| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 228,318 | A→G | 8.9% | F129L (TTC→CTC) ‡ | phoE ← | outer membrane phosphoporin protein E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 228,318 | 0 | A | G | 8.9% | 175.9 / 7.3 | 67 | F129L (TTC→CTC) ‡ | phoE | outer membrane phosphoporin protein E |
| Reads supporting (aligned to +/- strand): ref base A (34/27); new base G (2/4); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 | |||||||||||
GCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCT > minE/228248‑228388 | gCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:4514319/71‑1 (MQ=255) cTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCgg > 1:5018650/1‑70 (MQ=255) cTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGt > 1:1537934/1‑71 (MQ=255) cTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGt > 1:67333/1‑71 (MQ=255) gcgcGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTcc < 1:5148096/70‑1 (MQ=255) gcgcGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTcc < 1:2699299/70‑1 (MQ=255) gcgcGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTcc < 1:2705790/70‑1 (MQ=255) gcgcGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTcc < 1:3800690/70‑1 (MQ=255) gcgcGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCa < 1:2094244/71‑1 (MQ=255) gTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCt > 1:4802152/1‑71 (MQ=255) gTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATAGCCACCAAATTCCGGGAACATATCGGTCAAGGCt > 1:1168160/1‑71 (MQ=255) tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:1095715/54‑1 (MQ=255) tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:2978170/54‑1 (MQ=255) tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:1959262/54‑1 (MQ=255) tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTc > 1:228010/1‑70 (MQ=255) tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTc > 1:4382516/1‑70 (MQ=255) tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTc > 1:473025/1‑70 (MQ=255) cATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCGGGGAACATATCGGTCCAGGCTTcc < 1:3334581/67‑1 (MQ=255) cATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTcc < 1:3826850/67‑1 (MQ=255) aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGt > 1:3327623/1‑70 (MQ=255) aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGt > 1:4214848/1‑70 (MQ=255) aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGt > 1:2943974/1‑70 (MQ=255) aTAAAGTTGTCGGTCTGCGACGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGt > 1:5477579/1‑70 (MQ=255) tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:1215067/46‑1 (MQ=255) tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:2691582/46‑1 (MQ=255) tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGaa < 1:1212664/46‑1 (MQ=255) tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCa < 1:2178694/71‑1 (MQ=255) aaaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACa > 1:1730454/1‑47 (MQ=255) aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGc > 1:2091362/1‑59 (MQ=255) aGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATAc < 1:410217/71‑1 (MQ=255) gTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATa > 1:681500/1‑66 (MQ=255) tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGTGAACATATCGGTCCAg < 1:200812/51‑1 (MQ=255) tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACg < 1:469558/70‑1 (MQ=255) tCGGTCTGCGCCGAGGAATCGCCAACAAATTCCGGGAACATATCGGTCCAg < 1:1776079/51‑1 (MQ=255) gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACatat < 1:3615088/40‑1 (MQ=255) tCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATAc < 1:4283894/62‑1 (MQ=255) tGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAg > 1:3546922/1‑71 (MQ=255) cgcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAg < 1:3694876/69‑1 (MQ=255) cgcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAg < 1:1326057/69‑1 (MQ=255) cgcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGt > 1:3631384/1‑71 (MQ=255) ccGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTa > 1:3812058/1‑71 (MQ=255) aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGa > 1:3286098/1‑71 (MQ=255) aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGa > 1:4810085/1‑71 (MQ=255) gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGccccc < 1:4421983/60‑1 (MQ=255) aaTCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGTCCa < 1:1473738/71‑1 (MQ=255) aTCGCCACCAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATa > 1:5650054/1‑48 (MQ=14) aTCGCCACCAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATa > 1:3918596/1‑48 (MQ=14) aTCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCa > 1:5461541/1‑70 (MQ=255) tCGCCACCAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:3091891/71‑1 (MQ=2) tCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATa < 1:223126/71‑1 (MQ=255) tCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATa < 1:1489484/71‑1 (MQ=255) cGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGccc > 1:5226986/1‑54 (MQ=255) cGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGccc > 1:1562158/1‑54 (MQ=255) gCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACaa > 1:368172/1‑48 (MQ=255) gCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACaa > 1:4533659/1‑48 (MQ=255) ccaccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCa > 1:4851033/1‑42 (MQ=255) caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATc > 1:2613759/1‑70 (MQ=255) acctAATTCCGGGAACTTTTCGGTACAGGCTTCCTCGTCATAc < 1:5594220/43‑1 (MQ=19) accaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGa < 1:5584933/71‑1 (MQ=255) ccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGt < 1:1350133/55‑1 (MQ=255) ccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGaa < 1:23206/71‑1 (MQ=255) caAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACaa > 1:4896614/1‑43 (MQ=255) caAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACaa > 1:5352103/1‑43 (MQ=255) aaaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATa > 1:3223565/1‑39 (MQ=255) aaaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATa > 1:2031837/1‑39 (MQ=255) aaaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGa > 1:5473907/1‑68 (MQ=255) aaaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAg < 1:886751/71‑1 (MQ=255) aaaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAg < 1:4996157/71‑1 (MQ=255) aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAg < 1:2273794/70‑1 (MQ=255) aTTCAGGGAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGa < 1:1140555/70‑1 (MQ=255) tCCGGGAACATATCGGTCCAGGCTTCCACGTCATa > 1:4964631/1‑35 (MQ=255) tCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTAc > 1:694195/1‑52 (MQ=255) ggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCaaa < 1:3284920/71‑1 (MQ=255) ggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCaaa < 1:1674353/71‑1 (MQ=255) aCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCt > 1:1659261/1‑71 (MQ=255) | GCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCT > minE/228248‑228388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |