| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,306,816 | G→A | 6.3% | F260F (TTC→TTT) | yeeF ← | predicted amino‑acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,306,816 | 0 | G | A | 6.3% | 233.1 / 4.1 | 79 | F260F (TTC→TTT) | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (28/45); new base A (2/3); total (30/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
CTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGAAAA > minE/1306746‑1306883 | cTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCggg < 1:4552386/71‑1 (MQ=255)cTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCggg < 1:822299/71‑1 (MQ=255) tACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAg > 1:4307789/1‑71 (MQ=255) aCCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGa > 1:2237705/1‑68 (MQ=255) cccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGt < 1:3252854/70‑1 (MQ=255) cccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGt < 1:2705401/70‑1 (MQ=255) cccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGt < 1:5312918/70‑1 (MQ=255) ccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTAc < 1:1880987/71‑1 (MQ=255) gCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAg > 1:2175576/1‑71 (MQ=255) ccgaCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAg > 1:822451/4‑71 (MQ=255) gaCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCt < 1:2339057/70‑1 (MQ=255) gaCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCt < 1:5173562/70‑1 (MQ=255) aCGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTg < 1:5211381/71‑1 (MQ=255) cGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACACCt < 1:5491982/69‑1 (MQ=255) gTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTg > 1:932665/1‑69 (MQ=255) gTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTg > 1:4251213/1‑69 (MQ=255) tACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTa > 1:2815789/1‑62 (MQ=255) cAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAgg < 1:5340198/70‑1 (MQ=255) cAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGa < 1:592404/71‑1 (MQ=255) cATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCggg < 1:1238806/52‑1 (MQ=255) cATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagt < 1:2356876/71‑1 (MQ=255) aTGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagta < 1:1585551/71‑1 (MQ=255) aTGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagta < 1:3349690/71‑1 (MQ=255) tGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagtaa < 1:1787441/71‑1 (MQ=255) tCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTg > 1:2886708/1‑70 (MQ=255) tCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTg > 1:3531869/1‑70 (MQ=255) aGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCaa < 1:837780/71‑1 (MQ=255) ggCTGTGACGCATGCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCaaa < 1:3197313/71‑1 (MQ=255) tgtgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaa > 1:5084163/1‑56 (MQ=255) tgtgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaa > 1:3547518/1‑56 (MQ=255) tgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGAt < 1:1297932/69‑1 (MQ=255) tgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGAt < 1:1530049/69‑1 (MQ=255) tgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGAt < 1:1573416/69‑1 (MQ=255) tgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATg < 1:4858311/70‑1 (MQ=255) gACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGaa > 1:5426419/1‑38 (MQ=255) gCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAAc > 1:5155977/1‑39 (MQ=12) gCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaa > 1:895273/1‑50 (MQ=255) tCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCaaa < 1:5295727/59‑1 (MQ=255) tCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCa < 1:1245659/57‑1 (MQ=255) tCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCa < 1:5386380/57‑1 (MQ=255) tCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAg < 1:633966/66‑1 (MQ=255) tCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCAt < 1:4635857/71‑1 (MQ=255) cGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAg > 1:5350938/1‑36 (MQ=255) cGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCaaa > 1:4232053/1‑57 (MQ=255) cGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGa > 1:3518418/1‑62 (MQ=255) cGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATg < 1:1048858/70‑1 (MQ=255) gATCTTTAAAGCGAGAGATATCCGGGAAGTACAGc > 1:343508/1‑35 (MQ=255) tCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATgccgc > 1:2417629/1‑70 (MQ=255) cTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATgccgcc > 1:4550932/1‑70 (MQ=255) tttAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGAt > 1:3015784/1‑54 (MQ=255) tttAAAGAGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGAt > 1:5669920/1‑54 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATgccgcc < 1:1528210/67‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATgccgc > 1:3816531/1‑66 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATgccgc > 1:3553765/1‑66 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGccgccg < 1:867860/68‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGccgccg < 1:932968/68‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGccgccg < 1:4123230/68‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGccgccg < 1:3588404/68‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGa < 1:3007535/69‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGAt < 1:4230961/70‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGAt < 1:5459875/70‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGAt < 1:3846685/70‑1 (MQ=255) tAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGAt > 1:4633378/1‑70 (MQ=255) aaaGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATc > 1:2415711/1‑70 (MQ=255) aaGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGa < 1:412353/55‑1 (MQ=255) aaGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCa < 1:1473492/70‑1 (MQ=255) aaGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCa < 1:4086339/70‑1 (MQ=255) aaGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAg < 1:2568976/71‑1 (MQ=255) aaGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAg < 1:2851323/71‑1 (MQ=255) agaTATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAg > 1:3462770/1‑47 (MQ=255) atCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCa > 1:4056702/1‑47 (MQ=255) atCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCa > 1:914147/1‑47 (MQ=255) tCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGAt < 1:3336755/44‑1 (MQ=255) tCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGt < 1:5154329/65‑1 (MQ=255) tCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGt < 1:4413776/65‑1 (MQ=255) tCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGaa < 1:4170785/71‑1 (MQ=255) tCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGaa < 1:4991797/71‑1 (MQ=255) cGGTAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGAt < 1:2620159/54‑1 (MQ=255) cGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCa > 1:1930623/1‑65 (MQ=255) cGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGaaaa < 1:3332064/71‑1 (MQ=255) cGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGaaaa < 1:2906619/71‑1 (MQ=255) cGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGaaa < 1:2765055/70‑1 (MQ=255) ggAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAg > 1:5307412/1‑55 (MQ=255) | CTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAAGATGAAGATCATGCCGCCGATCAGCGCGGTCAGGAAAA > minE/1306746‑1306883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |