| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,307,293 | A→T | 9.5% | S101S (TCT→TCA) | yeeF ← | predicted amino‑acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,307,293 | 0 | A | T | 9.5% | 169.7 / 9.2 | 63 | S101S (TCT→TCA) | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base A (28/29); new base T (3/3); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
AGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGT > minE/1307225‑1307348 | agagCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaaga > 1:3644884/1‑71 (MQ=255) aaaTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac < 1:4898365/71‑1 (MQ=255) gaTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGAc > 1:910253/2‑62 (MQ=255) aaTAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac > 1:3863930/1‑70 (MQ=255) aGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCAt > 1:1470460/1‑70 (MQ=255) aGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCa > 1:2110271/1‑61 (MQ=255) aGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCAt > 1:1544400/1‑70 (MQ=255) gATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATa < 1:4106017/70‑1 (MQ=255) gATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATa < 1:5051139/70‑1 (MQ=255) aTTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATaaa < 1:3428018/71‑1 (MQ=255) ttttCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAg < 1:763865/71‑1 (MQ=255) tttCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccacc > 1:5242652/1‑64 (MQ=255) cGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg < 1:2248072/70‑1 (MQ=255) gTAGAATGTTGATCATCGGCGCGCACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:105633/1‑71 (MQ=255) gTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:667762/1‑71 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:3742541/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:4259053/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:3408543/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:4748720/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGagaa < 1:1059404/43‑1 (MQ=255) tAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCAACCATAAAGCCGACAGTCg < 1:5383184/71‑1 (MQ=255) aGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAACGcc > 1:4503115/1‑62 (MQ=255) aGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:2587812/1‑71 (MQ=255) aTGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:2625261/71‑1 (MQ=255) aTGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:2251210/71‑1 (MQ=255) gTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTaa > 1:2875201/1‑71 (MQ=255) ttGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTaa < 1:4386829/70‑1 (MQ=255) ttGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAAt < 1:3691516/71‑1 (MQ=255) ttGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAAt < 1:1330337/71‑1 (MQ=255) tGATCATCGGCGCGAACAGATAGTCGAGCAGagaa > 1:1076955/1‑35 (MQ=255) tGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGt > 1:4004448/1‑61 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAAccc > 1:4920409/1‑43 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATg < 1:1677791/70‑1 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATg > 1:1177148/1‑70 (MQ=255) gATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATg < 1:392577/70‑1 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGa > 1:4463388/1‑53 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGa > 1:4278524/1‑53 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGa > 1:4126299/1‑53 (MQ=255) catcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACa < 1:4916529/55‑1 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGCCCAACCCCCCATAAAGCCGACAGTCGGGCTaa > 1:4688343/1‑63 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCTACCATAAAGCCGACAGt > 1:1236954/1‑55 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:949094/1‑58 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg > 1:2563448/1‑58 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTc < 1:1068143/71‑1 (MQ=255) tcGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTc > 1:3012123/1‑71 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCCCCATaaa > 1:4536053/1‑44 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCt < 1:2195351/70‑1 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCt < 1:3162498/70‑1 (MQ=255) ggCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTg > 1:4553048/1‑71 (MQ=255) gcgcGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg < 1:1780573/47‑1 (MQ=255) aCAGATAGTCGAGCAGATAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGt > 1:3487932/1‑69 (MQ=255) aCAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGt > 1:4094206/1‑69 (MQ=255) aCAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGt > 1:5150024/1‑69 (MQ=255) aCAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGt > 1:3806044/1‑69 (MQ=255) aCAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTaa < 1:1115919/71‑1 (MQ=255) cAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc > 1:1711269/1‑46 (MQ=255) gATAGTCGAGCAGAGATGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtg < 1:3934290/71‑1 (MQ=255) gATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTa < 1:1989448/50‑1 (MQ=255) gATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGt < 1:560737/66‑1 (MQ=255) gATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtg < 1:913097/71‑1 (MQ=255) gATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtg > 1:748109/1‑71 (MQ=255) gATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGAGTAAgt < 1:5576616/70‑1 (MQ=255) aTAGTCGAGCAGAGAAGACCAACGCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtgt < 1:4062448/71‑1 (MQ=255) aTAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc < 1:5528301/43‑1 (MQ=255) aTAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc < 1:4057712/43‑1 (MQ=255) aTAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTc < 1:2875575/43‑1 (MQ=255) aTAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtg > 1:382116/1‑70 (MQ=255) gTCGAGCAGAGATGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtg < 1:4155727/67‑1 (MQ=255) gagaAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:1795077/37‑1 (MQ=255) gagaAGACCAACCCACCATAAAGCCGACAGTCGGGCt < 1:3771317/37‑1 (MQ=255) agaagaCCAACCCACCATAAAGCCGACAGTCGGGCt < 1:4256360/36‑1 (MQ=255) agaagaCCAACCCACCATAAAGCCGACAGTCGGGCt < 1:2773797/36‑1 (MQ=255) agaagaCCAACCCACCATAAAGCCGACAGTCGGGCTAATGGAttt < 1:3824735/45‑1 (MQ=255) | AGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGT > minE/1307225‑1307348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |