50 (MQ=255) tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATaa > 1:1902824/1‑71 (MQ=255) tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa < 1:4411527/70‑1 (MQ=255) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:247319/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:5562060/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:4522852/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:644916/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa > 1:547210/1‑42 (MQ=19) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa > 1:3504138/1‑42 (MQ=19) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACgct > 1:1865255/1‑43 (MQ=255) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:4982896/1‑52 (MQ=255) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:5495788/1‑52 (MQ=255) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:1861234/1‑52 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa > 1:1065708/1‑43 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGAt > 1:1696726/1‑44 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGAt > 1:390284/1‑44 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAAcc > 1:3649832/1‑71 (MQ=255) aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCg < 1:2886515/71‑1 (MQ=255) gTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCa < 1:3520479/57‑1 (MQ=255) attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCg > 1:3319100/1‑40 (MQ=255) attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCg > 1:1046219/1‑40 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCa > 1:1897872/1‑54 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACGGCTgg < 1:2634890/69‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTgg < 1:690727/69‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTgg < 1:3714117/69‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGAc < 1:409899/71‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGAc > 1:3419483/1‑71 (MQ=255) tatGGCCATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATaa > 1:4468066/1‑61 (MQ=255) gCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc < 1:795407/71‑1 (MQ=255) cGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc > 1:3659153/1‑70 (MQ=255) gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACta > 1:260368/1‑71 (MQ=255) aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTaa < 1:4151459/40‑1 (MQ=255) tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtata < 1:1159844/71‑1 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa > 1:5288081/1‑49 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa > 1:1669836/1‑49 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa > 1:773287/1‑49 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAc > 1:4278948/1‑71 (MQ=255) gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAcccc > 1:3522912/1‑71 (MQ=255) tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTc > 1:1573677/1‑39 (MQ=255) tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTg < 1:1941665/54‑1 (MQ=255) tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAccccc > 1:5304496/1‑71 (MQ=255) cccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACta > 1:3542256/1‑61 (MQ=255) | GCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCC > minE/1279186‑1279322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |