| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,307,253 | G→A | 5.9% | L115L (CTA→TTA) ‡ | yeeF ← | predicted amino‑acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,307,253 | 0 | G | A | 5.9% | 254.2 / 3.7 | 84 | L115L (CTA→TTA) ‡ | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (33/46); new base A (3/2); total (36/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
CCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGG > minE/1307184‑1307322 | ccaGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAg > 1:590542/1‑70 (MQ=255)ccaGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAg > 1:3653919/1‑70 (MQ=255) caGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGaa < 1:2889049/71‑1 (MQ=255) gcgcCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATg < 1:209712/71‑1 (MQ=255) gcCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGtt < 1:3336601/71‑1 (MQ=255) caccacAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGa < 1:4515339/71‑1 (MQ=255) ccacAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatc < 1:4933914/71‑1 (MQ=255) ccacAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatc < 1:4672811/71‑1 (MQ=255) ccacAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatc < 1:2437463/71‑1 (MQ=255) ccacAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGat > 1:111523/1‑70 (MQ=255) cacaAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGtt < 1:707316/66‑1 (MQ=255) cacaAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatca < 1:1716307/71‑1 (MQ=255) caAATATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatcatc > 1:3027411/1‑71 (MQ=255) caAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatcatc < 1:2850238/71‑1 (MQ=255) caAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatcatc < 1:1724083/71‑1 (MQ=255) caAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatcatc < 1:2894500/71‑1 (MQ=255) caAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatcatc > 1:2303092/1‑71 (MQ=255) caAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGa > 1:886771/1‑66 (MQ=255) caAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGa > 1:5324891/1‑66 (MQ=255) aaCATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATg < 1:1467974/60‑1 (MQ=255) aaCATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATg < 1:4721457/60‑1 (MQ=255) aCATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgc > 1:2406952/1‑71 (MQ=255) cATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGa < 1:973296/62‑1 (MQ=255) cATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcg > 1:5364665/1‑71 (MQ=255) cACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcgc > 1:4097693/1‑68 (MQ=255) cACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcgc > 1:1986030/1‑68 (MQ=255) cACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa > 1:2977472/1‑71 (MQ=255) aCGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa > 1:2693746/1‑70 (MQ=255) cGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa > 1:4482169/1‑69 (MQ=255) gatgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa < 1:2586918/68‑1 (MQ=255) atgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGaa > 1:767571/1‑48 (MQ=255) atgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAAt > 1:2036669/1‑49 (MQ=255) atgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAAt > 1:5514547/1‑49 (MQ=255) atgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa < 1:5291204/71‑1 (MQ=255) atgggatggAAGGCAACAGAGCTTCAAAATAGATTTTCGCCAGTAGAAt > 1:3868982/1‑49 (MQ=255) tgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa > 1:1643169/1‑70 (MQ=255) tggaatggaaGGCACCAGAGCTTCAAAATAGATTTTCGCCAGCAGAATGTTGATCATCGGCGCGAACa < 1:1818989/68‑1 (MQ=255) gggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatcatc < 1:2901160/57‑1 (MQ=255) ggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGat > 1:2596483/1‑52 (MQ=255) tggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGatc < 1:2964775/50‑1 (MQ=255) tggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGat < 1:913706/49‑1 (MQ=255) aGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAAt < 1:666152/39‑1 (MQ=255) ggCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa < 1:1346579/60‑1 (MQ=255) ggCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa < 1:1315512/60‑1 (MQ=255) ggCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAg > 1:4504357/1‑68 (MQ=255) gCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCaga < 1:4528372/71‑1 (MQ=255) gCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCaga < 1:3017427/71‑1 (MQ=255) cACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:1412215/71‑1 (MQ=255) ccAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:3000696/69‑1 (MQ=255) agagCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgc > 1:1375809/1‑45 (MQ=255) agagCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaaga > 1:3644884/1‑71 (MQ=255) agCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAg < 1:948535/51‑1 (MQ=255) agCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:1827460/1‑54 (MQ=255) cTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcg < 1:62154/42‑1 (MQ=255) ttCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTc < 1:4417825/54‑1 (MQ=255) ttCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTc < 1:4890419/54‑1 (MQ=255) tCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGcgcg > 1:1330570/1‑42 (MQ=255) aaaTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac < 1:4898365/71‑1 (MQ=255) gaTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGAc > 1:910253/2‑62 (MQ=255) aaTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac > 1:3863930/1‑70 (MQ=255) aTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:1880476/1‑45 (MQ=255) aTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAgcgat < 1:2430133/58‑2 (MQ=255) tAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:2871970/55‑1 (MQ=255) tAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:2235700/55‑1 (MQ=255) tAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:3037931/55‑1 (MQ=255) aGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCAt > 1:1470460/1‑70 (MQ=255) aGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCa > 1:2110271/1‑61 (MQ=255) aGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCAt > 1:1544400/1‑70 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:996585/1‑42 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:1789257/1‑42 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATa < 1:4106017/70‑1 (MQ=255) gATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATa < 1:5051139/70‑1 (MQ=255) aTTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATaaa < 1:3428018/71‑1 (MQ=255) ttttCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAg < 1:763865/71‑1 (MQ=255) tttCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccacc > 1:5242652/1‑64 (MQ=255) cGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg < 1:2248072/70‑1 (MQ=255) gTAGAATGTTGATCATCGGCGCGCACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:105633/1‑71 (MQ=255) gTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:667762/1‑71 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:3742541/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:4259053/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:3408543/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:4748720/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:1059404/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCAACCATAAAGCCGACAGTCg < 1:5383184/71‑1 (MQ=255) aGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAACGcc > 1:4503115/1‑62 (MQ=255) aGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:2587812/1‑71 (MQ=255) | CCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGG > minE/1307184‑1307322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |