| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,307,270 | G→A | 6.7% | A109V (GCG→GTG) ‡ | yeeF ← | predicted amino‑acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,307,270 | 0 | G | A | 6.7% | 218.4 / 4.0 | 74 | A109V (GCG→GTG) ‡ | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (32/37); new base A (3/2); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTG > minE/1307200‑1307337 | cATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcg > 1:5364665/1‑71 (MQ=255) cACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcgc > 1:4097693/1‑68 (MQ=255) cACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcgc > 1:1986030/1‑68 (MQ=255) cACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa > 1:2977472/1‑71 (MQ=255) aCGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa > 1:2693746/1‑70 (MQ=255) cGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa > 1:4482169/1‑69 (MQ=255) gatgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGaa < 1:2586918/68‑1 (MQ=255) atgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa < 1:5291204/71‑1 (MQ=255) tgggatggAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa > 1:1643169/1‑70 (MQ=255) tggaatggaaGGCACCAGAGCTTCAAAATAGATTTTCGCCAGCAGAATGTTGATCATCGGCGCGAACa < 1:1818989/68‑1 (MQ=255) ggCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa < 1:1346579/60‑1 (MQ=255) ggCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGa < 1:1315512/60‑1 (MQ=255) ggCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAg > 1:4504357/1‑68 (MQ=255) gCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCaga < 1:3017427/71‑1 (MQ=255) gCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCaga < 1:4528372/71‑1 (MQ=255) cACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:1412215/71‑1 (MQ=255) ccAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:3000696/69‑1 (MQ=255) agagCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaaga > 1:3644884/1‑71 (MQ=255) agCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAg < 1:948535/51‑1 (MQ=255) agCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:1827460/1‑54 (MQ=255) cTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcg < 1:62154/42‑1 (MQ=255) ttCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTc < 1:4417825/54‑1 (MQ=255) ttCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTc < 1:4890419/54‑1 (MQ=255) tCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGcgcg > 1:1330570/1‑42 (MQ=255) aaaTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac < 1:4898365/71‑1 (MQ=255) gaTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGAc > 1:910253/2‑62 (MQ=255) aaTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac > 1:3863930/1‑70 (MQ=255) aTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:1880476/1‑45 (MQ=255) aTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAgcgat < 1:2430133/58‑2 (MQ=255) tAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:3037931/55‑1 (MQ=255) tAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:2235700/55‑1 (MQ=255) tAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCagag < 1:2871970/55‑1 (MQ=255) aGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCAt > 1:1470460/1‑70 (MQ=255) aGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCa > 1:2110271/1‑61 (MQ=255) aGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCAt > 1:1544400/1‑70 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:996585/1‑42 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATa > 1:1789257/1‑42 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATa < 1:4106017/70‑1 (MQ=255) gATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATa < 1:5051139/70‑1 (MQ=255) aTTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATaaa < 1:3428018/71‑1 (MQ=255) ttttCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAg < 1:763865/71‑1 (MQ=255) tttCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccacc > 1:5242652/1‑64 (MQ=255) cGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg < 1:2248072/70‑1 (MQ=255) gTAGAATGTTGATCATCGGCGCGCACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:105633/1‑71 (MQ=255) gTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:667762/1‑71 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:4748720/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:4259053/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:3408543/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:1059404/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:3742541/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCAACCATAAAGCCGACAGTCg < 1:5383184/71‑1 (MQ=255) aGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAACGcc > 1:4503115/1‑62 (MQ=255) aGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:2587812/1‑71 (MQ=255) aTGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:2251210/71‑1 (MQ=255) aTGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:2625261/71‑1 (MQ=255) gTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTaa > 1:2875201/1‑71 (MQ=255) ttGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTaa < 1:4386829/70‑1 (MQ=255) ttGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAAt < 1:3691516/71‑1 (MQ=255) ttGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAAt < 1:1330337/71‑1 (MQ=255) tGATCATCGGCGCGAACAGATAGTCGAGCAGagaa > 1:1076955/1‑35 (MQ=255) tGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGt > 1:4004448/1‑61 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAAccc > 1:4920409/1‑43 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATg < 1:392577/70‑1 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATg > 1:1177148/1‑70 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATg < 1:1677791/70‑1 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGa > 1:4126299/1‑53 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGa > 1:4278524/1‑53 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGa > 1:4463388/1‑53 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACa < 1:4916529/55‑1 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGCCCAACCCCCCATAAAGCCGACAGTCGGGCTaa > 1:4688343/1‑63 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCTACCATAAAGCCGACAGt > 1:1236954/1‑55 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:2563448/1‑58 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:949094/1‑58 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTc > 1:3012123/1‑71 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTc < 1:1068143/71‑1 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCCCCATaaa > 1:4536053/1‑44 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCt < 1:2195351/70‑1 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCt < 1:3162498/70‑1 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTg > 1:4553048/1‑71 (MQ=255) gcgcGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg < 1:1780573/47‑1 (MQ=255) | CATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTG > minE/1307200‑1307337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |