| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,327,163 | T→C | 30.9% | intergenic (‑153/+123) | wcaK ← / ← wzxC | predicted pyruvyl transferase/colanic acid exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,327,163 | 0 | T | C | 30.9% | 71.8 / 28.4 | 42 | intergenic (‑153/+123) | wcaK/wzxC | predicted pyruvyl transferase/colanic acid exporter |
| Reads supporting (aligned to +/- strand): ref base T (11/18); new base C (3/10); total (14/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAG > minE/1327093‑1327226 | tCTTATCAGCCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:1924439/71‑1 (MQ=255) ttATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt > 1:2375478/1‑69 (MQ=255) tCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTg > 1:4129314/1‑71 (MQ=255) ccTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:1270555/71‑1 (MQ=255) cTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGAt > 1:2301945/1‑71 (MQ=255) tACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCa > 1:2859225/1‑61 (MQ=255) aaGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGa < 1:23414/71‑1 (MQ=255) caaaaCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGt > 1:1589576/4‑70 (MQ=255) caGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:4478341/44‑1 (MQ=255) aGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:3749507/43‑1 (MQ=255) aGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTc < 1:2551577/44‑1 (MQ=255) gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTc < 1:1346503/43‑1 (MQ=255) gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCtt < 1:3948198/71‑1 (MQ=255) accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCtt < 1:347533/68‑1 (MQ=11) accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCt > 1:895719/4‑70 (MQ=11) cccGTAGGACGTATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt < 1:2099847/70‑1 (MQ=11) cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTa < 1:2000872/69‑1 (MQ=11) cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt < 1:2093025/70‑1 (MQ=11) aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt < 1:4782157/71‑1 (MQ=255) aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt < 1:3576398/71‑1 (MQ=255) ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc < 1:1226928/71‑1 (MQ=11) ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc < 1:1220100/71‑1 (MQ=11) cGTAGTACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc < 1:1832529/70‑1 (MQ=255) cGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCa > 1:3286212/1‑71 (MQ=11) cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa < 1:5316478/71‑1 (MQ=255) cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa < 1:2638605/71‑1 (MQ=255) gTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGc > 1:1305785/1‑43 (MQ=38) ccaGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg > 1:5340866/3‑71 (MQ=9) tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAgg > 1:4931444/1‑71 (MQ=255) tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:3563313/70‑1 (MQ=255) caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt < 1:4084969/54‑1 (MQ=2) caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:950276/69‑1 (MQ=14) caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:1941816/69‑1 (MQ=14) ggACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGc < 1:3405046/70‑1 (MQ=255) aCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc < 1:5170376/56‑1 (MQ=9) aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc > 1:3858079/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc > 1:3192198/1‑71 (MQ=255) ttCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa < 1:5477899/53‑1 (MQ=255) cACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg > 1:3742831/1‑52 (MQ=255) cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTccc < 1:2828115/60‑1 (MQ=255) cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAAc < 1:3956110/71‑1 (MQ=255) cATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCg < 1:4400215/71‑1 (MQ=255) tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc < 1:4169605/41‑1 (MQ=255) tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc < 1:478267/41‑1 (MQ=255) cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGaa > 1:1200941/1‑64 (MQ=255) cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg > 1:2889671/1‑70 (MQ=255) ttCAGTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg < 1:1815605/65‑1 (MQ=255) ttCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGca < 1:1959955/54‑1 (MQ=255) | TCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAG > minE/1327093‑1327226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |